Restriction map for
pF1KB9590
(Linear) (Six-base) MAPSORT of: KIBB9590.seq Check: 5516 from: 1 to: 1215
KIBB9590.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1215
Size: 44 1171
AleI CACnn'nnGTG
Cuts at: 0 877 1215
Size: 877 338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 1215
Size: 250 32 933
Fragments arranged by size:
933 250 32
ApoI r'AATT_y
Cuts at: 0 954 1215
Size: 954 261
AvaI C'yCGr_G
Cuts at: 0 109 1215
Size: 109 1106
BbsI GAAGACnn'nnnn_
Cuts at: 0 796 941 1215
Size: 796 145 274
Fragments arranged by size:
796 274 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1074 1108 1215
Size: 1074 34 107
Fragments arranged by size:
1074 107 34
Bme1580I G_kGCm'C
Cuts at: 0 764 1215
Size: 764 451
BmgBI CAC'GTC
Cuts at: 0 468 1215
Size: 468 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 994 1116 1215
Size: 516 478 122 99
Fragments arranged by size:
516 478 122 99
Bpu10I CC'TnA_GC
Cuts at: 0 998 1215
Size: 998 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1215
Size: 25 1190
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 1135 1215
Size: 183 952 80
Fragments arranged by size:
952 183 80
BsaAI yAC'GTr
Cuts at: 0 759 984 1052 1215
Size: 759 225 68 163
Fragments arranged by size:
759 225 163 68
BsaWI w'CCGG_w
Cuts at: 0 1108 1215
Size: 1108 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1114 1144 1215
Size: 1114 30 71
Fragments arranged by size:
1114 71 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 264 730 1171 1180 1183 1215
Size: 264 466 441 9 3 32
Fragments arranged by size:
466 441 264 32 9 3
BseYI C'CCAG_C
Cuts at: 0 235 1146 1215
Size: 235 911 69
Fragments arranged by size:
911 235 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1215
Size: 385 830
BsiEI CG_ry'CG
Cuts at: 0 736 1215
Size: 736 479
BsiWI C'GTAC_G
Cuts at: 0 980 1215
Size: 980 235
BsmI GAATG_Cn'
Cuts at: 0 264 1215
Size: 264 951
BsmBI CGTCTCn'nnnn_
Cuts at: 0 352 1215
Size: 352 863
Bsp1286I G_dGCh'C
Cuts at: 0 764 1215
Size: 764 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 711 1021 1215
Size: 711 310 194
Fragments arranged by size:
711 310 194
BsrBI CCG'CTC
Cuts at: 0 108 1215
Size: 108 1107
BsrDI GCAATG_nn'
Cuts at: 0 1079 1215
Size: 1079 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 123 1215
Size: 123 1092
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 1215
Size: 957 258
BstYI r'GATC_y
Cuts at: 0 1114 1215
Size: 1114 101
BtgI C'CryG_G
Cuts at: 0 77 1215
Size: 77 1138
EaeI y'GGCC_r
Cuts at: 0 80 515 733 947 1097 1215
Size: 80 435 218 214 150 118
Fragments arranged by size:
435 218 214 150 118 80
EagI C'GGCC_G
Cuts at: 0 733 1215
Size: 733 482
EarI CTCTTCn'nnn_
Cuts at: 0 610 1157 1215
Size: 610 547 58
Fragments arranged by size:
610 547 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 516 994 1116 1215
Size: 44 472 478 122 99
Fragments arranged by size:
478 472 122 99 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 215 1215
Size: 215 1000
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 138 1215
Size: 138 1077
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 1215
Size: 808 32 375
Fragments arranged by size:
808 375 32
HincII GTy'rAC
Cuts at: 0 153 1215
Size: 153 1062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1215
Size: 839 376
MscI TGG'CCA
Cuts at: 0 517 949 1099 1215
Size: 517 432 150 116
Fragments arranged by size:
517 432 150 116
MslI CAynn'nnrTG
Cuts at: 0 211 757 877 1215
Size: 211 546 120 338
Fragments arranged by size:
546 338 211 120
MspA1I CmG'CkG
Cuts at: 0 732 738 1215
Size: 732 6 477
Fragments arranged by size:
732 477 6
NotI GC'GGCC_GC
Cuts at: 0 733 1215
Size: 733 482
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 396 711 1215
Size: 168 228 315 504
Fragments arranged by size:
504 315 228 168
PfoI T'CCnGG_A
Cuts at: 0 916 1215
Size: 916 299
PmlI CAC'GTG
Cuts at: 0 759 1052 1215
Size: 759 293 163
Fragments arranged by size:
759 293 163
PstI C_TGCA'G
Cuts at: 0 808 1215
Size: 808 407
SexAI A'CCwGG_T
Cuts at: 0 689 1215
Size: 689 526
SfcI C'TryA_G
Cuts at: 0 667 804 1215
Size: 667 137 411
Fragments arranged by size:
667 411 137
SmlI C'TyrA_G
Cuts at: 0 40 1215
Size: 40 1175
StyI C'CwwG_G
Cuts at: 0 283 1215
Size: 283 932
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 1215
Size: 249 966
TatI w'GTAC_w
Cuts at: 0 1121 1215
Size: 1121 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 1215
Size: 75 1140
Enzymes that do cut:
AcuI AleI AloI ApoI AvaI BbsI BcgI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BstAPI BstXI BstYI BtgI EaeI
EagI EarI Eco57MI EcoNI FalI Hin4I HincII MmeI
MscI MslI MspA1I NotI PflMI PfoI PmlI PstI
SexAI SfcI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI BsaBI BsaHI BsiHKAI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI