Restriction map for
pF1KB9630
(Linear) (Six-base) MAPSORT of: KIBB9630.seq Check: 5666 from: 1 to: 711
KIBB9630.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 549 711
Size: 549 162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 328 370 711
Size: 328 42 341
Fragments arranged by size:
341 328 42
AscI GG'CGCG_CC
Cuts at: 0 152 711
Size: 152 559
AvaI C'yCGr_G
Cuts at: 0 167 479 711
Size: 167 312 232
Fragments arranged by size:
312 232 167
BanI G'GyrC_C
Cuts at: 0 218 475 565 711
Size: 218 257 90 146
Fragments arranged by size:
257 218 146 90
BanII G_rGCy'C
Cuts at: 0 429 562 711
Size: 429 133 149
Fragments arranged by size:
429 149 133
BbeI G_GCGC'C
Cuts at: 0 222 711
Size: 222 489
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 623 711
Size: 95 528 88
Fragments arranged by size:
528 95 88
Bme1580I G_kGCm'C
Cuts at: 0 192 480 711
Size: 192 288 231
Fragments arranged by size:
288 231 192
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 711
Size: 590 121
Bpu10I CC'TnA_GC
Cuts at: 0 561 711
Size: 561 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 711
Size: 34 677
BsaI GGTCTCn'nnnn_
Cuts at: 0 10 711
Size: 10 701
BsaHI Gr'CG_yC
Cuts at: 0 172 219 711
Size: 172 47 492
Fragments arranged by size:
492 172 47
BseYI C'CCAG_C
Cuts at: 0 603 711
Size: 603 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 711
Size: 241 470
BsiEI CG_ry'CG
Cuts at: 0 228 297 711
Size: 228 69 414
Fragments arranged by size:
414 228 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 388 711
Size: 388 323
Bsp1286I G_dGCh'C
Cuts at: 0 192 429 480 562 711
Size: 192 237 51 82 149
Fragments arranged by size:
237 192 149 82 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 711
Size: 28 683
BsrBI CCG'CTC
Cuts at: 0 242 585 711
Size: 242 343 126
Fragments arranged by size:
343 242 126
BsrFI r'CCGG_y
Cuts at: 0 138 275 711
Size: 138 137 436
Fragments arranged by size:
436 138 137
BssHII G'CGCG_C
Cuts at: 0 152 711
Size: 152 559
BstAPI GCAn_nnn'nTGC
Cuts at: 0 264 711
Size: 264 447
BtgI C'CryG_G
Cuts at: 0 222 711
Size: 222 489
BtsI GCAGTG_nn'
Cuts at: 0 63 340 439 508 711
Size: 63 277 99 69 203
Fragments arranged by size:
277 203 99 69 63
EaeI y'GGCC_r
Cuts at: 0 225 431 711
Size: 225 206 280
Fragments arranged by size:
280 225 206
EagI C'GGCC_G
Cuts at: 0 225 711
Size: 225 486
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 466 711
Size: 466 245
FspI TGC'GCA
Cuts at: 0 268 346 711
Size: 268 78 365
Fragments arranged by size:
365 268 78
HaeII r_GCGC'y
Cuts at: 0 222 253 489 711
Size: 222 31 236 222
Fragments arranged by size:
236 222 222 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 711
Size: 535 32 144
Fragments arranged by size:
535 144 32
KasI G'GCGC_C
Cuts at: 0 218 711
Size: 218 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 129 711
Size: 53 76 582
Fragments arranged by size:
582 76 53
MspA1I CmG'CkG
Cuts at: 0 53 224 711
Size: 53 171 487
Fragments arranged by size:
487 171 53
NaeI GCC'GGC
Cuts at: 0 140 277 711
Size: 140 137 434
Fragments arranged by size:
434 140 137
NarI GG'CG_CC
Cuts at: 0 219 711
Size: 219 492
NgoMIV G'CCGG_C
Cuts at: 0 138 275 711
Size: 138 137 436
Fragments arranged by size:
436 138 137
NruI TCG'CGA
Cuts at: 0 299 711
Size: 299 412
NspI r_CATG'y
Cuts at: 0 315 711
Size: 315 396
PshAI GACnn'nnGTC
Cuts at: 0 235 711
Size: 235 476
PvuI CG_AT'CG
Cuts at: 0 297 711
Size: 297 414
SacII CC_GC'GG
Cuts at: 0 225 711
Size: 225 486
SfoI GGC'GCC
Cuts at: 0 220 711
Size: 220 491
SmaI CCC'GGG
Cuts at: 0 169 481 711
Size: 169 312 230
Fragments arranged by size:
312 230 169
SmlI C'TyrA_G
Cuts at: 0 13 711
Size: 13 698
SphI G_CATG'C
Cuts at: 0 315 711
Size: 315 396
XmaI C'CCGG_G
Cuts at: 0 167 479 711
Size: 167 312 232
Fragments arranged by size:
312 232 167
Enzymes that do cut:
AhdI AlfI AscI AvaI BanI BanII BbeI BbsI
Bme1580I BmrI Bpu10I BpuEI BsaI BsaHI BseYI BsgI
BsiEI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BtgI BtsI EaeI EagI EciI FspI HaeII Hin4I
KasI MmeI MspA1I NaeI NarI NgoMIV NruI NspI
PshAI PvuI SacII SfoI SmaI SmlI SphI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI