Restriction map for
pF1KB9627
(Linear) (Six-base) MAPSORT of: KIBB9627.seq Check: 4948 from: 1 to: 1146
KIBB9627.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AfeI AGC'GCT
Cuts at: 0 352 1146
Size: 352 794
AhdI GACnn_n'nnGTC
Cuts at: 0 555 1146
Size: 555 591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 415 457 1146
Size: 415 42 689
Fragments arranged by size:
689 415 42
AlwNI CAG_nnn'CTG
Cuts at: 0 468 573 1146
Size: 468 105 573
Fragments arranged by size:
573 468 105
ApaI G_GGCC'C
Cuts at: 0 317 892 1111 1146
Size: 317 575 219 35
Fragments arranged by size:
575 317 219 35
AvaI C'yCGr_G
Cuts at: 0 130 155 687 1146
Size: 130 25 532 459
Fragments arranged by size:
532 459 130 25
BanI G'GyrC_C
Cuts at: 0 441 658 667 1146
Size: 441 217 9 479
Fragments arranged by size:
479 441 217 9
BanII G_rGCy'C
Cuts at: 0 26 144 317 862 892 1111 1127 1146
Size: 26 118 173 545 30 219 16 19
Fragments arranged by size:
545 219 173 118 30 26 19 16
BbeI G_GCGC'C
Cuts at: 0 662 1146
Size: 662 484
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 1020 1146
Size: 80 940 126
Fragments arranged by size:
940 126 80
BglI GCCn_nnn'nGGC
Cuts at: 0 160 208 740 773 1146
Size: 160 48 532 33 373
Fragments arranged by size:
532 373 160 48 33
BlpI GC'TnA_GC
Cuts at: 0 895 1146
Size: 895 251
Bme1580I G_kGCm'C
Cuts at: 0 47 317 446 892 1111 1146
Size: 47 270 129 446 219 35
Fragments arranged by size:
446 270 219 129 47 35
BmgBI CAC'GTC
Cuts at: 0 42 1146
Size: 42 1104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1146
Size: 916 230
BsaAI yAC'GTr
Cuts at: 0 564 1146
Size: 564 582
BsaHI Gr'CG_yC
Cuts at: 0 659 1055 1146
Size: 659 396 91
Fragments arranged by size:
659 396 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 859 889 1146
Size: 190 30 639 30 257
Fragments arranged by size:
639 257 190 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 259 262 265 274 277 933 1135
Size: 102 157 3 3 9 3 656 202
Cuts at: 1135 1146
Size: 11
Fragments arranged by size:
656 202 157 102 11 9 3 3
3
BseYI C'CCAG_C
Cuts at: 0 59 234 1146
Size: 59 175 912
Fragments arranged by size:
912 175 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1146
Size: 586 560
BsiEI CG_ry'CG
Cuts at: 0 675 761 1104 1146
Size: 675 86 343 42
Fragments arranged by size:
675 343 86 42
BsiHKAI G_wGCw'C
Cuts at: 0 730 1127 1146
Size: 730 397 19
Fragments arranged by size:
730 397 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 475 817 1146
Size: 475 342 329
Fragments arranged by size:
475 342 329
Bsp1286I G_dGCh'C
Cuts at: 0 26 47 144 317 446 730 862 892
Size: 26 21 97 173 129 284 132 30
Cuts at: 892 1111 1127 1146
Size: 219 16 19
Fragments arranged by size:
284 219 173 132 129 97 30 26
21 19 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1146
Size: 437 709
BsrBI CCG'CTC
Cuts at: 0 297 894 906 1146
Size: 297 597 12 240
Fragments arranged by size:
597 297 240 12
BsrFI r'CCGG_y
Cuts at: 0 613 670 760 1146
Size: 613 57 90 386
Fragments arranged by size:
613 386 90 57
BssHII G'CGCG_C
Cuts at: 0 340 1146
Size: 340 806
BssSI C'ACGA_G
Cuts at: 0 955 1146
Size: 955 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 792 1146
Size: 792 354
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 609 1146
Size: 606 3 537
Fragments arranged by size:
606 537 3
BstYI r'GATC_y
Cuts at: 0 524 1146
Size: 524 622
BtgI C'CryG_G
Cuts at: 0 180 681 801 1010 1017 1146
Size: 180 501 120 209 7 129
Fragments arranged by size:
501 209 180 129 120 7
EaeI y'GGCC_r
Cuts at: 0 161 209 544 611 672 758 1146
Size: 161 48 335 67 61 86 388
Fragments arranged by size:
388 335 161 86 67 61 48
EagI C'GGCC_G
Cuts at: 0 672 758 1146
Size: 672 86 388
Fragments arranged by size:
672 388 86
EarI CTCTTCn'nnn_
Cuts at: 0 182 251 1146
Size: 182 69 895
Fragments arranged by size:
895 182 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 1146
Size: 301 845
EcoICRI GAG'CTC
Cuts at: 0 1125 1146
Size: 1125 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 433 1146
Size: 433 713
EcoO109I rG'GnC_Cy
Cuts at: 0 888 1107 1108 1146
Size: 888 219 1 38
Fragments arranged by size:
888 219 38 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 360 1146
Size: 360 786
FspI TGC'GCA
Cuts at: 0 433 1146
Size: 433 713
HaeII r_GCGC'y
Cuts at: 0 354 517 662 994 1146
Size: 354 163 145 332 152
Fragments arranged by size:
354 332 163 152 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 1146
Size: 541 32 573
Fragments arranged by size:
573 541 32
HindIII A'AGCT_T
Cuts at: 0 358 1146
Size: 358 788
KasI G'GCGC_C
Cuts at: 0 658 1146
Size: 658 488
MspA1I CmG'CkG
Cuts at: 0 59 1146
Size: 59 1087
NaeI GCC'GGC
Cuts at: 0 615 672 762 1146
Size: 615 57 90 384
Fragments arranged by size:
615 384 90 57
NarI GG'CG_CC
Cuts at: 0 659 1146
Size: 659 487
NgoMIV G'CCGG_C
Cuts at: 0 613 670 760 1146
Size: 613 57 90 386
Fragments arranged by size:
613 386 90 57
NspI r_CATG'y
Cuts at: 0 402 1146
Size: 402 744
PspOMI G'GGCC_C
Cuts at: 0 313 888 1107 1146
Size: 313 575 219 39
Fragments arranged by size:
575 313 219 39
PstI C_TGCA'G
Cuts at: 0 626 1146
Size: 626 520
PvuII CAG'CTG
Cuts at: 0 59 1146
Size: 59 1087
SacI G_AGCT'C
Cuts at: 0 1127 1146
Size: 1127 19
SfcI C'TryA_G
Cuts at: 0 622 1146
Size: 622 524
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 208 1146
Size: 208 938
SfoI GGC'GCC
Cuts at: 0 660 1146
Size: 660 486
SmaI CCC'GGG
Cuts at: 0 132 157 1146
Size: 132 25 989
Fragments arranged by size:
989 132 25
SmlI C'TyrA_G
Cuts at: 0 931 1146
Size: 931 215
SphI G_CATG'C
Cuts at: 0 402 1146
Size: 402 744
SrfI GCCC'GGGC
Cuts at: 0 132 157 1146
Size: 132 25 989
Fragments arranged by size:
989 132 25
StyI C'CwwG_G
Cuts at: 0 335 1146
Size: 335 811
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 603 606 1146
Size: 342 261 3 540
Fragments arranged by size:
540 342 261 3
XmaI C'CCGG_G
Cuts at: 0 130 155 1146
Size: 130 25 991
Fragments arranged by size:
991 130 25
Enzymes that do cut:
AfeI AhdI AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI
BstYI BtgI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I FalI FspI HaeII Hin4I HindIII KasI MspA1I
NaeI NarI NgoMIV NspI PspOMI PstI PvuII SacI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StyI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI Eco57MI EcoRI EcoRV FseI FspAI HincII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI