Restriction map for
pF1KB9608
(Linear) (Six-base) MAPSORT of: KIBB9608.seq Check: 1706 from: 1 to: 852
KIBB9608.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 44 852
Size: 44 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 597 681 852
Size: 513 84 84 171
Fragments arranged by size:
513 171 84 84
AgeI A'CCGG_T
Cuts at: 0 755 852
Size: 755 97
AleI CACnn'nnGTG
Cuts at: 0 320 656 852
Size: 320 336 196
Fragments arranged by size:
336 320 196
ApaLI G'TGCA_C
Cuts at: 0 675 759 852
Size: 675 84 93
Fragments arranged by size:
675 93 84
AvaI C'yCGr_G
Cuts at: 0 194 363 852
Size: 194 169 489
Fragments arranged by size:
489 194 169
AvrII C'CTAG_G
Cuts at: 0 486 852
Size: 486 366
BamHI G'GATC_C
Cuts at: 0 95 311 395 852
Size: 95 216 84 457
Fragments arranged by size:
457 216 95 84
BanII G_rGCy'C
Cuts at: 0 195 793 852
Size: 195 598 59
Fragments arranged by size:
598 195 59
Bme1580I G_kGCm'C
Cuts at: 0 261 679 763 852
Size: 261 418 84 89
Fragments arranged by size:
418 261 89 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 852
Size: 255 597
BmtI G_CTAG'C
Cuts at: 0 550 852
Size: 550 302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 609 852
Size: 592 17 243
Fragments arranged by size:
592 243 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 657 852
Size: 657 195
BsaWI w'CCGG_w
Cuts at: 0 560 755 852
Size: 560 195 97
Fragments arranged by size:
560 195 97
BseYI C'CCAG_C
Cuts at: 0 835 852
Size: 835 17
BsiHKAI G_wGCw'C
Cuts at: 0 679 723 763 793 852
Size: 679 44 40 30 59
Fragments arranged by size:
679 59 44 40 30
Bsp1286I G_dGCh'C
Cuts at: 0 195 261 679 723 763 793 852
Size: 195 66 418 44 40 30 59
Fragments arranged by size:
418 195 66 59 44 40 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 852
Size: 44 808
BsrBI CCG'CTC
Cuts at: 0 393 852
Size: 393 459
BsrDI GCAATG_nn'
Cuts at: 0 212 852
Size: 212 640
BsrFI r'CCGG_y
Cuts at: 0 755 852
Size: 755 97
BssSI C'ACGA_G
Cuts at: 0 388 852
Size: 388 464
BstYI r'GATC_y
Cuts at: 0 95 311 395 852
Size: 95 216 84 457
Fragments arranged by size:
457 216 95 84
Bsu36I CC'TnA_GG
Cuts at: 0 41 852
Size: 41 811
BtgI C'CryG_G
Cuts at: 0 169 852
Size: 169 683
BtsI GCAGTG_nn'
Cuts at: 0 12 852
Size: 12 840
DraIII CAC_nnn'GTG
Cuts at: 0 683 767 852
Size: 683 84 85
Fragments arranged by size:
683 85 84
EaeI y'GGCC_r
Cuts at: 0 496 852
Size: 496 356
EarI CTCTTCn'nnn_
Cuts at: 0 401 601 852
Size: 401 200 251
Fragments arranged by size:
401 251 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 705 793 852
Size: 705 88 59
Fragments arranged by size:
705 88 59
EcoICRI GAG'CTC
Cuts at: 0 791 852
Size: 791 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 592 597 609 681 852
Size: 513 79 5 12 72 171
Fragments arranged by size:
513 171 79 72 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 44 852
Size: 40 4 808
Fragments arranged by size:
808 40 4
EcoO109I rG'GnC_Cy
Cuts at: 0 207 852
Size: 207 645
EcoRV GAT'ATC
Cuts at: 0 705 852
Size: 705 147
HaeII r_GCGC'y
Cuts at: 0 456 852
Size: 456 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 780 812 852
Size: 118 32 630 32 40
Fragments arranged by size:
630 118 40 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 314 398 734 852
Size: 116 198 84 336 118
Fragments arranged by size:
336 198 118 116 84
MslI CAynn'nnrTG
Cuts at: 0 320 656 852
Size: 320 336 196
Fragments arranged by size:
336 320 196
MspA1I CmG'CkG
Cuts at: 0 309 506 729 852
Size: 309 197 223 123
Fragments arranged by size:
309 223 197 123
NcoI C'CATG_G
Cuts at: 0 169 852
Size: 169 683
NheI G'CTAG_C
Cuts at: 0 546 852
Size: 546 306
PfoI T'CCnGG_A
Cuts at: 0 784 852
Size: 784 68
PpuMI rG'GwC_Cy
Cuts at: 0 207 852
Size: 207 645
SacI G_AGCT'C
Cuts at: 0 793 852
Size: 793 59
SanDI GG'GwC_CC
Cuts at: 0 207 852
Size: 207 645
SapI GCTCTTCn'nnn_
Cuts at: 0 601 852
Size: 601 251
SmlI C'TyrA_G
Cuts at: 0 194 363 852
Size: 194 169 489
Fragments arranged by size:
489 194 169
StyI C'CwwG_G
Cuts at: 0 64 169 223 486 852
Size: 64 105 54 263 366
Fragments arranged by size:
366 263 105 64 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 852
Size: 664 188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 167 852
Size: 167 685
XhoI C'TCGA_G
Cuts at: 0 194 363 852
Size: 194 169 489
Fragments arranged by size:
489 194 169
XmnI GAAnn'nnTTC
Cuts at: 0 610 778 852
Size: 610 168 74
Fragments arranged by size:
610 168 74
Enzymes that do cut:
AarI AcuI AgeI AleI ApaLI AvaI AvrII BamHI
BanII Bme1580I BmrI BmtI BpmI BsaI BsaWI BseYI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstYI
Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I MmeI MslI
MspA1I NcoI NheI PfoI PpuMI SacI SanDI SapI
SmlI StyI TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI
EagI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XmaI ZraI