Restriction map for
pF1KB9614
(Linear) (Six-base) MAPSORT of: KIBB9614.seq Check: 8244 from: 1 to: 1506
KIBB9614.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 684 848 1104 1506
Size: 65 619 164 256 402
Fragments arranged by size:
619 402 256 164 65
AflIII A'CryG_T
Cuts at: 0 134 424 1506
Size: 134 290 1082
Fragments arranged by size:
1082 290 134
AhdI GACnn_n'nnGTC
Cuts at: 0 1457 1506
Size: 1457 49
ApoI r'AATT_y
Cuts at: 0 38 79 566 650 708 818 902 986
Size: 38 41 487 84 58 110 84 84
Cuts at: 986 1070 1154 1238 1322 1406 1506
Size: 84 84 84 84 84 100
Fragments arranged by size:
487 110 100 84 84 84 84 84
84 84 84 58 41 38
BanI G'GyrC_C
Cuts at: 0 557 1506
Size: 557 949
BanII G_rGCy'C
Cuts at: 0 1468 1506
Size: 1468 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 137 784 1456 1506
Size: 137 647 672 50
Fragments arranged by size:
672 647 137 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 680 764 932 1184 1268 1436 1506
Size: 596 84 84 168 252 84 168 70
Fragments arranged by size:
596 252 168 168 84 84 84 70
Bpu10I CC'TnA_GC
Cuts at: 0 879 1506
Size: 879 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1506
Size: 225 1281
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 1506
Size: 660 846
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 179 1465 1506
Size: 179 1286 41
Fragments arranged by size:
1286 179 41
BseYI C'CCAG_C
Cuts at: 0 289 1506
Size: 289 1217
BsiHKAI G_wGCw'C
Cuts at: 0 1468 1506
Size: 1468 38
BsmI GAATG_Cn'
Cuts at: 0 850 1186 1380 1506
Size: 850 336 194 126
Fragments arranged by size:
850 336 194 126
Bsp1286I G_dGCh'C
Cuts at: 0 1468 1506
Size: 1468 38
BspHI T'CATG_A
Cuts at: 0 105 1506
Size: 105 1401
BsrDI GCAATG_nn'
Cuts at: 0 855 1506
Size: 855 651
Bsu36I CC'TnA_GG
Cuts at: 0 191 1506
Size: 191 1315
BtgI C'CryG_G
Cuts at: 0 161 1506
Size: 161 1345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 837 872 1506
Size: 837 35 634
Fragments arranged by size:
837 634 35
EaeI y'GGCC_r
Cuts at: 0 354 1038 1506
Size: 354 684 468
Fragments arranged by size:
684 468 354
EarI CTCTTCn'nnn_
Cuts at: 0 161 1506
Size: 161 1345
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1224 1506
Size: 1224 282
EcoICRI GAG'CTC
Cuts at: 0 1466 1506
Size: 1466 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 596 680 684 764 848 932 1104
Size: 65 531 84 4 80 84 84 172
Cuts at: 1104 1184 1268 1436 1506
Size: 80 84 168 70
Fragments arranged by size:
531 172 168 84 84 84 84 80
80 70 65 4
EcoRI G'AATT_C
Cuts at: 0 566 650 818 986 1070 1154 1238 1406
Size: 566 84 168 168 84 84 84 168
Cuts at: 1406 1506
Size: 100
Fragments arranged by size:
566 168 168 168 100 84 84 84
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 174 193 201 206 225 1443 1475
Size: 169 5 19 8 5 19 1218 32
Cuts at: 1475 1506
Size: 31
Fragments arranged by size:
1218 169 32 31 19 19 8 5
5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1441 1503 1506
Size: 119 1322 62 3
Fragments arranged by size:
1322 119 62 3
MscI TGG'CCA
Cuts at: 0 356 1040 1506
Size: 356 684 466
Fragments arranged by size:
684 466 356
MslI CAynn'nnrTG
Cuts at: 0 759 927 1079 1487 1506
Size: 759 168 152 408 19
Fragments arranged by size:
759 408 168 152 19
NcoI C'CATG_G
Cuts at: 0 161 1506
Size: 161 1345
NdeI CA'TA_TG
Cuts at: 0 476 756 1506
Size: 476 280 750
Fragments arranged by size:
750 476 280
NspI r_CATG'y
Cuts at: 0 138 428 1506
Size: 138 290 1078
Fragments arranged by size:
1078 290 138
PciI A'CATG_T
Cuts at: 0 134 424 1506
Size: 134 290 1082
Fragments arranged by size:
1082 290 134
PstI C_TGCA'G
Cuts at: 0 286 1506
Size: 286 1220
SacI G_AGCT'C
Cuts at: 0 1468 1506
Size: 1468 38
SfcI C'TryA_G
Cuts at: 0 282 1506
Size: 282 1224
SmlI C'TyrA_G
Cuts at: 0 240 1506
Size: 240 1266
StuI AGG'CCT
Cuts at: 0 613 884 1117 1506
Size: 613 271 233 389
Fragments arranged by size:
613 389 271 233
StyI C'CwwG_G
Cuts at: 0 29 99 161 1506
Size: 29 70 62 1345
Fragments arranged by size:
1345 70 62 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1506
Size: 452 32 1022
Fragments arranged by size:
1022 452 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 297 1506
Size: 297 1209
XmnI GAAnn'nnTTC
Cuts at: 0 80 540 949 1117 1506
Size: 80 460 409 168 389
Fragments arranged by size:
460 409 389 168 80
Enzymes that do cut:
AcuI AflIII AhdI ApoI BanI BanII BbsI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BsrDI Bsu36I BtgI CspCI EaeI EarI EciI
EcoICRI Eco57MI EcoRI Hin4I MmeI MscI MslI NcoI
NdeI NspI PciI PstI SacI SfcI SmlI StuI
StyI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI