Restriction map for
pF1KB9598
(Linear) (Six-base) MAPSORT of: KIBB9598.seq Check: 8067 from: 1 to: 1434
KIBB9598.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 740 1434
Size: 740 694
AfeI AGC'GCT
Cuts at: 0 1280 1434
Size: 1280 154
AflIII A'CryG_T
Cuts at: 0 32 668 1434
Size: 32 636 766
Fragments arranged by size:
766 636 32
AgeI A'CCGG_T
Cuts at: 0 933 1434
Size: 933 501
AhdI GACnn_n'nnGTC
Cuts at: 0 657 1434
Size: 657 777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 706 1434
Size: 706 728
AlwNI CAG_nnn'CTG
Cuts at: 0 234 1368 1434
Size: 234 1134 66
Fragments arranged by size:
1134 234 66
ApaI G_GGCC'C
Cuts at: 0 1162 1434
Size: 1162 272
AscI GG'CGCG_CC
Cuts at: 0 290 1434
Size: 290 1144
AvaI C'yCGr_G
Cuts at: 0 393 624 966 1014 1258 1434
Size: 393 231 342 48 244 176
Fragments arranged by size:
393 342 244 231 176 48
BanI G'GyrC_C
Cuts at: 0 180 192 695 796 850 930 1045 1075
Size: 180 12 503 101 54 80 115 30
Cuts at: 1075 1084 1434
Size: 9 350
Fragments arranged by size:
503 350 180 115 101 80 54 30
12 9
BanII G_rGCy'C
Cuts at: 0 472 1162 1180 1434
Size: 472 690 18 254
Fragments arranged by size:
690 472 254 18
BbeI G_GCGC'C
Cuts at: 0 699 800 1049 1079 1434
Size: 699 101 249 30 355
Fragments arranged by size:
699 355 249 101 30
BbvCI CC'TCA_GC
Cuts at: 0 212 1434
Size: 212 1222
BglI GCCn_nnn'nGGC
Cuts at: 0 824 1054 1434
Size: 824 230 380
Fragments arranged by size:
824 380 230
BlpI GC'TnA_GC
Cuts at: 0 1309 1434
Size: 1309 125
Bme1580I G_kGCm'C
Cuts at: 0 185 1069 1087 1162 1434
Size: 185 884 18 75 272
Fragments arranged by size:
884 272 185 75 18
BmgBI CAC'GTC
Cuts at: 0 66 285 1434
Size: 66 219 1149
Fragments arranged by size:
1149 219 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 1220 1434
Size: 1220 214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1434
Size: 886 548
Bpu10I CC'TnA_GC
Cuts at: 0 212 490 1434
Size: 212 278 944
Fragments arranged by size:
944 278 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1434
Size: 1397 37
BsaHI Gr'CG_yC
Cuts at: 0 696 797 1046 1076 1434
Size: 696 101 249 30 358
Fragments arranged by size:
696 358 249 101 30
BsaWI w'CCGG_w
Cuts at: 0 933 1357 1434
Size: 933 424 77
Fragments arranged by size:
933 424 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1434
Size: 346 1088
BseYI C'CCAG_C
Cuts at: 0 975 1192 1434
Size: 975 217 242
Fragments arranged by size:
975 242 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1288 1434
Size: 814 474 146
Fragments arranged by size:
814 474 146
BsiEI CG_ry'CG
Cuts at: 0 59 257 794 816 848 881 992 1058
Size: 59 198 537 22 32 33 111 66
Cuts at: 1058 1126 1388 1406 1434
Size: 68 262 18 28
Fragments arranged by size:
537 262 198 111 68 66 59 33
32 28 22 18
BsiHKAI G_wGCw'C
Cuts at: 0 731 1434
Size: 731 703
BsiWI C'GTAC_G
Cuts at: 0 911 1434
Size: 911 523
Bsp1286I G_dGCh'C
Cuts at: 0 185 472 731 1069 1087 1162 1180 1434
Size: 185 287 259 338 18 75 18 254
Fragments arranged by size:
338 287 259 254 185 75 18 18
BspEI T'CCGG_A
Cuts at: 0 1357 1434
Size: 1357 77
BspHI T'CATG_A
Cuts at: 0 752 1434
Size: 752 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 115 740 1434
Size: 115 625 694
Fragments arranged by size:
694 625 115
BsrBI CCG'CTC
Cuts at: 0 347 494 1411 1434
Size: 347 147 917 23
Fragments arranged by size:
917 347 147 23
BsrDI GCAATG_nn'
Cuts at: 0 238 1434
Size: 238 1196
BsrFI r'CCGG_y
Cuts at: 0 793 847 924 933 1201 1425 1434
Size: 793 54 77 9 268 224 9
Fragments arranged by size:
793 268 224 77 54 9 9
BssHII G'CGCG_C
Cuts at: 0 290 1118 1434
Size: 290 828 316
Fragments arranged by size:
828 316 290
BstYI r'GATC_y
Cuts at: 0 602 1434
Size: 602 832
BtgI C'CryG_G
Cuts at: 0 140 452 800 881 1052 1079 1163 1434
Size: 140 312 348 81 171 27 84 271
Fragments arranged by size:
348 312 271 171 140 84 81 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 423 1314 1434
Size: 423 891 120
Fragments arranged by size:
891 423 120
ClaI AT'CG_AT
Cuts at: 0 74 1434
Size: 74 1360
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1269 1434
Size: 595 674 165
Fragments arranged by size:
674 595 165
EaeI y'GGCC_r
Cuts at: 0 39 56 254 455 791 813 845 878
Size: 39 17 198 201 336 22 32 33
Cuts at: 878 989 1055 1123 1252 1286 1385 1403 1423
Size: 111 66 68 129 34 99 18 20
Cuts at: 1423 1434
Size: 11
Fragments arranged by size:
336 201 198 129 111 99 68 66
39 34 33 32 22 20 18 17
11
EagI C'GGCC_G
Cuts at: 0 56 254 791 813 845 878 989 1055
Size: 56 198 537 22 32 33 111 66
Cuts at: 1055 1123 1385 1403 1434
Size: 68 262 18 31
Fragments arranged by size:
537 262 198 111 68 66 56 33
32 31 22 18
EarI CTCTTCn'nnn_
Cuts at: 0 101 1434
Size: 101 1333
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 316 556 1434
Size: 187 129 240 878
Fragments arranged by size:
878 240 187 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1434
Size: 886 548
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 531 1434
Size: 166 365 903
Fragments arranged by size:
903 365 166
EcoO109I rG'GnC_Cy
Cuts at: 0 805 1158 1159 1434
Size: 805 353 1 275
Fragments arranged by size:
805 353 275 1
HaeII r_GCGC'y
Cuts at: 0 26 699 717 773 800 901 1049 1079
Size: 26 673 18 56 27 101 148 30
Cuts at: 1079 1193 1282 1434
Size: 114 89 152
Fragments arranged by size:
673 152 148 114 101 89 56 30
27 26 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 643 675 1434
Size: 643 32 759
Fragments arranged by size:
759 643 32
KasI G'GCGC_C
Cuts at: 0 695 796 1045 1075 1434
Size: 695 101 249 30 359
Fragments arranged by size:
695 359 249 101 30
MscI TGG'CCA
Cuts at: 0 457 1288 1434
Size: 457 831 146
Fragments arranged by size:
831 457 146
MslI CAynn'nnrTG
Cuts at: 0 76 451 1434
Size: 76 375 983
Fragments arranged by size:
983 375 76
MspA1I CmG'CkG
Cuts at: 0 142 371 802 871 883 949 957 1026
Size: 142 229 431 69 12 66 8 69
Cuts at: 1026 1054 1060 1072 1081 1165 1302 1396 1434
Size: 28 6 12 9 84 137 94 38
Fragments arranged by size:
431 229 142 137 94 84 69 69
66 38 28 12 12 9 8 6
NaeI GCC'GGC
Cuts at: 0 795 1203 1427 1434
Size: 795 408 224 7
Fragments arranged by size:
795 408 224 7
NarI GG'CG_CC
Cuts at: 0 696 797 1046 1076 1434
Size: 696 101 249 30 358
Fragments arranged by size:
696 358 249 101 30
NcoI C'CATG_G
Cuts at: 0 452 1434
Size: 452 982
NgoMIV G'CCGG_C
Cuts at: 0 793 1201 1425 1434
Size: 793 408 224 9
Fragments arranged by size:
793 408 224 9
NotI GC'GGCC_GC
Cuts at: 0 878 989 1055 1403 1434
Size: 878 111 66 348 31
Fragments arranged by size:
878 348 111 66 31
NspI r_CATG'y
Cuts at: 0 36 672 1434
Size: 36 636 762
Fragments arranged by size:
762 636 36
PciI A'CATG_T
Cuts at: 0 32 668 1434
Size: 32 636 766
Fragments arranged by size:
766 636 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 1434
Size: 655 32 747
Fragments arranged by size:
747 655 32
PpuMI rG'GwC_Cy
Cuts at: 0 805 1434
Size: 805 629
PspOMI G'GGCC_C
Cuts at: 0 1158 1434
Size: 1158 276
PstI C_TGCA'G
Cuts at: 0 467 1025 1031 1373 1434
Size: 467 558 6 342 61
Fragments arranged by size:
558 467 342 61 6
PvuII CAG'CTG
Cuts at: 0 1026 1434
Size: 1026 408
RsrII CG'GwC_CG
Cuts at: 0 271 1434
Size: 271 1163
SacII CC_GC'GG
Cuts at: 0 143 803 884 1055 1082 1166 1434
Size: 143 660 81 171 27 84 268
Fragments arranged by size:
660 268 171 143 84 81 27
SanDI GG'GwC_CC
Cuts at: 0 805 1434
Size: 805 629
SfcI C'TryA_G
Cuts at: 0 463 531 1021 1027 1369 1434
Size: 463 68 490 6 342 65
Fragments arranged by size:
490 463 342 68 65 6
SfoI GGC'GCC
Cuts at: 0 697 798 1047 1077 1434
Size: 697 101 249 30 357
Fragments arranged by size:
697 357 249 101 30
SgrAI Cr'CCGG_yG
Cuts at: 0 924 933 1201 1434
Size: 924 9 268 233
Fragments arranged by size:
924 268 233 9
SmaI CCC'GGG
Cuts at: 0 626 1016 1260 1434
Size: 626 390 244 174
Fragments arranged by size:
626 390 244 174
SmlI C'TyrA_G
Cuts at: 0 1412 1434
Size: 1412 22
SrfI GCCC'GGGC
Cuts at: 0 1016 1434
Size: 1016 418
StuI AGG'CCT
Cuts at: 0 386 1434
Size: 386 1048
StyI C'CwwG_G
Cuts at: 0 245 452 1434
Size: 245 207 982
Fragments arranged by size:
982 245 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1306 1434
Size: 1306 128
XmaI C'CCGG_G
Cuts at: 0 624 1014 1258 1434
Size: 624 390 244 176
Fragments arranged by size:
624 390 244 176
XmnI GAAnn'nnTTC
Cuts at: 0 706 1434
Size: 706 728
Enzymes that do cut:
AarI AfeI AflIII AgeI AhdI AlfI AlwNI ApaI
AscI AvaI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BstYI BtgI
BtgZI ClaI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PpiI
PpuMI PspOMI PstI PvuII RsrII SacII SanDI SfcI
SfoI SgrAI SmaI SmlI SrfI StuI StyI TaqII
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AleI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglII BmtI BplI BsaI
BsaAI BsaBI BsaXI BsmI BsmBI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI CspCI DraI
DraIII EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI