Restriction map for
pF1KB9644
(Linear) (Six-base) MAPSORT of: KIBB9644.seq Check: 4653 from: 1 to: 537
KIBB9644.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 287 537
Size: 194 93 250
Fragments arranged by size:
250 194 93
AleI CACnn'nnGTG
Cuts at: 0 520 537
Size: 520 17
AlwNI CAG_nnn'CTG
Cuts at: 0 91 408 537
Size: 91 317 129
Fragments arranged by size:
317 129 91
ApaI G_GGCC'C
Cuts at: 0 233 498 537
Size: 233 265 39
Fragments arranged by size:
265 233 39
AvaI C'yCGr_G
Cuts at: 0 191 226 310 365 481 491 537
Size: 191 35 84 55 116 10 46
Fragments arranged by size:
191 116 84 55 46 35 10
BamHI G'GATC_C
Cuts at: 0 503 537
Size: 503 34
BanI G'GyrC_C
Cuts at: 0 48 537
Size: 48 489
BanII G_rGCy'C
Cuts at: 0 85 112 233 498 537
Size: 85 27 121 265 39
Fragments arranged by size:
265 121 85 39 27
BglI GCCn_nnn'nGGC
Cuts at: 0 208 228 293 537
Size: 208 20 65 244
Fragments arranged by size:
244 208 65 20
BlpI GC'TnA_GC
Cuts at: 0 84 537
Size: 84 453
Bme1580I G_kGCm'C
Cuts at: 0 233 498 537
Size: 233 265 39
Fragments arranged by size:
265 233 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 41 537
Size: 41 496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 537
Size: 235 302
Bpu10I CC'TnA_GC
Cuts at: 0 92 143 537
Size: 92 51 394
Fragments arranged by size:
394 92 51
BsaWI w'CCGG_w
Cuts at: 0 54 537
Size: 54 483
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 537
Size: 102 435
BsiHKAI G_wGCw'C
Cuts at: 0 85 537
Size: 85 452
BsmBI CGTCTCn'nnnn_
Cuts at: 0 307 537
Size: 307 230
Bsp1286I G_dGCh'C
Cuts at: 0 85 112 233 498 537
Size: 85 27 121 265 39
Fragments arranged by size:
265 121 85 39 27
BsrFI r'CCGG_y
Cuts at: 0 73 292 537
Size: 73 219 245
Fragments arranged by size:
245 219 73
BssHII G'CGCG_C
Cuts at: 0 271 537
Size: 271 266
BstYI r'GATC_y
Cuts at: 0 503 537
Size: 503 34
Bsu36I CC'TnA_GG
Cuts at: 0 254 537
Size: 254 283
BtgI C'CryG_G
Cuts at: 0 41 164 206 537
Size: 41 123 42 331
Fragments arranged by size:
331 123 42 41
EaeI y'GGCC_r
Cuts at: 0 26 537
Size: 26 511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 310 496 516 537
Size: 310 186 20 21
Fragments arranged by size:
310 186 21 20
EcoICRI GAG'CTC
Cuts at: 0 83 537
Size: 83 454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 235 287 537
Size: 194 41 52 250
Fragments arranged by size:
250 194 52 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 537
Size: 481 56
EcoO109I rG'GnC_Cy
Cuts at: 0 286 361 537
Size: 286 75 176
Fragments arranged by size:
286 176 75
FspI TGC'GCA
Cuts at: 0 526 537
Size: 526 11
FspAI rTGC'GCAy
Cuts at: 0 526 537
Size: 526 11
HaeII r_GCGC'y
Cuts at: 0 253 537
Size: 253 284
MslI CAynn'nnrTG
Cuts at: 0 520 537
Size: 520 17
MspA1I CmG'CkG
Cuts at: 0 43 166 208 384 465 537
Size: 43 123 42 176 81 72
Fragments arranged by size:
176 123 81 72 43 42
NaeI GCC'GGC
Cuts at: 0 294 537
Size: 294 243
NgoMIV G'CCGG_C
Cuts at: 0 292 537
Size: 292 245
PpuMI rG'GwC_Cy
Cuts at: 0 361 537
Size: 361 176
PspOMI G'GGCC_C
Cuts at: 0 229 494 537
Size: 229 265 43
Fragments arranged by size:
265 229 43
PvuII CAG'CTG
Cuts at: 0 384 465 537
Size: 384 81 72
Fragments arranged by size:
384 81 72
SacI G_AGCT'C
Cuts at: 0 85 537
Size: 85 452
SacII CC_GC'GG
Cuts at: 0 44 167 209 537
Size: 44 123 42 328
Fragments arranged by size:
328 123 44 42
SanDI GG'GwC_CC
Cuts at: 0 361 537
Size: 361 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 537
Size: 208 329
SmaI CCC'GGG
Cuts at: 0 367 537
Size: 367 170
StuI AGG'CCT
Cuts at: 0 127 537
Size: 127 410
StyI C'CwwG_G
Cuts at: 0 428 537
Size: 428 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 537
Size: 295 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 21 53 537
Size: 21 32 484
Fragments arranged by size:
484 32 21
Tth111I GACn'n_nGTC
Cuts at: 0 305 537
Size: 305 232
XbaI T'CTAG_A
Cuts at: 0 298 537
Size: 298 239
XmaI C'CCGG_G
Cuts at: 0 365 537
Size: 365 172
Enzymes that do cut:
AcuI AleI AlwNI ApaI AvaI BamHI BanI BanII
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaWI BseRI
BsiHKAI BsmBI Bsp1286I BsrFI BssHII BstYI Bsu36I BtgI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII MslI MspA1I NaeI NgoMIV PpuMI PspOMI PvuII
SacI SacII SanDI SfiI SmaI StuI StyI TaqII
TstI Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV
FalI FseI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI XcmI XhoI XmnI ZraI