Restriction map for
pF1KB9615
(Linear) (Six-base) MAPSORT of: KIBB9615.seq Check: 2317 from: 1 to: 792
KIBB9615.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 192 476 792
Size: 192 284 316
Fragments arranged by size:
316 284 192
Acc65I G'GTAC_C
Cuts at: 0 678 792
Size: 678 114
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 436 792
Size: 311 125 356
Fragments arranged by size:
356 311 125
AfeI AGC'GCT
Cuts at: 0 457 792
Size: 457 335
AlwNI CAG_nnn'CTG
Cuts at: 0 304 706 792
Size: 304 402 86
Fragments arranged by size:
402 304 86
ApaLI G'TGCA_C
Cuts at: 0 571 689 792
Size: 571 118 103
Fragments arranged by size:
571 118 103
AvaI C'yCGr_G
Cuts at: 0 40 83 576 792
Size: 40 43 493 216
Fragments arranged by size:
493 216 43 40
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 670 703 792
Size: 670 33 89
Fragments arranged by size:
670 89 33
BanI G'GyrC_C
Cuts at: 0 641 678 792
Size: 641 37 114
Fragments arranged by size:
641 114 37
BanII G_rGCy'C
Cuts at: 0 191 319 792
Size: 191 128 473
Fragments arranged by size:
473 191 128
BbeI G_GCGC'C
Cuts at: 0 645 792
Size: 645 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 581 792
Size: 547 34 211
Fragments arranged by size:
547 211 34
BclI T'GATC_A
Cuts at: 0 264 792
Size: 264 528
BglI GCCn_nnn'nGGC
Cuts at: 0 95 792
Size: 95 697
Bme1580I G_kGCm'C
Cuts at: 0 278 526 575 601 693 792
Size: 278 248 49 26 92 99
Fragments arranged by size:
278 248 99 92 49 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 792
Size: 217 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 444 792
Size: 406 38 348
Fragments arranged by size:
406 348 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 792
Size: 121 671
BsaI GGTCTCn'nnnn_
Cuts at: 0 291 792
Size: 291 501
BsaHI Gr'CG_yC
Cuts at: 0 642 792
Size: 642 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 792
Size: 574 30 188
Fragments arranged by size:
574 188 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 62 111 241 739 775 778 792
Size: 62 49 130 498 36 3 14
Fragments arranged by size:
498 130 62 49 36 14 3
BseYI C'CCAG_C
Cuts at: 0 10 304 792
Size: 10 294 488
Fragments arranged by size:
488 294 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 226 792
Size: 220 6 566
Fragments arranged by size:
566 220 6
BsiHKAI G_wGCw'C
Cuts at: 0 575 693 792
Size: 575 118 99
Fragments arranged by size:
575 118 99
Bsp1286I G_dGCh'C
Cuts at: 0 191 278 319 526 575 601 693 792
Size: 191 87 41 207 49 26 92 99
Fragments arranged by size:
207 191 99 92 87 49 41 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 192 476 792
Size: 192 284 316
Fragments arranged by size:
316 284 192
BsrFI r'CCGG_y
Cuts at: 0 493 786 792
Size: 493 293 6
Fragments arranged by size:
493 293 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 437 792
Size: 437 355
BstYI r'GATC_y
Cuts at: 0 696 792
Size: 696 96
Bsu36I CC'TnA_GG
Cuts at: 0 311 792
Size: 311 481
BtgI C'CryG_G
Cuts at: 0 671 715 792
Size: 671 44 77
Fragments arranged by size:
671 77 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 550 792
Size: 550 242
BtsI GCAGTG_nn'
Cuts at: 0 240 477 792
Size: 240 237 315
Fragments arranged by size:
315 240 237
ClaI AT'CG_AT
Cuts at: 0 260 792
Size: 260 532
EarI CTCTTCn'nnn_
Cuts at: 0 44 752 792
Size: 44 708 40
Fragments arranged by size:
708 44 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 406 436 444 792
Size: 311 95 30 8 348
Fragments arranged by size:
348 311 95 30 8
EcoO109I rG'GnC_Cy
Cuts at: 0 333 792
Size: 333 459
HaeII r_GCGC'y
Cuts at: 0 393 459 588 645 792
Size: 393 66 129 57 147
Fragments arranged by size:
393 147 129 66 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 688 720 792
Size: 249 32 407 32 72
Fragments arranged by size:
407 249 72 32 32
KasI G'GCGC_C
Cuts at: 0 641 792
Size: 641 151
KpnI G_GTAC'C
Cuts at: 0 682 792
Size: 682 110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 792
Size: 404 388
MspA1I CmG'CkG
Cuts at: 0 301 512 519 792
Size: 301 211 7 273
Fragments arranged by size:
301 273 211 7
NaeI GCC'GGC
Cuts at: 0 495 788 792
Size: 495 293 4
Fragments arranged by size:
495 293 4
NarI GG'CG_CC
Cuts at: 0 642 792
Size: 642 150
NcoI C'CATG_G
Cuts at: 0 671 792
Size: 671 121
NgoMIV G'CCGG_C
Cuts at: 0 493 786 792
Size: 493 293 6
Fragments arranged by size:
493 293 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 95 792
Size: 95 697
SfoI GGC'GCC
Cuts at: 0 643 792
Size: 643 149
SmaI CCC'GGG
Cuts at: 0 42 85 792
Size: 42 43 707
Fragments arranged by size:
707 43 42
SmlI C'TyrA_G
Cuts at: 0 136 792
Size: 136 656
StuI AGG'CCT
Cuts at: 0 142 792
Size: 142 650
StyI C'CwwG_G
Cuts at: 0 671 792
Size: 671 121
XmaI C'CCGG_G
Cuts at: 0 40 83 792
Size: 40 43 709
Fragments arranged by size:
709 43 40
Enzymes that do cut:
AarI Acc65I AcuI AfeI AlwNI ApaLI AvaI BaeI
BanI BanII BbeI BcgI BclI BglI Bme1580I BmrI
BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI ClaI EarI Eco57MI EcoO109I HaeII Hin4I
KasI KpnI MmeI MspA1I NaeI NarI NcoI NgoMIV
SfiI SfoI SmaI SmlI StuI StyI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BamHI BbsI BbvCI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MscI MslI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI