Restriction map for
pF1KB9620
(Linear) (Six-base) MAPSORT of: KIBB9620.seq Check: 8754 from: 1 to: 1041
KIBB9620.orf
With 182 enzymes: SgfI *
September 28, 2010 17:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 159 948 1041
Size: 159 789 93
Fragments arranged by size:
789 159 93
AccI GT'mk_AC
Cuts at: 0 578 1041
Size: 578 463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1041
Size: 307 734
AfeI AGC'GCT
Cuts at: 0 182 1041
Size: 182 859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 1041
Size: 841 200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 1041
Size: 388 32 621
Fragments arranged by size:
621 388 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1004 1014 1041
Size: 1004 10 27
Fragments arranged by size:
1004 27 10
ApaI G_GGCC'C
Cuts at: 0 506 1041
Size: 506 535
AvaI C'yCGr_G
Cuts at: 0 81 97 301 307 507 1041
Size: 81 16 204 6 200 534
Fragments arranged by size:
534 204 200 81 16 6
BanI G'GyrC_C
Cuts at: 0 49 102 229 243 607 625 735 1029
Size: 49 53 127 14 364 18 110 294
Cuts at: 1029 1041
Size: 12
Fragments arranged by size:
364 294 127 110 53 49 18 14
12
BanII G_rGCy'C
Cuts at: 0 308 361 506 526 766 1041
Size: 308 53 145 20 240 275
Fragments arranged by size:
308 275 240 145 53 20
BbeI G_GCGC'C
Cuts at: 0 53 106 233 611 629 1041
Size: 53 53 127 378 18 412
Fragments arranged by size:
412 378 127 53 53 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 907 1041
Size: 907 134
BbvCI CC'TCA_GC
Cuts at: 0 985 1041
Size: 985 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 930 964 1041
Size: 819 34 77 34 77
Fragments arranged by size:
819 77 77 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 553 1041
Size: 44 509 488
Fragments arranged by size:
509 488 44
BlpI GC'TnA_GC
Cuts at: 0 31 154 1041
Size: 31 123 887
Fragments arranged by size:
887 123 31
Bme1580I G_kGCm'C
Cuts at: 0 506 514 662 740 1034 1041
Size: 506 8 148 78 294 7
Fragments arranged by size:
506 294 148 78 8 7
BmgBI CAC'GTC
Cuts at: 0 744 993 1041
Size: 744 249 48
Fragments arranged by size:
744 249 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 894 1041
Size: 894 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 889 1041
Size: 451 438 152
Fragments arranged by size:
451 438 152
Bpu10I CC'TnA_GC
Cuts at: 0 589 985 1041
Size: 589 396 56
Fragments arranged by size:
589 396 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 700 892 955 1041
Size: 169 531 192 63 86
Fragments arranged by size:
531 192 169 86 63
BsaHI Gr'CG_yC
Cuts at: 0 50 103 230 608 626 931 1041
Size: 50 53 127 378 18 305 110
Fragments arranged by size:
378 305 127 110 53 50 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 916 946 1041
Size: 388 30 498 30 95
Fragments arranged by size:
498 388 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 1041
Size: 409 632
BseYI C'CCAG_C
Cuts at: 0 235 1019 1041
Size: 235 784 22
Fragments arranged by size:
784 235 22
BsiEI CG_ry'CG
Cuts at: 0 116 122 197 473 560 638 1041
Size: 116 6 75 276 87 78 403
Fragments arranged by size:
403 276 116 87 78 75 6
BsiHKAI G_wGCw'C
Cuts at: 0 308 361 1041
Size: 308 53 680
Fragments arranged by size:
680 308 53
Bsp1286I G_dGCh'C
Cuts at: 0 308 361 506 514 526 662 740 766
Size: 308 53 145 8 12 136 78 26
Cuts at: 766 1034 1041
Size: 268 7
Fragments arranged by size:
308 268 145 136 78 53 26 12
8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 159 948 1041
Size: 159 789 93
Fragments arranged by size:
789 159 93
BsrBI CCG'CTC
Cuts at: 0 35 1041
Size: 35 1006
BsrFI r'CCGG_y
Cuts at: 0 121 1041
Size: 121 920
BssHII G'CGCG_C
Cuts at: 0 219 1041
Size: 219 822
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1041
Size: 1029 12
BstEII G'GTnAC_C
Cuts at: 0 339 1041
Size: 339 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 786 1041
Size: 336 450 255
Fragments arranged by size:
450 336 255
BtgI C'CryG_G
Cuts at: 0 116 518 935 1041
Size: 116 402 417 106
Fragments arranged by size:
417 402 116 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 822 1041
Size: 35 787 219
Fragments arranged by size:
787 219 35
DraIII CAC_nnn'GTG
Cuts at: 0 1029 1041
Size: 1029 12
EaeI y'GGCC_r
Cuts at: 0 113 119 194 386 470 515 557 1041
Size: 113 6 75 192 84 45 42 484
Fragments arranged by size:
484 192 113 84 75 45 42 6
EagI C'GGCC_G
Cuts at: 0 113 119 194 470 557 1041
Size: 113 6 75 276 87 484
Fragments arranged by size:
484 276 113 87 75 6
EarI CTCTTCn'nnn_
Cuts at: 0 884 1041
Size: 884 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 826 1041
Size: 826 215
EcoICRI GAG'CTC
Cuts at: 0 306 359 1041
Size: 306 53 682
Fragments arranged by size:
682 306 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 451 889 1041
Size: 307 144 438 152
Fragments arranged by size:
438 307 152 144
EcoO109I rG'GnC_Cy
Cuts at: 0 426 502 503 1041
Size: 426 76 1 538
Fragments arranged by size:
538 426 76 1
FspI TGC'GCA
Cuts at: 0 829 1041
Size: 829 212
HaeII r_GCGC'y
Cuts at: 0 31 53 106 184 233 315 574 611
Size: 31 22 53 78 49 82 259 37
Cuts at: 611 629 1041
Size: 18 412
Fragments arranged by size:
412 259 82 78 53 49 37 31
22 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1041
Size: 916 32 93
Fragments arranged by size:
916 93 32
HincII GTy'rAC
Cuts at: 0 996 1041
Size: 996 45
KasI G'GCGC_C
Cuts at: 0 49 102 229 607 625 1041
Size: 49 53 127 378 18 416
Fragments arranged by size:
416 378 127 53 49 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1041
Size: 353 688
MslI CAynn'nnrTG
Cuts at: 0 784 1041
Size: 784 257
MspA1I CmG'CkG
Cuts at: 0 62 118 475 1001 1011 1041
Size: 62 56 357 526 10 30
Fragments arranged by size:
526 357 62 56 30 10
NaeI GCC'GGC
Cuts at: 0 123 1041
Size: 123 918
NarI GG'CG_CC
Cuts at: 0 50 103 230 608 626 1041
Size: 50 53 127 378 18 415
Fragments arranged by size:
415 378 127 53 50 18
NgoMIV G'CCGG_C
Cuts at: 0 121 1041
Size: 121 920
NotI GC'GGCC_GC
Cuts at: 0 194 470 557 1041
Size: 194 276 87 484
Fragments arranged by size:
484 276 194 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 388 420 1041
Size: 388 32 621
Fragments arranged by size:
621 388 32
PspOMI G'GGCC_C
Cuts at: 0 502 1041
Size: 502 539
PspXI vC'TCGA_Gb
Cuts at: 0 307 1041
Size: 307 734
PvuII CAG'CTG
Cuts at: 0 1011 1041
Size: 1011 30
SacI G_AGCT'C
Cuts at: 0 308 361 1041
Size: 308 53 680
Fragments arranged by size:
680 308 53
SacII CC_GC'GG
Cuts at: 0 119 1041
Size: 119 922
SexAI A'CCwGG_T
Cuts at: 0 1025 1041
Size: 1025 16
SfoI GGC'GCC
Cuts at: 0 51 104 231 609 627 1041
Size: 51 53 127 378 18 414
Fragments arranged by size:
414 378 127 53 51 18
SmaI CCC'GGG
Cuts at: 0 83 99 1041
Size: 83 16 942
Fragments arranged by size:
942 83 16
SmlI C'TyrA_G
Cuts at: 0 184 307 715 907 970 1041
Size: 184 123 408 192 63 71
Fragments arranged by size:
408 192 184 123 71 63
StyI C'CwwG_G
Cuts at: 0 377 1041
Size: 377 664
XhoI C'TCGA_G
Cuts at: 0 307 1041
Size: 307 734
XmaI C'CCGG_G
Cuts at: 0 81 97 1041
Size: 81 16 944
Fragments arranged by size:
944 81 16
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AloI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BtgI BtgZI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NotI PpiI PspOMI PspXI PvuII SacI
SacII SexAI SfoI SmaI SmlI StyI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BmrI BmtI BsaI BsaAI
BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmnI ZraI