Restriction map for
pF1KB9636
(Linear) (Six-base) MAPSORT of: KIBB9636.seq Check: 7890 from: 1 to: 816
KIBB9636.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 816
Size: 277 539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 816
Size: 301 515
ApaLI G'TGCA_C
Cuts at: 0 532 541 816
Size: 532 9 275
Fragments arranged by size:
532 275 9
ApoI r'AATT_y
Cuts at: 0 3 816
Size: 3 813
AvaI C'yCGr_G
Cuts at: 0 154 816
Size: 154 662
BanI G'GyrC_C
Cuts at: 0 178 619 651 816
Size: 178 441 32 165
Fragments arranged by size:
441 178 165 32
BanII G_rGCy'C
Cuts at: 0 19 816
Size: 19 797
BbeI G_GCGC'C
Cuts at: 0 182 655 816
Size: 182 473 161
Fragments arranged by size:
473 182 161
BglI GCCn_nnn'nGGC
Cuts at: 0 778 816
Size: 778 38
BglII A'GATC_T
Cuts at: 0 449 816
Size: 449 367
Bme1580I G_kGCm'C
Cuts at: 0 536 545 816
Size: 536 9 271
Fragments arranged by size:
536 271 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 708 816
Size: 708 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 442 816
Size: 9 433 374
Fragments arranged by size:
433 374 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 796 816
Size: 631 165 20
Fragments arranged by size:
631 165 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 123 816
Size: 123 693
BsaHI Gr'CG_yC
Cuts at: 0 179 652 816
Size: 179 473 164
Fragments arranged by size:
473 179 164
BsaWI w'CCGG_w
Cuts at: 0 42 816
Size: 42 774
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 816
Size: 439 377
BseYI C'CCAG_C
Cuts at: 0 678 816
Size: 678 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 816
Size: 705 111
BsiEI CG_ry'CG
Cuts at: 0 503 794 816
Size: 503 291 22
Fragments arranged by size:
503 291 22
BsiHKAI G_wGCw'C
Cuts at: 0 19 536 545 816
Size: 19 517 9 271
Fragments arranged by size:
517 271 19 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 816
Size: 112 704
Bsp1286I G_dGCh'C
Cuts at: 0 19 536 545 816
Size: 19 517 9 271
Fragments arranged by size:
517 271 19 9
BspEI T'CCGG_A
Cuts at: 0 42 816
Size: 42 774
BsrBI CCG'CTC
Cuts at: 0 760 816
Size: 760 56
BsrFI r'CCGG_y
Cuts at: 0 502 816
Size: 502 314
BssHII G'CGCG_C
Cuts at: 0 560 816
Size: 560 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 540 728 816
Size: 150 390 188 88
Fragments arranged by size:
390 188 150 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 816
Size: 420 396
BstYI r'GATC_y
Cuts at: 0 449 816
Size: 449 367
Bsu36I CC'TnA_GG
Cuts at: 0 64 816
Size: 64 752
BtgI C'CryG_G
Cuts at: 0 188 305 488 816
Size: 188 117 183 328
Fragments arranged by size:
328 188 183 117
BtsI GCAGTG_nn'
Cuts at: 0 477 816
Size: 477 339
DraIII CAC_nnn'GTG
Cuts at: 0 540 816
Size: 540 276
EaeI y'GGCC_r
Cuts at: 0 500 504 521 791 816
Size: 500 4 17 270 25
Fragments arranged by size:
500 270 25 17 4
EagI C'GGCC_G
Cuts at: 0 500 791 816
Size: 500 291 25
Fragments arranged by size:
500 291 25
EarI CTCTTCn'nnn_
Cuts at: 0 427 816
Size: 427 389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 585 816
Size: 585 231
EcoICRI GAG'CTC
Cuts at: 0 17 816
Size: 17 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 277 442 816
Size: 9 268 165 374
Fragments arranged by size:
374 268 165 9
EcoO109I rG'GnC_Cy
Cuts at: 0 237 816
Size: 237 579
EcoRI G'AATT_C
Cuts at: 0 3 816
Size: 3 813
FseI GG_CCGG'CC
Cuts at: 0 506 816
Size: 506 310
FspI TGC'GCA
Cuts at: 0 355 816
Size: 355 461
HaeII r_GCGC'y
Cuts at: 0 182 655 816
Size: 182 473 161
Fragments arranged by size:
473 182 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 816
Size: 1 32 783
Fragments arranged by size:
783 32 1
KasI G'GCGC_C
Cuts at: 0 178 651 816
Size: 178 473 165
Fragments arranged by size:
473 178 165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 816
Size: 209 607
MslI CAynn'nnrTG
Cuts at: 0 403 816
Size: 403 413
MspA1I CmG'CkG
Cuts at: 0 190 225 591 678 686 816
Size: 190 35 366 87 8 130
Fragments arranged by size:
366 190 130 87 35 8
NaeI GCC'GGC
Cuts at: 0 504 816
Size: 504 312
NarI GG'CG_CC
Cuts at: 0 179 652 816
Size: 179 473 164
Fragments arranged by size:
473 179 164
NcoI C'CATG_G
Cuts at: 0 305 816
Size: 305 511
NgoMIV G'CCGG_C
Cuts at: 0 502 816
Size: 502 314
PpuMI rG'GwC_Cy
Cuts at: 0 237 816
Size: 237 579
PstI C_TGCA'G
Cuts at: 0 677 816
Size: 677 139
PvuII CAG'CTG
Cuts at: 0 225 678 816
Size: 225 453 138
Fragments arranged by size:
453 225 138
SacI G_AGCT'C
Cuts at: 0 19 816
Size: 19 797
SacII CC_GC'GG
Cuts at: 0 191 816
Size: 191 625
SfcI C'TryA_G
Cuts at: 0 241 673 816
Size: 241 432 143
Fragments arranged by size:
432 241 143
SfoI GGC'GCC
Cuts at: 0 180 653 816
Size: 180 473 163
Fragments arranged by size:
473 180 163
SmaI CCC'GGG
Cuts at: 0 156 816
Size: 156 660
SmlI C'TyrA_G
Cuts at: 0 646 811 816
Size: 646 165 5
Fragments arranged by size:
646 165 5
StyI C'CwwG_G
Cuts at: 0 182 305 800 816
Size: 182 123 495 16
Fragments arranged by size:
495 182 123 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 105 816
Size: 105 711
TatI w'GTAC_w
Cuts at: 0 206 816
Size: 206 610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 774 816
Size: 700 74 42
Fragments arranged by size:
700 74 42
XmaI C'CCGG_G
Cuts at: 0 154 816
Size: 154 662
Enzymes that do cut:
AcuI AlfI ApaLI ApoI AvaI BanI BanII BbeI
BglI BglII Bme1580I BmrI BpmI BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I KasI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV PpuMI
PstI PvuII SacI SacII SfcI SfoI SmaI SmlI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaXI BsiWI BsmI BspHI BspMI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoNI EcoRV FalI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI