Restriction map for
pF1KB9624
(Linear) (Six-base) MAPSORT of: KIBB9624.seq Check: 2992 from: 1 to: 1074
KIBB9624.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 437 1074
Size: 437 637
AatII G_ACGT'C
Cuts at: 0 296 1074
Size: 296 778
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 942 1074
Size: 178 764 132
Fragments arranged by size:
764 178 132
AflIII A'CryG_T
Cuts at: 0 1005 1074
Size: 1005 69
AhdI GACnn_n'nnGTC
Cuts at: 0 602 1074
Size: 602 472
AleI CACnn'nnGTG
Cuts at: 0 676 1074
Size: 676 398
ApaI G_GGCC'C
Cuts at: 0 101 1074
Size: 101 973
AseI AT'TA_AT
Cuts at: 0 890 1074
Size: 890 184
BanI G'GyrC_C
Cuts at: 0 90 806 1074
Size: 90 716 268
Fragments arranged by size:
716 268 90
BanII G_rGCy'C
Cuts at: 0 101 860 1074
Size: 101 759 214
Fragments arranged by size:
759 214 101
BbvCI CC'TCA_GC
Cuts at: 0 529 1074
Size: 529 545
BglI GCCn_nnn'nGGC
Cuts at: 0 968 1074
Size: 968 106
BglII A'GATC_T
Cuts at: 0 176 1074
Size: 176 898
Bme1580I G_kGCm'C
Cuts at: 0 86 101 1074
Size: 86 15 973
Fragments arranged by size:
973 86 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 683 1074
Size: 683 391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 460 630 1007 1074
Size: 454 6 170 377 67
Fragments arranged by size:
454 377 170 67 6
Bpu10I CC'TnA_GC
Cuts at: 0 529 1074
Size: 529 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 557 1074
Size: 557 517
BsaAI yAC'GTr
Cuts at: 0 1008 1074
Size: 1008 66
BsaBI GATnn'nnATC
Cuts at: 0 457 1074
Size: 457 617
BsaHI Gr'CG_yC
Cuts at: 0 293 1074
Size: 293 781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 228 258 283 313 505 535 1074
Size: 228 30 25 30 192 30 539
Fragments arranged by size:
539 228 192 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 360 838 1074
Size: 291 69 478 236
Fragments arranged by size:
478 291 236 69
BseYI C'CCAG_C
Cuts at: 0 122 265 270 516 1074
Size: 122 143 5 246 558
Fragments arranged by size:
558 246 143 122 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 971 1074
Size: 865 106 103
Fragments arranged by size:
865 106 103
BsmBI CGTCTCn'nnnn_
Cuts at: 0 894 1074
Size: 894 180
Bsp1286I G_dGCh'C
Cuts at: 0 86 101 860 1074
Size: 86 15 759 214
Fragments arranged by size:
759 214 86 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 333 437 1074
Size: 333 104 637
Fragments arranged by size:
637 333 104
BsrBI CCG'CTC
Cuts at: 0 192 1074
Size: 192 882
BsrFI r'CCGG_y
Cuts at: 0 113 1074
Size: 113 961
BsrGI T'GTAC_A
Cuts at: 0 585 1074
Size: 585 489
BstXI CCAn_nnnn'nTGG
Cuts at: 0 465 1074
Size: 465 609
BstYI r'GATC_y
Cuts at: 0 176 749 1074
Size: 176 573 325
Fragments arranged by size:
573 325 176
BtgI C'CryG_G
Cuts at: 0 86 282 727 1074
Size: 86 196 445 347
Fragments arranged by size:
445 347 196 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 24 1074
Size: 24 1050
BtsI GCAGTG_nn'
Cuts at: 0 86 326 1074
Size: 86 240 748
Fragments arranged by size:
748 240 86
ClaI AT'CG_AT
Cuts at: 0 923 1074
Size: 923 151
DrdI GACnn_nn'nnGTC
Cuts at: 0 658 1074
Size: 658 416
EaeI y'GGCC_r
Cuts at: 0 764 1074
Size: 764 310
EarI CTCTTCn'nnn_
Cuts at: 0 154 199 736 1074
Size: 154 45 537 338
Fragments arranged by size:
537 338 154 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 454 460 630 942 1007 1074
Size: 178 276 6 170 312 65 67
Fragments arranged by size:
312 276 178 170 67 65 6
EcoO109I rG'GnC_Cy
Cuts at: 0 97 98 359 596 618 1074
Size: 97 1 261 237 22 456
Fragments arranged by size:
456 261 237 97 22 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 283 315 565 588 597 620
Size: 176 32 75 32 250 23 9 23
Cuts at: 620 1074
Size: 454
Fragments arranged by size:
454 250 176 75 32 32 23 23
9
HincII GTy'rAC
Cuts at: 0 876 1074
Size: 876 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 956 1074
Size: 398 558 118
Fragments arranged by size:
558 398 118
MscI TGG'CCA
Cuts at: 0 766 1074
Size: 766 308
MslI CAynn'nnrTG
Cuts at: 0 412 676 1074
Size: 412 264 398
Fragments arranged by size:
412 398 264
MspA1I CmG'CkG
Cuts at: 0 88 284 614 840 1074
Size: 88 196 330 226 234
Fragments arranged by size:
330 234 226 196 88
NspI r_CATG'y
Cuts at: 0 242 1074
Size: 242 832
PflMI CCAn_nnn'nTGG
Cuts at: 0 432 1074
Size: 432 642
PmlI CAC'GTG
Cuts at: 0 1008 1074
Size: 1008 66
PpuMI rG'GwC_Cy
Cuts at: 0 596 1074
Size: 596 478
PspOMI G'GGCC_C
Cuts at: 0 97 1074
Size: 97 977
PstI C_TGCA'G
Cuts at: 0 496 1074
Size: 496 578
PvuII CAG'CTG
Cuts at: 0 840 1074
Size: 840 234
SacII CC_GC'GG
Cuts at: 0 89 285 1074
Size: 89 196 789
Fragments arranged by size:
789 196 89
SanDI GG'GwC_CC
Cuts at: 0 596 1074
Size: 596 478
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1074
Size: 199 875
SexAI A'CCwGG_T
Cuts at: 0 464 1074
Size: 464 610
SfcI C'TryA_G
Cuts at: 0 492 1074
Size: 492 582
StyI C'CwwG_G
Cuts at: 0 654 758 1074
Size: 654 104 316
Fragments arranged by size:
654 316 104
TatI w'GTAC_w
Cuts at: 0 585 1074
Size: 585 489
Tth111I GACn'n_nGTC
Cuts at: 0 466 1074
Size: 466 608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 618 1074
Size: 381 237 456
Fragments arranged by size:
456 381 237
ZraI GAC'GTC
Cuts at: 0 294 1074
Size: 294 780
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI ApaI AseI
BanI BanII BbvCI BglI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI
BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EarI
Eco57MI EcoO109I Hin4I HincII MmeI MscI MslI MspA1I
NspI PflMI PmlI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SexAI SfcI StyI TatI Tth111I XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI BpuEI BsaWI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrDI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I CspCI DraI DraIII EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI XbaI
XhoI XmaI XmnI