Restriction map for
pF1KB9632
(Linear) (Six-base) MAPSORT of: KIBB9632.seq Check: 5159 from: 1 to: 642
KIBB9632.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 507 642
Size: 507 135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 642
Size: 301 341
ApaI G_GGCC'C
Cuts at: 0 171 642
Size: 171 471
AvaI C'yCGr_G
Cuts at: 0 15 171 496 574 642
Size: 15 156 325 78 68
Fragments arranged by size:
325 156 78 68 15
BanI G'GyrC_C
Cuts at: 0 456 642
Size: 456 186
BanII G_rGCy'C
Cuts at: 0 171 613 642
Size: 171 442 29
Fragments arranged by size:
442 171 29
BbeI G_GCGC'C
Cuts at: 0 460 642
Size: 460 182
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 642
Size: 83 559
Bme1580I G_kGCm'C
Cuts at: 0 171 189 642
Size: 171 18 453
Fragments arranged by size:
453 171 18
BmgBI CAC'GTC
Cuts at: 0 98 642
Size: 98 544
BmrI ACTGGGnnnn_n'
Cuts at: 0 513 517 642
Size: 513 4 125
Fragments arranged by size:
513 125 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 583 642
Size: 466 117 59
Fragments arranged by size:
466 117 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 642
Size: 627 15
BsaHI Gr'CG_yC
Cuts at: 0 4 311 457 642
Size: 4 307 146 185
Fragments arranged by size:
307 185 146 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 642
Size: 580 62
BseYI C'CCAG_C
Cuts at: 0 109 474 582 642
Size: 109 365 108 60
Fragments arranged by size:
365 109 108 60
BsiEI CG_ry'CG
Cuts at: 0 270 642
Size: 270 372
BsmI GAATG_Cn'
Cuts at: 0 290 642
Size: 290 352
BsmBI CGTCTCn'nnnn_
Cuts at: 0 40 361 642
Size: 40 321 281
Fragments arranged by size:
321 281 40
Bsp1286I G_dGCh'C
Cuts at: 0 171 189 613 642
Size: 171 18 424 29
Fragments arranged by size:
424 171 29 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 642
Size: 97 545
BsrBI CCG'CTC
Cuts at: 0 54 642
Size: 54 588
BsrFI r'CCGG_y
Cuts at: 0 206 453 642
Size: 206 247 189
Fragments arranged by size:
247 206 189
BtsI GCAGTG_nn'
Cuts at: 0 462 642
Size: 462 180
DrdI GACnn_nn'nnGTC
Cuts at: 0 54 642
Size: 54 588
EarI CTCTTCn'nnn_
Cuts at: 0 143 600 642
Size: 143 457 42
Fragments arranged by size:
457 143 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 160 588 642
Size: 90 70 428 54
Fragments arranged by size:
428 90 70 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 583 642
Size: 466 117 59
Fragments arranged by size:
466 117 59
EcoO109I rG'GnC_Cy
Cuts at: 0 167 168 509 642
Size: 167 1 341 133
Fragments arranged by size:
341 167 133 1
FspI TGC'GCA
Cuts at: 0 138 418 642
Size: 138 280 224
Fragments arranged by size:
280 224 138
HaeII r_GCGC'y
Cuts at: 0 403 460 642
Size: 403 57 182
Fragments arranged by size:
403 182 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 642
Size: 493 32 117
Fragments arranged by size:
493 117 32
KasI G'GCGC_C
Cuts at: 0 456 642
Size: 456 186
NaeI GCC'GGC
Cuts at: 0 208 455 642
Size: 208 247 187
Fragments arranged by size:
247 208 187
NarI GG'CG_CC
Cuts at: 0 457 642
Size: 457 185
NgoMIV G'CCGG_C
Cuts at: 0 206 453 642
Size: 206 247 189
Fragments arranged by size:
247 206 189
PpuMI rG'GwC_Cy
Cuts at: 0 509 642
Size: 509 133
PspOMI G'GGCC_C
Cuts at: 0 167 642
Size: 167 475
SanDI GG'GwC_CC
Cuts at: 0 509 642
Size: 509 133
SfoI GGC'GCC
Cuts at: 0 458 642
Size: 458 184
SgrAI Cr'CCGG_yG
Cuts at: 0 453 642
Size: 453 189
SmaI CCC'GGG
Cuts at: 0 498 576 642
Size: 498 78 66
Fragments arranged by size:
498 78 66
SmlI C'TyrA_G
Cuts at: 0 606 642
Size: 606 36
Tth111I GACn'n_nGTC
Cuts at: 0 245 642
Size: 245 397
XmaI C'CCGG_G
Cuts at: 0 496 574 642
Size: 496 78 68
Fragments arranged by size:
496 78 68
Enzymes that do cut:
AhdI AlfI ApaI AvaI BanI BanII BbeI BbsI
Bme1580I BmgBI BmrI BpmI BpuEI BsaHI BseRI BseYI
BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BtsI
DrdI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I
KasI NaeI NarI NgoMIV PpuMI PspOMI SanDI SfoI
SgrAI SmaI SmlI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmnI ZraI