Restriction map for
pF1KB9626
(Linear) (Six-base) MAPSORT of: KIBB9626.seq Check: 3563 from: 1 to: 405
KIBB9626.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AleI CACnn'nnGTG
Cuts at: 0 88 405
Size: 88 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 405
Size: 325 80
AlwNI CAG_nnn'CTG
Cuts at: 0 186 405
Size: 186 219
AscI GG'CGCG_CC
Cuts at: 0 216 405
Size: 216 189
BamHI G'GATC_C
Cuts at: 0 57 405
Size: 57 348
BanII G_rGCy'C
Cuts at: 0 299 405
Size: 299 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 11 45 405
Size: 11 34 360
Fragments arranged by size:
360 34 11
BlpI GC'TnA_GC
Cuts at: 0 187 405
Size: 187 218
BmgBI CAC'GTC
Cuts at: 0 393 402 405
Size: 393 9 3
Fragments arranged by size:
393 9 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 405
Size: 181 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 405
Size: 133 272
BsaBI GATnn'nnATC
Cuts at: 0 349 405
Size: 349 56
BsaWI w'CCGG_w
Cuts at: 0 12 60 405
Size: 12 48 345
Fragments arranged by size:
345 48 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 211 405
Size: 139 72 194
Fragments arranged by size:
194 139 72
BseYI C'CCAG_C
Cuts at: 0 40 405
Size: 40 365
BsiHKAI G_wGCw'C
Cuts at: 0 95 299 405
Size: 95 204 106
Fragments arranged by size:
204 106 95
Bsp1286I G_dGCh'C
Cuts at: 0 95 299 405
Size: 95 204 106
Fragments arranged by size:
204 106 95
BspEI T'CCGG_A
Cuts at: 0 12 60 405
Size: 12 48 345
Fragments arranged by size:
345 48 12
BsrBI CCG'CTC
Cuts at: 0 236 405
Size: 236 169
BsrFI r'CCGG_y
Cuts at: 0 117 212 405
Size: 117 95 193
Fragments arranged by size:
193 117 95
BssHII G'CGCG_C
Cuts at: 0 216 405
Size: 216 189
BstYI r'GATC_y
Cuts at: 0 57 350 405
Size: 57 293 55
Fragments arranged by size:
293 57 55
BtgI C'CryG_G
Cuts at: 0 221 405
Size: 221 184
EaeI y'GGCC_r
Cuts at: 0 224 362 405
Size: 224 138 43
Fragments arranged by size:
224 138 43
EcoICRI GAG'CTC
Cuts at: 0 297 405
Size: 297 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 405
Size: 133 272
HaeII r_GCGC'y
Cuts at: 0 207 405
Size: 207 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 49 81 405
Size: 26 23 32 324
Fragments arranged by size:
324 32 26 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 405
Size: 86 319
MscI TGG'CCA
Cuts at: 0 364 405
Size: 364 41
MslI CAynn'nnrTG
Cuts at: 0 88 265 405
Size: 88 177 140
Fragments arranged by size:
177 140 88
MspA1I CmG'CkG
Cuts at: 0 186 405
Size: 186 219
NaeI GCC'GGC
Cuts at: 0 214 405
Size: 214 191
NgoMIV G'CCGG_C
Cuts at: 0 212 405
Size: 212 193
PasI CC'CwG_GG
Cuts at: 0 69 405
Size: 69 336
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 405
Size: 396 9
PfoI T'CCnGG_A
Cuts at: 0 395 405
Size: 395 10
PvuII CAG'CTG
Cuts at: 0 186 405
Size: 186 219
SacI G_AGCT'C
Cuts at: 0 299 405
Size: 299 106
SgrAI Cr'CCGG_yG
Cuts at: 0 212 405
Size: 212 193
StyI C'CwwG_G
Cuts at: 0 86 405
Size: 86 319
Enzymes that do cut:
AleI AlfI AlwNI AscI BamHI BanII BcgI BlpI
BmgBI BplI BpmI BsaBI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspEI BsrBI BsrFI BssHII BstYI BtgI EaeI
EcoICRI Eco57MI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV PasI PflMI PfoI PvuII SacI SgrAI
StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI