(Linear) (Six-base) MAPSORT of: KIBB9588.seq  Check: 6118  from: 1  to: 699



KIBB9588.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AflII C'TTAA_G

Cuts at:      0     334     699 
   Size:        334     365
 
AflIII A'CryG_T

Cuts at:      0     351     699 
   Size:        351     348
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      38     699 
   Size:         38     661
 
ApaLI G'TGCA_C

Cuts at:      0      23     699 
   Size:         23     676
 
ApoI r'AATT_y

Cuts at:      0     618     699 
   Size:        618      81
 
AseI AT'TA_AT

Cuts at:      0     260     699 
   Size:        260     439
 
BanII G_rGCy'C

Cuts at:      0     298     699 
   Size:        298     401
 
BclI T'GATC_A

Cuts at:      0     216     699 
   Size:        216     483
 
BglII A'GATC_T

Cuts at:      0     330     699 
   Size:        330     369
 
BlpI GC'TnA_GC

Cuts at:      0      87     699 
   Size:         87     612
 
Bme1580I G_kGCm'C

Cuts at:      0      27     699 
   Size:         27     672
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     699 
   Size:        423     276
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     391     699 
   Size:        391     308
 
BsaAI yAC'GTr

Cuts at:      0     354     699 
   Size:        354     345
 
BsiHKAI G_wGCw'C

Cuts at:      0      27     699 
   Size:         27     672
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     298     699 
   Size:         27     271     401

  Fragments arranged by size: 

                 401     271      27
 
BspHI T'CATG_A

Cuts at:      0     410     699 
   Size:        410     289
 
BsrGI T'GTAC_A

Cuts at:      0     348     699 
   Size:        348     351
 
BssSI C'ACGA_G

Cuts at:      0     220     699 
   Size:        220     479
 
BstYI r'GATC_y

Cuts at:      0     330     699 
   Size:        330     369
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     699 
   Size:        311     388
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     157     699 
   Size:        157     542
 
HincII GTy'rAC

Cuts at:      0     433     699 
   Size:        433     266
 
MfeI C'AATT_G

Cuts at:      0     585     699 
   Size:        585     114
 
MspA1I CmG'CkG

Cuts at:      0     448     699 
   Size:        448     251
 
PasI CC'CwG_GG

Cuts at:      0     298     699 
   Size:        298     401
 
PmlI CAC'GTG

Cuts at:      0     354     699 
   Size:        354     345
 
PsiI TTA'TAA

Cuts at:      0     367     699 
   Size:        367     332
 
PvuII CAG'CTG

Cuts at:      0     448     699 
   Size:        448     251
 
SmlI C'TyrA_G

Cuts at:      0     334     402     699 
   Size:        334      68     297

  Fragments arranged by size: 

                 334     297      68
 
SspI AAT'ATT

Cuts at:      0      75     699 
   Size:         75     624
 
TatI w'GTAC_w

Cuts at:      0     348     589     699 
   Size:        348     241     110

  Fragments arranged by size: 

                 348     241     110
 
XmnI GAAnn'nnTTC

Cuts at:      0      74     197     488     699 
   Size:         74     123     291     211

  Fragments arranged by size: 

                 291     211     123      74
 

 Enzymes that do cut:

    AflII   AflIII     AlfI    ApaLI     ApoI     AseI    BanII     BclI
    BglII     BlpI Bme1580I    BpuEI     BsaI    BsaAI  BsiHKAI Bsp1286I
    BspHI    BsrGI    BssSI    BstYI     EarI     EciI   HincII     MfeI
   MspA1I     PasI     PmlI     PsiI    PvuII     SmlI     SspI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI