Restriction map for
pF1KB9612
(Linear) (Six-base) MAPSORT of: KIBB9612.seq Check: 9863 from: 1 to: 2073
KIBB9612.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 715 1354 2073
Size: 298 417 639 719
Fragments arranged by size:
719 639 417 298
AflII C'TTAA_G
Cuts at: 0 1027 2073
Size: 1027 1046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1617 2073
Size: 1617 456
ApoI r'AATT_y
Cuts at: 0 962 1251 1345 1612 2073
Size: 962 289 94 267 461
Fragments arranged by size:
962 461 289 267 94
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1357 1390 2073
Size: 1357 33 683
Fragments arranged by size:
1357 683 33
BanII G_rGCy'C
Cuts at: 0 738 1132 2073
Size: 738 394 941
Fragments arranged by size:
941 738 394
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 1780 1857 2073
Size: 286 1494 77 216
Fragments arranged by size:
1494 286 216 77
BfrBI ATG'CAT
Cuts at: 0 481 1594 2073
Size: 481 1113 479
Fragments arranged by size:
1113 481 479
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1146 2073
Size: 1146 927
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 373 2073
Size: 229 144 1700
Fragments arranged by size:
1700 229 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1116 2073
Size: 1116 957
BsaI GGTCTCn'nnnn_
Cuts at: 0 1716 2073
Size: 1716 357
BsaAI yAC'GTr
Cuts at: 0 1405 2073
Size: 1405 668
BsaBI GATnn'nnATC
Cuts at: 0 1908 2073
Size: 1908 165
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 1985 2073
Size: 850 1135 88
Fragments arranged by size:
1135 850 88
BsiHKAI G_wGCw'C
Cuts at: 0 433 1132 2073
Size: 433 699 941
Fragments arranged by size:
941 699 433
BsmI GAATG_Cn'
Cuts at: 0 1966 2073
Size: 1966 107
Bsp1286I G_dGCh'C
Cuts at: 0 433 738 1132 2073
Size: 433 305 394 941
Fragments arranged by size:
941 433 394 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 962 2073
Size: 962 1111
BsrGI T'GTAC_A
Cuts at: 0 484 2073
Size: 484 1589
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1232 2073
Size: 1232 841
BtsI GCAGTG_nn'
Cuts at: 0 1887 2073
Size: 1887 186
DraI TTT'AAA
Cuts at: 0 20 243 270 564 1083 2028 2073
Size: 20 223 27 294 519 945 45
Fragments arranged by size:
945 519 294 223 45 27 20
EaeI y'GGCC_r
Cuts at: 0 1019 2073
Size: 1019 1054
EarI CTCTTCn'nnn_
Cuts at: 0 745 1999 2073
Size: 745 1254 74
Fragments arranged by size:
1254 745 74
EcoICRI GAG'CTC
Cuts at: 0 1130 2073
Size: 1130 943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 298 373 715 1354 2073
Size: 229 69 75 342 639 719
Fragments arranged by size:
719 639 342 229 75 69
EcoO109I rG'GnC_Cy
Cuts at: 0 1502 2073
Size: 1502 571
EcoRI G'AATT_C
Cuts at: 0 1345 2073
Size: 1345 728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1110 1282 1332 1841 2073
Size: 1110 172 50 509 232
Fragments arranged by size:
1110 509 232 172 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 1714 1746 2073
Size: 844 32 838 32 327
Fragments arranged by size:
844 838 327 32 32
HincII GTy'rAC
Cuts at: 0 599 1659 2073
Size: 599 1060 414
Fragments arranged by size:
1060 599 414
HindIII A'AGCT_T
Cuts at: 0 1024 2073
Size: 1024 1049
HpaI GTT'AAC
Cuts at: 0 1659 2073
Size: 1659 414
MfeI C'AATT_G
Cuts at: 0 1190 2073
Size: 1190 883
MscI TGG'CCA
Cuts at: 0 1021 2073
Size: 1021 1052
MslI CAynn'nnrTG
Cuts at: 0 165 1959 2073
Size: 165 1794 114
Fragments arranged by size:
1794 165 114
MspA1I CmG'CkG
Cuts at: 0 938 2073
Size: 938 1135
NdeI CA'TA_TG
Cuts at: 0 78 2073
Size: 78 1995
NsiI A_TGCA'T
Cuts at: 0 483 1596 2073
Size: 483 1113 477
Fragments arranged by size:
1113 483 477
NspI r_CATG'y
Cuts at: 0 485 535 2073
Size: 485 50 1538
Fragments arranged by size:
1538 485 50
PvuII CAG'CTG
Cuts at: 0 938 2073
Size: 938 1135
SacI G_AGCT'C
Cuts at: 0 1132 2073
Size: 1132 941
SmlI C'TyrA_G
Cuts at: 0 1027 1095 2073
Size: 1027 68 978
Fragments arranged by size:
1027 978 68
SnaBI TAC'GTA
Cuts at: 0 1405 2073
Size: 1405 668
SpeI A'CTAG_T
Cuts at: 0 198 2073
Size: 198 1875
SspI AAT'ATT
Cuts at: 0 90 474 1518 2073
Size: 90 384 1044 555
Fragments arranged by size:
1044 555 384 90
StuI AGG'CCT
Cuts at: 0 558 2073
Size: 558 1515
StyI C'CwwG_G
Cuts at: 0 1390 2073
Size: 1390 683
TatI w'GTAC_w
Cuts at: 0 484 534 577 1797 2073
Size: 484 50 43 1220 276
Fragments arranged by size:
1220 484 276 50 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 2073
Size: 839 32 1202
Fragments arranged by size:
1202 839 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1612 2073
Size: 1612 461
Enzymes that do cut:
AcuI AflII AlfI ApoI BaeI BanII BbsI BfrBI
BplI BpmI BpuEI BsaI BsaAI BsaBI BseRI BsiHKAI
BsmI Bsp1286I BspMI BsrGI BstXI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII
HindIII HpaI MfeI MscI MslI MspA1I NdeI NsiI
NspI PvuII SacI SmlI SnaBI SpeI SspI StuI
StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI Bpu10I BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI
EcoRV FseI FspI FspAI HaeII KasI KpnI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SphI SrfI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI