Restriction map for
pF1KB9599
(Linear) (Six-base) MAPSORT of: KIBB9599.seq Check: 322 from: 1 to: 1317
KIBB9599.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 638 1317
Size: 156 482 679
Fragments arranged by size:
679 482 156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1317
Size: 308 1009
AflIII A'CryG_T
Cuts at: 0 557 1317
Size: 557 760
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 1317
Size: 1008 309
ApaI G_GGCC'C
Cuts at: 0 705 1313 1317
Size: 705 608 4
Fragments arranged by size:
705 608 4
AvaI C'yCGr_G
Cuts at: 0 197 225 618 726 751 1317
Size: 197 28 393 108 25 566
Fragments arranged by size:
566 393 197 108 28 25
BanI G'GyrC_C
Cuts at: 0 247 261 828 867 1160 1317
Size: 247 14 567 39 293 157
Fragments arranged by size:
567 293 247 157 39 14
BanII G_rGCy'C
Cuts at: 0 627 705 1213 1313 1317
Size: 627 78 508 100 4
Fragments arranged by size:
627 508 100 78 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 113 1317
Size: 74 39 1204
Fragments arranged by size:
1204 74 39
BbvCI CC'TCA_GC
Cuts at: 0 901 1317
Size: 901 416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 772 806 1317
Size: 772 34 511
Fragments arranged by size:
772 511 34
BfrBI ATG'CAT
Cuts at: 0 952 1317
Size: 952 365
BglI GCCn_nnn'nGGC
Cuts at: 0 515 1177 1317
Size: 515 662 140
Fragments arranged by size:
662 515 140
BlpI GC'TnA_GC
Cuts at: 0 17 379 1317
Size: 17 362 938
Fragments arranged by size:
938 362 17
Bme1580I G_kGCm'C
Cuts at: 0 264 705 831 1163 1313 1317
Size: 264 441 126 332 150 4
Fragments arranged by size:
441 332 264 150 126 4
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 1317
Size: 1272 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 552 1038 1317
Size: 136 416 486 279
Fragments arranged by size:
486 416 279 136
Bpu10I CC'TnA_GC
Cuts at: 0 901 1317
Size: 901 416
BsaHI Gr'CG_yC
Cuts at: 0 759 930 1317
Size: 759 171 387
Fragments arranged by size:
759 387 171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 1317
Size: 532 30 755
Fragments arranged by size:
755 532 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 127 130 309 779 927 1123 1317
Size: 109 18 3 179 470 148 196 194
Fragments arranged by size:
470 196 194 179 148 109 18 3
BseYI C'CCAG_C
Cuts at: 0 1152 1317
Size: 1152 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 655 760 1317
Size: 641 14 105 557
Fragments arranged by size:
641 557 105 14
BsiEI CG_ry'CG
Cuts at: 0 1030 1062 1172 1317
Size: 1030 32 110 145
Fragments arranged by size:
1030 145 110 32
BsiHKAI G_wGCw'C
Cuts at: 0 206 1317
Size: 206 1111
BsmBI CGTCTCn'nnnn_
Cuts at: 0 750 1317
Size: 750 567
Bsp1286I G_dGCh'C
Cuts at: 0 206 264 627 705 831 1163 1213 1313
Size: 206 58 363 78 126 332 50 100
Cuts at: 1313 1317
Size: 4
Fragments arranged by size:
363 332 206 126 100 78 58 50
4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 638 660 1317
Size: 156 482 22 657
Fragments arranged by size:
657 482 156 22
BsrBI CCG'CTC
Cuts at: 0 996 1317
Size: 996 321
BsrFI r'CCGG_y
Cuts at: 0 303 1317
Size: 303 1014
BstYI r'GATC_y
Cuts at: 0 491 1317
Size: 491 826
BtgI C'CryG_G
Cuts at: 0 341 1317
Size: 341 976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 312 1208 1317
Size: 312 896 109
Fragments arranged by size:
896 312 109
ClaI AT'CG_AT
Cuts at: 0 80 1317
Size: 80 1237
DrdI GACnn_nn'nnGTC
Cuts at: 0 484 1317
Size: 484 833
EaeI y'GGCC_r
Cuts at: 0 344 405 1169 1229 1295 1317
Size: 344 61 764 60 66 22
Fragments arranged by size:
764 344 66 61 60 22
EagI C'GGCC_G
Cuts at: 0 1169 1317
Size: 1169 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 445 961 1259 1317
Size: 445 516 298 58
Fragments arranged by size:
516 445 298 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 308 552 1038 1317
Size: 136 172 244 486 279
Fragments arranged by size:
486 279 244 172 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1317
Size: 91 1226
EcoO109I rG'GnC_Cy
Cuts at: 0 701 702 731 1253 1309 1310 1317
Size: 701 1 29 522 56 1 7
Fragments arranged by size:
701 522 56 29 7 1 1
FspI TGC'GCA
Cuts at: 0 1012 1317
Size: 1012 305
HaeII r_GCGC'y
Cuts at: 0 24 372 606 1183 1317
Size: 24 348 234 577 134
Fragments arranged by size:
577 348 234 134 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 191 223 532 564 1317
Size: 191 32 309 32 753
Fragments arranged by size:
753 309 191 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1317
Size: 1054 263
MscI TGG'CCA
Cuts at: 0 346 1317
Size: 346 971
MslI CAynn'nnrTG
Cuts at: 0 340 1317
Size: 340 977
MspA1I CmG'CkG
Cuts at: 0 383 1098 1317
Size: 383 715 219
Fragments arranged by size:
715 383 219
NaeI GCC'GGC
Cuts at: 0 305 1317
Size: 305 1012
NcoI C'CATG_G
Cuts at: 0 341 1317
Size: 341 976
NgoMIV G'CCGG_C
Cuts at: 0 303 1317
Size: 303 1014
NsiI A_TGCA'T
Cuts at: 0 954 1317
Size: 954 363
NspI r_CATG'y
Cuts at: 0 561 1317
Size: 561 756
PciI A'CATG_T
Cuts at: 0 557 1317
Size: 557 760
PfoI T'CCnGG_A
Cuts at: 0 50 1317
Size: 50 1267
PpuMI rG'GwC_Cy
Cuts at: 0 1253 1317
Size: 1253 64
PspOMI G'GGCC_C
Cuts at: 0 701 1309 1317
Size: 701 608 8
Fragments arranged by size:
701 608 8
PstI C_TGCA'G
Cuts at: 0 167 886 1317
Size: 167 719 431
Fragments arranged by size:
719 431 167
SfcI C'TryA_G
Cuts at: 0 163 882 1317
Size: 163 719 435
Fragments arranged by size:
719 435 163
SmaI CCC'GGG
Cuts at: 0 620 728 753 1317
Size: 620 108 25 564
Fragments arranged by size:
620 564 108 25
StyI C'CwwG_G
Cuts at: 0 268 341 906 1139 1317
Size: 268 73 565 233 178
Fragments arranged by size:
565 268 233 178 73
XmaI C'CCGG_G
Cuts at: 0 618 726 751 1317
Size: 618 108 25 566
Fragments arranged by size:
618 566 108 25
XmnI GAAnn'nnTTC
Cuts at: 0 595 1317
Size: 595 722
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BplI
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstYI BtgI
BtgZI ClaI DrdI EaeI EagI EciI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PciI PfoI PpuMI
PspOMI PstI SfcI SmaI StyI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BglII BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI CspCI DraI
DraIII EarI EcoICRI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI ZraI