(Linear) (Six-base) MAPSORT of: KIBB9586.seq  Check: 5767  from: 1  to: 450



KIBB9586.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

BanI G'GyrC_C

Cuts at:      0     102     450 
   Size:        102     348
 
BbvCI CC'TCA_GC

Cuts at:      0      95     450 
   Size:         95     355
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     450 
   Size:         14     436
 
Bpu10I CC'TnA_GC

Cuts at:      0      95     450 
   Size:         95     355
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388     450 
   Size:        388      62
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     450 
   Size:        137     313
 
BseYI C'CCAG_C

Cuts at:      0     142     450 
   Size:        142     308
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78     450 
   Size:         78     372
 
BsrFI r'CCGG_y

Cuts at:      0     236     450 
   Size:        236     214
 
BtgI C'CryG_G

Cuts at:      0     170     450 
   Size:        170     280
 
BtsI GCAGTG_nn'

Cuts at:      0     268     450 
   Size:        268     182
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     450 
   Size:        290     160
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     450 
   Size:         14     436
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     119     450 
   Size:        119     331
 
EcoRV GAT'ATC

Cuts at:      0      11     450 
   Size:         11     439
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299     450 
   Size:        267      32     151

  Fragments arranged by size: 

                 267     151      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     450 
   Size:        290     160
 
MslI CAynn'nnrTG

Cuts at:      0     223     450 
   Size:        223     227
 
MspA1I CmG'CkG

Cuts at:      0      77      99     450 
   Size:         77      22     351

  Fragments arranged by size: 

                 351      77      22
 
NcoI C'CATG_G

Cuts at:      0     170     450 
   Size:        170     280
 
NspI r_CATG'y

Cuts at:      0      73     376     450 
   Size:         73     303      74

  Fragments arranged by size: 

                 303      74      73
 
PasI CC'CwG_GG

Cuts at:      0     115     450 
   Size:        115     335
 
PstI C_TGCA'G

Cuts at:      0     275     450 
   Size:        275     175
 
PvuII CAG'CTG

Cuts at:      0      99     450 
   Size:         99     351
 
SfcI C'TryA_G

Cuts at:      0     271     450 
   Size:        271     179
 
SmlI C'TyrA_G

Cuts at:      0     367     450 
   Size:        367      83
 
SphI G_CATG'C

Cuts at:      0     376     450 
   Size:        376      74
 
StyI C'CwwG_G

Cuts at:      0     170     335     450 
   Size:        170     165     115

  Fragments arranged by size: 

                 170     165     115
 
TatI w'GTAC_w

Cuts at:      0     348     450 
   Size:        348     102
 

 Enzymes that do cut:

     BanI    BbvCI     BpmI   Bpu10I    BpuEI    BseRI    BseYI    BspMI
    BsrFI     BtgI     BtsI     EarI  Eco57MI    EcoNI    EcoRV    Hin4I
     MmeI     MslI   MspA1I     NcoI     NspI     PasI     PstI    PvuII
     SfcI     SmlI     SphI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI