(Linear) (Six-base) MAPSORT of: KIBB9623.seq  Check: 2019  from: 1  to: 438



KIBB9623.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AclI AA'CG_TT

Cuts at:      0     166     438 
   Size:        166     272
 
AvaI C'yCGr_G

Cuts at:      0     193     438 
   Size:        193     245
 
AvrII C'CTAG_G

Cuts at:      0     342     438 
   Size:        342      96
 
BanI G'GyrC_C

Cuts at:      0      44     150     312     438 
   Size:         44     106     162     126

  Fragments arranged by size: 

                 162     126     106      44
 
BanII G_rGCy'C

Cuts at:      0     184     438 
   Size:        184     254
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     410     438 
   Size:        410      28
 
Bme1580I G_kGCm'C

Cuts at:      0     155     317     438 
   Size:        155     162     121

  Fragments arranged by size: 

                 162     155     121
 
Bpu10I CC'TnA_GC

Cuts at:      0      55     438 
   Size:         55     383
 
BsaHI Gr'CG_yC

Cuts at:      0     214     254     438 
   Size:        214      40     184

  Fragments arranged by size: 

                 214     184      40
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     316     438 
   Size:        274      42     122

  Fragments arranged by size: 

                 274     122      42
 
BseYI C'CCAG_C

Cuts at:      0     389     438 
   Size:        389      49
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     226     438 
   Size:         10     216     212

  Fragments arranged by size: 

                 216     212      10
 
BsiHKAI G_wGCw'C

Cuts at:      0     184     438 
   Size:        184     254
 
BsmI GAATG_Cn'

Cuts at:      0     268     438 
   Size:        268     170
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     184     317     438 
   Size:        155      29     133     121

  Fragments arranged by size: 

                 155     133     121      29
 
BssSI C'ACGA_G

Cuts at:      0     226     438 
   Size:        226     212
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264     438 
   Size:        264     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     438 
   Size:        416      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     125     438 
   Size:        125     313
 
EcoICRI GAG'CTC

Cuts at:      0     182     438 
   Size:        182     256
 
MslI CAynn'nnrTG

Cuts at:      0     226     262     325     438 
   Size:        226      36      63     113

  Fragments arranged by size: 

                 226     113      63      36
 
MspA1I CmG'CkG

Cuts at:      0      87     438 
   Size:         87     351
 
NspI r_CATG'y

Cuts at:      0     225     438 
   Size:        225     213
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      78     438 
   Size:         78     360
 
PvuII CAG'CTG

Cuts at:      0      87     438 
   Size:         87     351
 
SacI G_AGCT'C

Cuts at:      0     184     438 
   Size:        184     254
 
StyI C'CwwG_G

Cuts at:      0     342     438 
   Size:        342      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     297     438 
   Size:        297     141
 
Tth111I GACn'n_nGTC

Cuts at:      0     213     438 
   Size:        213     225
 
XmnI GAAnn'nnTTC

Cuts at:      0     241     438 
   Size:        241     197
 

 Enzymes that do cut:

     AclI     AvaI    AvrII     BanI    BanII     BbsI Bme1580I   Bpu10I
    BsaHI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BssSI
    BstXI     EarI     EciI  EcoICRI     MslI   MspA1I     NspI    PflMI
    PvuII     SacI     StyI    TaqII  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI