Restriction map for
pF1KB9594
(Linear) (Six-base) MAPSORT of: KIBB9594.seq Check: 5297 from: 1 to: 975
KIBB9594.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 446 975
Size: 446 529
AfeI AGC'GCT
Cuts at: 0 166 322 975
Size: 166 156 653
Fragments arranged by size:
653 166 156
AflII C'TTAA_G
Cuts at: 0 334 975
Size: 334 641
AflIII A'CryG_T
Cuts at: 0 20 284 648 650 975
Size: 20 264 364 2 325
Fragments arranged by size:
364 325 264 20 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 454 975
Size: 322 132 521
Fragments arranged by size:
521 322 132
AlwNI CAG_nnn'CTG
Cuts at: 0 257 384 975
Size: 257 127 591
Fragments arranged by size:
591 257 127
ApaLI G'TGCA_C
Cuts at: 0 970 975
Size: 970 5
AvaI C'yCGr_G
Cuts at: 0 88 426 489 975
Size: 88 338 63 486
Fragments arranged by size:
486 338 88 63
BanI G'GyrC_C
Cuts at: 0 311 351 579 634 975
Size: 311 40 228 55 341
Fragments arranged by size:
341 311 228 55 40
BanII G_rGCy'C
Cuts at: 0 85 975
Size: 85 890
BbeI G_GCGC'C
Cuts at: 0 315 355 975
Size: 315 40 620
Fragments arranged by size:
620 315 40
BglI GCCn_nnn'nGGC
Cuts at: 0 373 552 975
Size: 373 179 423
Fragments arranged by size:
423 373 179
BglII A'GATC_T
Cuts at: 0 113 975
Size: 113 862
BlpI GC'TnA_GC
Cuts at: 0 106 409 871 975
Size: 106 303 462 104
Fragments arranged by size:
462 303 106 104
Bme1580I G_kGCm'C
Cuts at: 0 433 584 637 785 812 974 975
Size: 433 151 53 148 27 162 1
Fragments arranged by size:
433 162 151 148 53 27 1
BmgBI CAC'GTC
Cuts at: 0 881 975
Size: 881 94
BsaAI yAC'GTr
Cuts at: 0 23 651 975
Size: 23 628 324
Fragments arranged by size:
628 324 23
BsaHI Gr'CG_yC
Cuts at: 0 312 352 975
Size: 312 40 623
Fragments arranged by size:
623 312 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 975
Size: 178 30 767
Fragments arranged by size:
767 178 30
BseYI C'CCAG_C
Cuts at: 0 355 630 922 975
Size: 355 275 292 53
Fragments arranged by size:
355 292 275 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 975
Size: 250 725
BsiEI CG_ry'CG
Cuts at: 0 15 232 803 975
Size: 15 217 571 172
Fragments arranged by size:
571 217 172 15
BsiHKAI G_wGCw'C
Cuts at: 0 85 974 975
Size: 85 889 1
Fragments arranged by size:
889 85 1
Bsp1286I G_dGCh'C
Cuts at: 0 85 433 584 637 785 812 974 975
Size: 85 348 151 53 148 27 162 1
Fragments arranged by size:
348 162 151 148 85 53 27 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 446 975
Size: 392 54 529
Fragments arranged by size:
529 392 54
BsrBI CCG'CTC
Cuts at: 0 597 975
Size: 597 378
BsrFI r'CCGG_y
Cuts at: 0 576 637 664 798 975
Size: 576 61 27 134 177
Fragments arranged by size:
576 177 134 61 27
BssHII G'CGCG_C
Cuts at: 0 413 975
Size: 413 562
BstAPI GCAn_nnn'nTGC
Cuts at: 0 384 579 975
Size: 384 195 396
Fragments arranged by size:
396 384 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 696 975
Size: 696 279
BstYI r'GATC_y
Cuts at: 0 113 218 975
Size: 113 105 757
Fragments arranged by size:
757 113 105
BtgI C'CryG_G
Cuts at: 0 223 617 695 975
Size: 223 394 78 280
Fragments arranged by size:
394 280 223 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 510 975
Size: 510 465
DraIII CAC_nnn'GTG
Cuts at: 0 963 975
Size: 963 12
EaeI y'GGCC_r
Cuts at: 0 12 71 229 643 680 800 975
Size: 12 59 158 414 37 120 175
Fragments arranged by size:
414 175 158 120 59 37 12
EagI C'GGCC_G
Cuts at: 0 12 229 800 975
Size: 12 217 571 175
Fragments arranged by size:
571 217 175 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 555 651 939 975
Size: 555 96 288 36
Fragments arranged by size:
555 288 96 36
EcoICRI GAG'CTC
Cuts at: 0 83 975
Size: 83 892
EcoNI CCTnn'n_nnAGG
Cuts at: 0 147 975
Size: 147 828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 522 975
Size: 270 252 453
Fragments arranged by size:
453 270 252
HaeII r_GCGC'y
Cuts at: 0 168 265 315 324 355 719 835 975
Size: 168 97 50 9 31 364 116 140
Fragments arranged by size:
364 168 140 116 97 50 31 9
HincII GTy'rAC
Cuts at: 0 611 975
Size: 611 364
KasI G'GCGC_C
Cuts at: 0 311 351 975
Size: 311 40 624
Fragments arranged by size:
624 311 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 975
Size: 365 610
MscI TGG'CCA
Cuts at: 0 73 645 975
Size: 73 572 330
Fragments arranged by size:
572 330 73
MslI CAynn'nnrTG
Cuts at: 0 653 694 778 975
Size: 653 41 84 197
Fragments arranged by size:
653 197 84 41
MspA1I CmG'CkG
Cuts at: 0 105 225 586 741 975
Size: 105 120 361 155 234
Fragments arranged by size:
361 234 155 120 105
NaeI GCC'GGC
Cuts at: 0 666 800 975
Size: 666 134 175
Fragments arranged by size:
666 175 134
NarI GG'CG_CC
Cuts at: 0 312 352 975
Size: 312 40 623
Fragments arranged by size:
623 312 40
NcoI C'CATG_G
Cuts at: 0 617 695 975
Size: 617 78 280
Fragments arranged by size:
617 280 78
NgoMIV G'CCGG_C
Cuts at: 0 664 798 975
Size: 664 134 177
Fragments arranged by size:
664 177 134
NspI r_CATG'y
Cuts at: 0 288 975
Size: 288 687
PciI A'CATG_T
Cuts at: 0 284 975
Size: 284 691
PmlI CAC'GTG
Cuts at: 0 651 975
Size: 651 324
PstI C_TGCA'G
Cuts at: 0 389 975
Size: 389 586
SacI G_AGCT'C
Cuts at: 0 85 975
Size: 85 890
SacII CC_GC'GG
Cuts at: 0 226 975
Size: 226 749
SfcI C'TryA_G
Cuts at: 0 147 385 711 975
Size: 147 238 326 264
Fragments arranged by size:
326 264 238 147
SfoI GGC'GCC
Cuts at: 0 313 353 975
Size: 313 40 622
Fragments arranged by size:
622 313 40
SmlI C'TyrA_G
Cuts at: 0 334 975
Size: 334 641
StyI C'CwwG_G
Cuts at: 0 617 695 975
Size: 617 78 280
Fragments arranged by size:
617 280 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 284 975
Size: 284 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 975
Size: 178 32 765
Fragments arranged by size:
765 178 32
Enzymes that do cut:
AarI AfeI AflII AflIII AlfI AlwNI ApaLI AvaI
BanI BanII BbeI BglI BglII BlpI Bme1580I BmgBI
BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EciI EcoICRI EcoNI FalI
HaeII HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmlI PstI SacI
SacII SfcI SfoI SmlI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BseRI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EarI Eco57MI EcoO109I EcoRI EcoRV FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI