Restriction map for
pF1KB9593
(Linear) (Six-base) MAPSORT of: KIBB9593.seq Check: 159 from: 1 to: 990
KIBB9593.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 892 990
Size: 892 98
AflIII A'CryG_T
Cuts at: 0 299 990
Size: 299 691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 990
Size: 371 619
AlwNI CAG_nnn'CTG
Cuts at: 0 15 272 358 679 762 990
Size: 15 257 86 321 83 228
Fragments arranged by size:
321 257 228 86 83 15
ApaI G_GGCC'C
Cuts at: 0 376 861 990
Size: 376 485 129
Fragments arranged by size:
485 376 129
ApoI r'AATT_y
Cuts at: 0 765 990
Size: 765 225
AvaI C'yCGr_G
Cuts at: 0 19 368 990
Size: 19 349 622
Fragments arranged by size:
622 349 19
BanI G'GyrC_C
Cuts at: 0 28 234 364 990
Size: 28 206 130 626
Fragments arranged by size:
626 206 130 28
BanII G_rGCy'C
Cuts at: 0 100 376 575 617 861 990
Size: 100 276 199 42 244 129
Fragments arranged by size:
276 244 199 129 100 42
BbeI G_GCGC'C
Cuts at: 0 32 990
Size: 32 958
BbvCI CC'TCA_GC
Cuts at: 0 359 810 990
Size: 359 451 180
Fragments arranged by size:
451 359 180
BglI GCCn_nnn'nGGC
Cuts at: 0 183 990
Size: 183 807
Bme1580I G_kGCm'C
Cuts at: 0 239 367 376 535 861 990
Size: 239 128 9 159 326 129
Fragments arranged by size:
326 239 159 129 128 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 990
Size: 827 163
Bpu10I CC'TnA_GC
Cuts at: 0 359 490 810 990
Size: 359 131 320 180
Fragments arranged by size:
359 320 180 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 990
Size: 814 176
BsaHI Gr'CG_yC
Cuts at: 0 29 967 990
Size: 29 938 23
Fragments arranged by size:
938 29 23
BsaWI w'CCGG_w
Cuts at: 0 962 990
Size: 962 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 39 69 990
Size: 39 30 921
Fragments arranged by size:
921 39 30
BseYI C'CCAG_C
Cuts at: 0 534 645 990
Size: 534 111 345
Fragments arranged by size:
534 345 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 990
Size: 812 178
BsiEI CG_ry'CG
Cuts at: 0 443 990
Size: 443 547
BsiHKAI G_wGCw'C
Cuts at: 0 100 311 457 617 800 990
Size: 100 211 146 160 183 190
Fragments arranged by size:
211 190 183 160 146 100
Bsp1286I G_dGCh'C
Cuts at: 0 100 239 311 367 376 457 535 575
Size: 100 139 72 56 9 81 78 40
Cuts at: 575 617 800 861 990
Size: 42 183 61 129
Fragments arranged by size:
183 139 129 100 81 78 72 61
56 42 40 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 892 990
Size: 892 98
BsrFI r'CCGG_y
Cuts at: 0 173 442 664 990
Size: 173 269 222 326
Fragments arranged by size:
326 269 222 173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 452 990
Size: 15 437 538
Fragments arranged by size:
538 437 15
BstYI r'GATC_y
Cuts at: 0 477 990
Size: 477 513
Bsu36I CC'TnA_GG
Cuts at: 0 400 990
Size: 400 590
BtgI C'CryG_G
Cuts at: 0 390 566 620 990
Size: 390 176 54 370
Fragments arranged by size:
390 370 176 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 54 257 990
Size: 54 203 733
Fragments arranged by size:
733 203 54
BtsI GCAGTG_nn'
Cuts at: 0 457 862 990
Size: 457 405 128
Fragments arranged by size:
457 405 128
DraIII CAC_nnn'GTG
Cuts at: 0 391 990
Size: 391 599
DrdI GACnn_nn'nnGTC
Cuts at: 0 424 990
Size: 424 566
EaeI y'GGCC_r
Cuts at: 0 86 559 623 990
Size: 86 473 64 367
Fragments arranged by size:
473 367 86 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 990
Size: 187 803
EcoICRI GAG'CTC
Cuts at: 0 98 615 990
Size: 98 517 375
Fragments arranged by size:
517 375 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 990
Size: 827 163
EcoO109I rG'GnC_Cy
Cuts at: 0 372 373 417 486 696 858 990
Size: 372 1 44 69 210 162 132
Fragments arranged by size:
372 210 162 132 69 44 1
HaeII r_GCGC'y
Cuts at: 0 32 990
Size: 32 958
HindIII A'AGCT_T
Cuts at: 0 492 990
Size: 492 498
KasI G'GCGC_C
Cuts at: 0 28 990
Size: 28 962
MscI TGG'CCA
Cuts at: 0 88 625 990
Size: 88 537 365
Fragments arranged by size:
537 365 88
MspA1I CmG'CkG
Cuts at: 0 111 990
Size: 111 879
NaeI GCC'GGC
Cuts at: 0 666 990
Size: 666 324
NarI GG'CG_CC
Cuts at: 0 29 990
Size: 29 961
NcoI C'CATG_G
Cuts at: 0 566 990
Size: 566 424
NgoMIV G'CCGG_C
Cuts at: 0 664 990
Size: 664 326
NspI r_CATG'y
Cuts at: 0 303 605 990
Size: 303 302 385
Fragments arranged by size:
385 303 302
PasI CC'CwG_GG
Cuts at: 0 607 893 990
Size: 607 286 97
Fragments arranged by size:
607 286 97
PciI A'CATG_T
Cuts at: 0 299 990
Size: 299 691
PflMI CCAn_nnn'nTGG
Cuts at: 0 652 990
Size: 652 338
PpuMI rG'GwC_Cy
Cuts at: 0 417 990
Size: 417 573
PspOMI G'GGCC_C
Cuts at: 0 372 857 990
Size: 372 485 133
Fragments arranged by size:
485 372 133
PstI C_TGCA'G
Cuts at: 0 758 990
Size: 758 232
SacI G_AGCT'C
Cuts at: 0 100 617 990
Size: 100 517 373
Fragments arranged by size:
517 373 100
SfcI C'TryA_G
Cuts at: 0 60 754 990
Size: 60 694 236
Fragments arranged by size:
694 236 60
SfoI GGC'GCC
Cuts at: 0 30 990
Size: 30 960
SmaI CCC'GGG
Cuts at: 0 21 370 990
Size: 21 349 620
Fragments arranged by size:
620 349 21
SmlI C'TyrA_G
Cuts at: 0 793 990
Size: 793 197
SphI G_CATG'C
Cuts at: 0 605 990
Size: 605 385
SrfI GCCC'GGGC
Cuts at: 0 21 990
Size: 21 969
StuI AGG'CCT
Cuts at: 0 555 791 990
Size: 555 236 199
Fragments arranged by size:
555 236 199
StyI C'CwwG_G
Cuts at: 0 380 482 566 990
Size: 380 102 84 424
Fragments arranged by size:
424 380 102 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 849 990
Size: 579 270 141
Fragments arranged by size:
579 270 141
XmaI C'CCGG_G
Cuts at: 0 19 368 990
Size: 19 349 622
Fragments arranged by size:
622 349 19
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BglI Bme1580I BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII
HindIII KasI MscI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PpuMI PspOMI PstI SacI
SfcI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI
BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI EagI EarI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HpaI KpnI MfeI
MluI MmeI MslI NdeI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI