Restriction map for
pF1KB9621
(Linear) (Six-base) MAPSORT of: KIBB9621.seq Check: 3638 from: 1 to: 2052
KIBB9621.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AatII G_ACGT'C
Cuts at: 0 20 501 2052
Size: 20 481 1551
Fragments arranged by size:
1551 481 20
AccI GT'mk_AC
Cuts at: 0 377 1607 2052
Size: 377 1230 445
Fragments arranged by size:
1230 445 377
Acc65I G'GTAC_C
Cuts at: 0 1795 2052
Size: 1795 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 2052
Size: 833 1219
AhdI GACnn_n'nnGTC
Cuts at: 0 357 1962 2052
Size: 357 1605 90
Fragments arranged by size:
1605 357 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1268 2052
Size: 1268 784
AlwNI CAG_nnn'CTG
Cuts at: 0 429 666 738 1011 2052
Size: 429 237 72 273 1041
Fragments arranged by size:
1041 429 273 237 72
ApaI G_GGCC'C
Cuts at: 0 894 2052
Size: 894 1158
AseI AT'TA_AT
Cuts at: 0 1787 2052
Size: 1787 265
AvaI C'yCGr_G
Cuts at: 0 8 1086 2052
Size: 8 1078 966
Fragments arranged by size:
1078 966 8
BanI G'GyrC_C
Cuts at: 0 933 1795 2052
Size: 933 862 257
Fragments arranged by size:
933 862 257
BanII G_rGCy'C
Cuts at: 0 894 2052
Size: 894 1158
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 774 1551 2052
Size: 20 754 777 501
Fragments arranged by size:
777 754 501 20
BbvCI CC'TCA_GC
Cuts at: 0 1152 2018 2052
Size: 1152 866 34
Fragments arranged by size:
1152 866 34
BglI GCCn_nnn'nGGC
Cuts at: 0 148 2052
Size: 148 1904
BglII A'GATC_T
Cuts at: 0 645 2052
Size: 645 1407
BlpI GC'TnA_GC
Cuts at: 0 607 1196 2052
Size: 607 589 856
Fragments arranged by size:
856 607 589
Bme1580I G_kGCm'C
Cuts at: 0 894 2052
Size: 894 1158
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 761 1021 1588 2052
Size: 570 191 260 567 464
Fragments arranged by size:
570 567 464 260 191
BmtI G_CTAG'C
Cuts at: 0 268 2052
Size: 268 1784
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1974 2052
Size: 1974 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1361 2052
Size: 760 601 691
Fragments arranged by size:
760 691 601
Bpu10I CC'TnA_GC
Cuts at: 0 1152 2018 2052
Size: 1152 866 34
Fragments arranged by size:
1152 866 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 514 2052
Size: 142 372 1538
Fragments arranged by size:
1538 372 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 1322 1657 2052
Size: 1322 335 395
Fragments arranged by size:
1322 395 335
BsaBI GATnn'nnATC
Cuts at: 0 1036 2052
Size: 1036 1016
BsaHI Gr'CG_yC
Cuts at: 0 17 211 353 498 542 2052
Size: 17 194 142 145 44 1510
Fragments arranged by size:
1510 194 145 142 44 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 2052
Size: 424 30 1598
Fragments arranged by size:
1598 424 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 646 1140 2052
Size: 127 519 494 912
Fragments arranged by size:
912 519 494 127
BseYI C'CCAG_C
Cuts at: 0 659 1614 2052
Size: 659 955 438
Fragments arranged by size:
955 659 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 501 751 2052
Size: 442 59 250 1301
Fragments arranged by size:
1301 442 250 59
BsiHKAI G_wGCw'C
Cuts at: 0 1848 2052
Size: 1848 204
BsmI GAATG_Cn'
Cuts at: 0 1369 2052
Size: 1369 683
Bsp1286I G_dGCh'C
Cuts at: 0 894 1848 2052
Size: 894 954 204
Fragments arranged by size:
954 894 204
BspHI T'CATG_A
Cuts at: 0 1598 1689 2052
Size: 1598 91 363
Fragments arranged by size:
1598 363 91
BsrBI CCG'CTC
Cuts at: 0 708 759 2052
Size: 708 51 1293
Fragments arranged by size:
1293 708 51
BsrGI T'GTAC_A
Cuts at: 0 920 2052
Size: 920 1132
BssSI C'ACGA_G
Cuts at: 0 1847 2052
Size: 1847 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1484 2052
Size: 1484 568
BstEII G'GTnAC_C
Cuts at: 0 910 2052
Size: 910 1142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 1032 2052
Size: 204 828 1020
Fragments arranged by size:
1020 828 204
BstYI r'GATC_y
Cuts at: 0 645 1059 2052
Size: 645 414 993
Fragments arranged by size:
993 645 414
BtgI C'CryG_G
Cuts at: 0 48 2052
Size: 48 2004
BtsI GCAGTG_nn'
Cuts at: 0 739 2052
Size: 739 1313
DraIII CAC_nnn'GTG
Cuts at: 0 348 1912 2052
Size: 348 1564 140
Fragments arranged by size:
1564 348 140
DrdI GACnn_nn'nnGTC
Cuts at: 0 1594 2052
Size: 1594 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1650 2052
Size: 1650 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 833 1361 2052
Size: 760 73 528 691
Fragments arranged by size:
760 691 528 73
EcoO109I rG'GnC_Cy
Cuts at: 0 763 890 891 2052
Size: 763 127 1 1161
Fragments arranged by size:
1161 763 127 1
HaeII r_GCGC'y
Cuts at: 0 429 2052
Size: 429 1623
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1069 1101 1671 1703 1948 1980
Size: 343 32 694 32 570 32 245 32
Cuts at: 1980 2052
Size: 72
Fragments arranged by size:
694 570 343 245 72 32 32 32
32
HincII GTy'rAC
Cuts at: 0 378 1467 2052
Size: 378 1089 585
Fragments arranged by size:
1089 585 378
HindIII A'AGCT_T
Cuts at: 0 559 2052
Size: 559 1493
KpnI G_GTAC'C
Cuts at: 0 1799 2052
Size: 1799 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 2052
Size: 467 1585
MslI CAynn'nnrTG
Cuts at: 0 202 1285 2052
Size: 202 1083 767
Fragments arranged by size:
1083 767 202
MspA1I CmG'CkG
Cuts at: 0 221 271 456 663 1156 2052
Size: 221 50 185 207 493 896
Fragments arranged by size:
896 493 221 207 185 50
NheI G'CTAG_C
Cuts at: 0 264 2052
Size: 264 1788
NspI r_CATG'y
Cuts at: 0 297 312 450 1482 1556 2052
Size: 297 15 138 1032 74 496
Fragments arranged by size:
1032 496 297 138 74 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 1011 2052
Size: 666 345 1041
Fragments arranged by size:
1041 666 345
PpuMI rG'GwC_Cy
Cuts at: 0 763 2052
Size: 763 1289
PspOMI G'GGCC_C
Cuts at: 0 890 2052
Size: 890 1162
PstI C_TGCA'G
Cuts at: 0 141 777 899 990 2052
Size: 141 636 122 91 1062
Fragments arranged by size:
1062 636 141 122 91
PvuII CAG'CTG
Cuts at: 0 271 456 663 1156 2052
Size: 271 185 207 493 896
Fragments arranged by size:
896 493 271 207 185
SalI G'TCGA_C
Cuts at: 0 376 2052
Size: 376 1676
SanDI GG'GwC_CC
Cuts at: 0 763 2052
Size: 763 1289
ScaI AGT'ACT
Cuts at: 0 868 1923 2052
Size: 868 1055 129
Fragments arranged by size:
1055 868 129
SexAI A'CCwGG_T
Cuts at: 0 1872 2052
Size: 1872 180
SfcI C'TryA_G
Cuts at: 0 137 451 773 895 986 1368 2052
Size: 137 314 322 122 91 382 684
Fragments arranged by size:
684 382 322 314 137 122 91
SmaI CCC'GGG
Cuts at: 0 10 2052
Size: 10 2042
SmlI C'TyrA_G
Cuts at: 0 157 529 2052
Size: 157 372 1523
Fragments arranged by size:
1523 372 157
SphI G_CATG'C
Cuts at: 0 1482 2052
Size: 1482 570
SspI AAT'ATT
Cuts at: 0 1647 2052
Size: 1647 405
StuI AGG'CCT
Cuts at: 0 1808 2052
Size: 1808 244
StyI C'CwwG_G
Cuts at: 0 1025 2052
Size: 1025 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1206 1992 2052
Size: 1206 786 60
Fragments arranged by size:
1206 786 60
TatI w'GTAC_w
Cuts at: 0 866 920 1921 2052
Size: 866 54 1001 131
Fragments arranged by size:
1001 866 131 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 2052
Size: 1765 32 255
Fragments arranged by size:
1765 255 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 2052
Size: 345 1707
XmaI C'CCGG_G
Cuts at: 0 8 2052
Size: 8 2044
XmnI GAAnn'nnTTC
Cuts at: 0 618 2052
Size: 618 1434
ZraI GAC'GTC
Cuts at: 0 18 499 2052
Size: 18 481 1553
Fragments arranged by size:
1553 481 18
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AlfI AlwNI ApaI
AseI AvaI BanI BanII BbsI BbvCI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrGI BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI DraIII DrdI EciI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII KpnI MmeI MslI
MspA1I NheI NspI PflMI PpuMI PspOMI PstI PvuII
SalI SanDI ScaI SexAI SfcI SmaI SmlI SphI
SspI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BmgBI BsaAI BsaWI BsiEI
BsiWI BsmBI BspEI BspMI BsrDI BsrFI BssHII BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I
XbaI XhoI