Restriction map for
pF1KB9628
(Linear) (Six-base) MAPSORT of: KIBB9628.seq Check: 4284 from: 1 to: 993
KIBB9628.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 901 993
Size: 901 92
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 440 467 993
Size: 164 276 27 526
Fragments arranged by size:
526 276 164 27
AflIII A'CryG_T
Cuts at: 0 929 993
Size: 929 64
ApaI G_GGCC'C
Cuts at: 0 532 754 993
Size: 532 222 239
Fragments arranged by size:
532 239 222
BanI G'GyrC_C
Cuts at: 0 901 993
Size: 901 92
BanII G_rGCy'C
Cuts at: 0 79 261 485 532 754 993
Size: 79 182 224 47 222 239
Fragments arranged by size:
239 224 222 182 79 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 975 993
Size: 975 18
BfrBI ATG'CAT
Cuts at: 0 300 993
Size: 300 693
BlpI GC'TnA_GC
Cuts at: 0 130 993
Size: 130 863
Bme1580I G_kGCm'C
Cuts at: 0 532 754 993
Size: 532 222 239
Fragments arranged by size:
532 239 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 535 993
Size: 535 458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 568 993
Size: 448 120 425
Fragments arranged by size:
448 425 120
Bpu10I CC'TnA_GC
Cuts at: 0 769 867 993
Size: 769 98 126
Fragments arranged by size:
769 126 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 655 993
Size: 265 390 338
Fragments arranged by size:
390 338 265
BsaI GGTCTCn'nnnn_
Cuts at: 0 812 993
Size: 812 181
BsaHI Gr'CG_yC
Cuts at: 0 314 993
Size: 314 679
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 56 457 487 993
Size: 26 30 401 30 506
Fragments arranged by size:
506 401 30 30 26
BsmI GAATG_Cn'
Cuts at: 0 879 993
Size: 879 114
Bsp1286I G_dGCh'C
Cuts at: 0 79 261 485 532 754 993
Size: 79 182 224 47 222 239
Fragments arranged by size:
239 224 222 182 79 47
BssSI C'ACGA_G
Cuts at: 0 556 993
Size: 556 437
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 993
Size: 30 963
Bsu36I CC'TnA_GG
Cuts at: 0 328 993
Size: 328 665
EaeI y'GGCC_r
Cuts at: 0 392 435 993
Size: 392 43 558
Fragments arranged by size:
558 392 43
EarI CTCTTCn'nnn_
Cuts at: 0 95 140 170 258 993
Size: 95 45 30 88 735
Fragments arranged by size:
735 95 88 45 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 440 448 467 568 993
Size: 164 276 8 19 101 425
Fragments arranged by size:
425 276 164 101 19 8
EcoO109I rG'GnC_Cy
Cuts at: 0 211 529 750 965 993
Size: 211 318 221 215 28
Fragments arranged by size:
318 221 215 211 28
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 259 993
Size: 259 734
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 195 222 227 254 366 398
Size: 26 32 137 27 5 27 112 32
Cuts at: 398 550 582 993
Size: 152 32 411
Fragments arranged by size:
411 152 137 112 32 32 32 27
27 26 5
HincII GTy'rAC
Cuts at: 0 45 993
Size: 45 948
KpnI G_GTAC'C
Cuts at: 0 905 993
Size: 905 88
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 993
Size: 546 447
MscI TGG'CCA
Cuts at: 0 394 437 993
Size: 394 43 556
Fragments arranged by size:
556 394 43
NdeI CA'TA_TG
Cuts at: 0 647 993
Size: 647 346
NsiI A_TGCA'T
Cuts at: 0 302 993
Size: 302 691
NspI r_CATG'y
Cuts at: 0 877 933 993
Size: 877 56 60
Fragments arranged by size:
877 60 56
PciI A'CATG_T
Cuts at: 0 929 993
Size: 929 64
PfoI T'CCnGG_A
Cuts at: 0 55 425 545 993
Size: 55 370 120 448
Fragments arranged by size:
448 370 120 55
PpuMI rG'GwC_Cy
Cuts at: 0 211 965 993
Size: 211 754 28
Fragments arranged by size:
754 211 28
PspOMI G'GGCC_C
Cuts at: 0 528 750 993
Size: 528 222 243
Fragments arranged by size:
528 243 222
PstI C_TGCA'G
Cuts at: 0 733 993
Size: 733 260
SanDI GG'GwC_CC
Cuts at: 0 211 965 993
Size: 211 754 28
Fragments arranged by size:
754 211 28
SapI GCTCTTCn'nnn_
Cuts at: 0 140 993
Size: 140 853
SexAI A'CCwGG_T
Cuts at: 0 111 503 993
Size: 111 392 490
Fragments arranged by size:
490 392 111
SfcI C'TryA_G
Cuts at: 0 729 828 923 993
Size: 729 99 95 70
Fragments arranged by size:
729 99 95 70
SmlI C'TyrA_G
Cuts at: 0 244 670 993
Size: 244 426 323
Fragments arranged by size:
426 323 244
StyI C'CwwG_G
Cuts at: 0 23 993
Size: 23 970
TatI w'GTAC_w
Cuts at: 0 733 993
Size: 733 260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 457 489 993
Size: 457 32 504
Fragments arranged by size:
504 457 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 958 993
Size: 501 457 35
Fragments arranged by size:
501 457 35
Enzymes that do cut:
Acc65I AcuI AflIII ApaI BanI BanII BbsI BfrBI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BsmI Bsp1286I BssSI BstXI Bsu36I EaeI EarI
Eco57MI EcoO109I FalI Hin4I HincII KpnI MmeI MscI
NdeI NsiI NspI PciI PfoI PpuMI PspOMI PstI
SanDI SapI SexAI SfcI SmlI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BglII BmgBI BmtI BplI BsaAI
BsaBI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI