Restriction map for
pF1KB9640
(Linear) (Six-base) MAPSORT of: KIBB9640.seq Check: 3121 from: 1 to: 1083
KIBB9640.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1083
Size: 763 320
AflIII A'CryG_T
Cuts at: 0 297 395 674 1083
Size: 297 98 279 409
Fragments arranged by size:
409 297 279 98
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 287 1083
Size: 287 796
AlwNI CAG_nnn'CTG
Cuts at: 0 483 1083
Size: 483 600
ApaI G_GGCC'C
Cuts at: 0 196 1083
Size: 196 887
AvaI C'yCGr_G
Cuts at: 0 157 247 1083
Size: 157 90 836
Fragments arranged by size:
836 157 90
BanI G'GyrC_C
Cuts at: 0 354 1083
Size: 354 729
BanII G_rGCy'C
Cuts at: 0 196 506 529 796 1083
Size: 196 310 23 267 287
Fragments arranged by size:
310 287 267 196 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 993 1083
Size: 263 730 90
Fragments arranged by size:
730 263 90
BglI GCCn_nnn'nGGC
Cuts at: 0 343 352 1083
Size: 343 9 731
Fragments arranged by size:
731 343 9
BglII A'GATC_T
Cuts at: 0 920 1083
Size: 920 163
BlpI GC'TnA_GC
Cuts at: 0 718 1083
Size: 718 365
Bme1580I G_kGCm'C
Cuts at: 0 196 661 1083
Size: 196 465 422
Fragments arranged by size:
465 422 196
BmgBI CAC'GTC
Cuts at: 0 240 374 642 1083
Size: 240 134 268 441
Fragments arranged by size:
441 268 240 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 277 532 1083
Size: 177 100 255 551
Fragments arranged by size:
551 255 177 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 1083
Size: 942 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 787 898 928 1083
Size: 443 344 111 30 155
Fragments arranged by size:
443 344 155 111 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1083
Size: 877 206
BsaAI yAC'GTr
Cuts at: 0 300 1083
Size: 300 783
BsaHI Gr'CG_yC
Cuts at: 0 562 1083
Size: 562 521
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 441 471 1083
Size: 441 30 612
Fragments arranged by size:
612 441 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 784 915 1083
Size: 571 213 131 168
Fragments arranged by size:
571 213 168 131
BseYI C'CCAG_C
Cuts at: 0 139 654 1083
Size: 139 515 429
Fragments arranged by size:
515 429 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 469 803 1083
Size: 458 11 334 280
Fragments arranged by size:
458 334 280 11
BsiHKAI G_wGCw'C
Cuts at: 0 497 1083
Size: 497 586
BsmI GAATG_Cn'
Cuts at: 0 29 1083
Size: 29 1054
BsmBI CGTCTCn'nnnn_
Cuts at: 0 553 874 1083
Size: 553 321 209
Fragments arranged by size:
553 321 209
Bsp1286I G_dGCh'C
Cuts at: 0 196 497 506 529 661 796 1083
Size: 196 301 9 23 132 135 287
Fragments arranged by size:
301 287 196 135 132 23 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 689 1083
Size: 269 420 394
Fragments arranged by size:
420 394 269
BsrBI CCG'CTC
Cuts at: 0 290 425 1083
Size: 290 135 658
Fragments arranged by size:
658 290 135
BsrDI GCAATG_nn'
Cuts at: 0 150 534 1083
Size: 150 384 549
Fragments arranged by size:
549 384 150
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 1083
Size: 492 591
BstEII G'GTnAC_C
Cuts at: 0 171 1083
Size: 171 912
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 1083
Size: 225 858
BstYI r'GATC_y
Cuts at: 0 920 1083
Size: 920 163
BtgI C'CryG_G
Cuts at: 0 196 1083
Size: 196 887
DraIII CAC_nnn'GTG
Cuts at: 0 171 492 1053 1083
Size: 171 321 561 30
Fragments arranged by size:
561 321 171 30
EaeI y'GGCC_r
Cuts at: 0 2 212 381 1083
Size: 2 210 169 702
Fragments arranged by size:
702 210 169 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 790 1001 1083
Size: 790 211 82
Fragments arranged by size:
790 211 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 763 787 898 928 1083
Size: 443 320 24 111 30 155
Fragments arranged by size:
443 320 155 111 30 24
EcoO109I rG'GnC_Cy
Cuts at: 0 192 1083
Size: 192 891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 642 1083
Size: 642 441
HaeII r_GCGC'y
Cuts at: 0 655 1083
Size: 655 428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 1083
Size: 267 32 784
Fragments arranged by size:
784 267 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 575 929 1083
Size: 560 15 354 154
Fragments arranged by size:
560 354 154 15
MscI TGG'CCA
Cuts at: 0 4 214 1083
Size: 4 210 869
Fragments arranged by size:
869 210 4
MslI CAynn'nnrTG
Cuts at: 0 856 1083
Size: 856 227
MspA1I CmG'CkG
Cuts at: 0 10 463 717 1083
Size: 10 453 254 366
Fragments arranged by size:
453 366 254 10
NcoI C'CATG_G
Cuts at: 0 196 1083
Size: 196 887
NspI r_CATG'y
Cuts at: 0 75 432 1083
Size: 75 357 651
Fragments arranged by size:
651 357 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 338 1083
Size: 338 745
PspOMI G'GGCC_C
Cuts at: 0 192 1083
Size: 192 891
PstI C_TGCA'G
Cuts at: 0 700 1083
Size: 700 383
PvuII CAG'CTG
Cuts at: 0 10 463 717 1083
Size: 10 453 254 366
Fragments arranged by size:
453 366 254 10
SfcI C'TryA_G
Cuts at: 0 24 696 1083
Size: 24 672 387
Fragments arranged by size:
672 387 24
SmaI CCC'GGG
Cuts at: 0 249 1083
Size: 249 834
SmlI C'TyrA_G
Cuts at: 0 892 1083
Size: 892 191
SphI G_CATG'C
Cuts at: 0 75 432 1083
Size: 75 357 651
Fragments arranged by size:
651 357 75
SrfI GCCC'GGGC
Cuts at: 0 249 1083
Size: 249 834
StuI AGG'CCT
Cuts at: 0 709 1083
Size: 709 374
StyI C'CwwG_G
Cuts at: 0 196 1083
Size: 196 887
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 259 1083
Size: 159 100 824
Fragments arranged by size:
824 159 100
TatI w'GTAC_w
Cuts at: 0 398 1083
Size: 398 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 441 473 1083
Size: 441 32 610
Fragments arranged by size:
610 441 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 1083
Size: 222 861
XmaI C'CCGG_G
Cuts at: 0 247 1083
Size: 247 836
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbsI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstEII BstXI BstYI BtgI DraIII EaeI EciI Eco57MI
EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I
NcoI NspI PflMI PspOMI PstI PvuII SfcI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BmtI Bpu10I BsaI BsaBI BsaWI BsiEI BsiWI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI