Restriction map for
pF1KB9609
(Linear) (Six-base) MAPSORT of: KIBB9609.seq Check: 6116 from: 1 to: 723
KIBB9609.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 396 480 723
Size: 60 336 84 243
Fragments arranged by size:
336 243 84 60
AhdI GACnn_n'nnGTC
Cuts at: 0 329 723
Size: 329 394
ApaLI G'TGCA_C
Cuts at: 0 247 723
Size: 247 476
ApoI r'AATT_y
Cuts at: 0 110 278 362 614 712 723
Size: 110 168 84 252 98 11
Fragments arranged by size:
252 168 110 98 84 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 723
Size: 248 475
Bme1580I G_kGCm'C
Cuts at: 0 174 251 723
Size: 174 77 472
Fragments arranged by size:
472 174 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 308 723
Size: 224 84 415
Fragments arranged by size:
415 224 84
BseYI C'CCAG_C
Cuts at: 0 173 723
Size: 173 550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 663 723
Size: 337 326 60
Fragments arranged by size:
337 326 60
BsiHKAI G_wGCw'C
Cuts at: 0 251 723
Size: 251 472
BsmI GAATG_Cn'
Cuts at: 0 58 394 723
Size: 58 336 329
Fragments arranged by size:
336 329 58
Bsp1286I G_dGCh'C
Cuts at: 0 174 251 723
Size: 174 77 472
Fragments arranged by size:
472 174 77
BstAPI GCAn_nnn'nTGC
Cuts at: 0 650 723
Size: 650 73
BstXI CCAn_nnnn'nTGG
Cuts at: 0 568 723
Size: 568 155
BtsI GCAGTG_nn'
Cuts at: 0 63 723
Size: 63 660
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 381 416 465 500 723
Size: 381 35 49 35 223
Fragments arranged by size:
381 223 49 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 454 538 723
Size: 454 84 185
Fragments arranged by size:
454 185 84
EarI CTCTTCn'nnn_
Cuts at: 0 147 513 723
Size: 147 366 210
Fragments arranged by size:
366 210 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 224 308 396 480 723
Size: 60 164 84 88 84 243
Fragments arranged by size:
243 164 88 84 84 60
EcoRI G'AATT_C
Cuts at: 0 110 278 362 614 723
Size: 110 168 84 252 109
Fragments arranged by size:
252 168 110 109 84
FspI TGC'GCA
Cuts at: 0 503 723
Size: 503 220
FspAI rTGC'GCAy
Cuts at: 0 503 723
Size: 503 220
HaeII r_GCGC'y
Cuts at: 0 419 723
Size: 419 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 315 347 723
Size: 315 32 376
Fragments arranged by size:
376 315 32
HincII GTy'rAC
Cuts at: 0 604 723
Size: 604 119
MslI CAynn'nnrTG
Cuts at: 0 303 371 566 623 723
Size: 303 68 195 57 100
Fragments arranged by size:
303 195 100 68 57
NspI r_CATG'y
Cuts at: 0 560 723
Size: 560 163
PstI C_TGCA'G
Cuts at: 0 673 723
Size: 673 50
SapI GCTCTTCn'nnn_
Cuts at: 0 147 723
Size: 147 576
SfcI C'TryA_G
Cuts at: 0 669 693 723
Size: 669 24 30
Fragments arranged by size:
669 30 24
StuI AGG'CCT
Cuts at: 0 73 577 723
Size: 73 504 146
Fragments arranged by size:
504 146 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 241 273 723
Size: 241 32 450
Fragments arranged by size:
450 241 32
Enzymes that do cut:
AcuI AhdI ApaLI ApoI BbsI Bme1580I BpmI BseYI
BsgI BsiHKAI BsmI Bsp1286I BstAPI BstXI BtsI CspCI
DraIII EarI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII MslI NspI PstI SapI SfcI StuI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI