Restriction map for
pF1KB9585
(Linear) (Six-base) MAPSORT of: KIBB9585.seq Check: 6670 from: 1 to: 1035
KIBB9585.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1035
Size: 207 828
AscI GG'CGCG_CC
Cuts at: 0 248 1035
Size: 248 787
AvaI C'yCGr_G
Cuts at: 0 160 1003 1035
Size: 160 843 32
Fragments arranged by size:
843 160 32
BanI G'GyrC_C
Cuts at: 0 141 264 428 573 645 726 762 1035
Size: 141 123 164 145 72 81 36 273
Fragments arranged by size:
273 164 145 141 123 81 72 36
BanII G_rGCy'C
Cuts at: 0 518 542 712 917 971 1010 1035
Size: 518 24 170 205 54 39 25
Fragments arranged by size:
518 205 170 54 39 25 24
BbeI G_GCGC'C
Cuts at: 0 145 268 432 577 730 766 1035
Size: 145 123 164 145 153 36 269
Fragments arranged by size:
269 164 153 145 145 123 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 799 1035
Size: 799 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 862 1035
Size: 828 34 173
Fragments arranged by size:
828 173 34
BglI GCCn_nnn'nGGC
Cuts at: 0 139 698 760 1035
Size: 139 559 62 275
Fragments arranged by size:
559 275 139 62
BlpI GC'TnA_GC
Cuts at: 0 970 1035
Size: 970 65
BmgBI CAC'GTC
Cuts at: 0 677 1035
Size: 677 358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 910 931 1035
Size: 256 654 21 104
Fragments arranged by size:
654 256 104 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1035
Size: 502 533
BsaAI yAC'GTr
Cuts at: 0 417 1035
Size: 417 618
BsaHI Gr'CG_yC
Cuts at: 0 142 265 429 574 719 727 763 1035
Size: 142 123 164 145 145 8 36 272
Fragments arranged by size:
272 164 145 145 142 123 36 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 349 367 379 943 973 1035
Size: 337 12 18 12 564 30 62
Fragments arranged by size:
564 337 62 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 318 577 672 1011 1035
Size: 318 259 95 339 24
Fragments arranged by size:
339 318 259 95 24
BseYI C'CCAG_C
Cuts at: 0 24 186 243 1035
Size: 24 162 57 792
Fragments arranged by size:
792 162 57 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 463 1035
Size: 439 24 572
Fragments arranged by size:
572 439 24
BsiEI CG_ry'CG
Cuts at: 0 404 737 923 1035
Size: 404 333 186 112
Fragments arranged by size:
404 333 186 112
BsiHKAI G_wGCw'C
Cuts at: 0 518 917 971 1035
Size: 518 399 54 64
Fragments arranged by size:
518 399 64 54
BsiWI C'GTAC_G
Cuts at: 0 116 611 1035
Size: 116 495 424
Fragments arranged by size:
495 424 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 886 1035
Size: 886 149
Bsp1286I G_dGCh'C
Cuts at: 0 518 542 712 917 971 1010 1035
Size: 518 24 170 205 54 39 25
Fragments arranged by size:
518 205 170 54 39 25 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 762 1035
Size: 762 273
BsrBI CCG'CTC
Cuts at: 0 653 933 1035
Size: 653 280 102
Fragments arranged by size:
653 280 102
BsrFI r'CCGG_y
Cuts at: 0 178 399 759 1035
Size: 178 221 360 276
Fragments arranged by size:
360 276 221 178
BssHII G'CGCG_C
Cuts at: 0 210 248 449 621 1035
Size: 210 38 201 172 414
Fragments arranged by size:
414 210 201 172 38
BssSI C'ACGA_G
Cuts at: 0 205 1035
Size: 205 830
BstAPI GCAn_nnn'nTGC
Cuts at: 0 389 1035
Size: 389 646
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 1035
Size: 75 960
BstYI r'GATC_y
Cuts at: 0 824 1035
Size: 824 211
BtgI C'CryG_G
Cuts at: 0 68 137 149 272 278 542 797 1035
Size: 68 69 12 123 6 264 255 238
Fragments arranged by size:
264 255 238 123 69 68 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 1035
Size: 831 204
BtsI GCAGTG_nn'
Cuts at: 0 1029 1035
Size: 1029 6
DraI TTT'AAA
Cuts at: 0 63 1035
Size: 63 972
EaeI y'GGCC_r
Cuts at: 0 77 152 275 401 920 1026 1035
Size: 77 75 123 126 519 106 9
Fragments arranged by size:
519 126 123 106 77 75 9
EagI C'GGCC_G
Cuts at: 0 401 920 1035
Size: 401 519 115
Fragments arranged by size:
519 401 115
EarI CTCTTCn'nnn_
Cuts at: 0 367 1035
Size: 367 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 290 538 1035
Size: 112 178 248 497
Fragments arranged by size:
497 248 178 112
EcoICRI GAG'CTC
Cuts at: 0 516 915 969 1035
Size: 516 399 54 66
Fragments arranged by size:
516 399 66 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 256 910 931 1035
Size: 207 49 654 21 104
Fragments arranged by size:
654 207 104 49 21
EcoO109I rG'GnC_Cy
Cuts at: 0 21 258 906 1035
Size: 21 237 648 129
Fragments arranged by size:
648 237 129 21
FspI TGC'GCA
Cuts at: 0 856 883 1035
Size: 856 27 152
Fragments arranged by size:
856 152 27
HaeII r_GCGC'y
Cuts at: 0 145 268 432 577 730 766 1035
Size: 145 123 164 145 153 36 269
Fragments arranged by size:
269 164 153 145 145 123 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 349 369 381 943 975 1035
Size: 337 12 20 12 562 32 60
Fragments arranged by size:
562 337 60 32 20 12 12
KasI G'GCGC_C
Cuts at: 0 141 264 428 573 726 762 1035
Size: 141 123 164 145 153 36 273
Fragments arranged by size:
273 164 153 145 141 123 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 392 1035
Size: 380 12 643
Fragments arranged by size:
643 380 12
MscI TGG'CCA
Cuts at: 0 79 1035
Size: 79 956
MslI CAynn'nnrTG
Cuts at: 0 73 850 1035
Size: 73 777 185
Fragments arranged by size:
777 185 73
MspA1I CmG'CkG
Cuts at: 0 139 151 243 544 662 715 957 999
Size: 139 12 92 301 118 53 242 42
Cuts at: 999 1035
Size: 36
Fragments arranged by size:
301 242 139 118 92 53 42 36
12
NaeI GCC'GGC
Cuts at: 0 180 401 761 1035
Size: 180 221 360 274
Fragments arranged by size:
360 274 221 180
NarI GG'CG_CC
Cuts at: 0 142 265 429 574 727 763 1035
Size: 142 123 164 145 153 36 272
Fragments arranged by size:
272 164 153 145 142 123 36
NcoI C'CATG_G
Cuts at: 0 68 1035
Size: 68 967
NgoMIV G'CCGG_C
Cuts at: 0 178 399 759 1035
Size: 178 221 360 276
Fragments arranged by size:
360 276 221 178
NspI r_CATG'y
Cuts at: 0 32 1035
Size: 32 1003
PfoI T'CCnGG_A
Cuts at: 0 908 1035
Size: 908 127
PpuMI rG'GwC_Cy
Cuts at: 0 21 906 1035
Size: 21 885 129
Fragments arranged by size:
885 129 21
PstI C_TGCA'G
Cuts at: 0 941 1035
Size: 941 94
PvuII CAG'CTG
Cuts at: 0 957 999 1035
Size: 957 42 36
Fragments arranged by size:
957 42 36
SacI G_AGCT'C
Cuts at: 0 518 917 971 1035
Size: 518 399 54 64
Fragments arranged by size:
518 399 64 54
SacII CC_GC'GG
Cuts at: 0 140 152 545 1035
Size: 140 12 393 490
Fragments arranged by size:
490 393 140 12
SanDI GG'GwC_CC
Cuts at: 0 21 1035
Size: 21 1014
SfcI C'TryA_G
Cuts at: 0 937 1035
Size: 937 98
SfoI GGC'GCC
Cuts at: 0 143 266 430 575 728 764 1035
Size: 143 123 164 145 153 36 271
Fragments arranged by size:
271 164 153 145 143 123 36
SgrAI Cr'CCGG_yG
Cuts at: 0 178 759 1035
Size: 178 581 276
Fragments arranged by size:
581 276 178
SmaI CCC'GGG
Cuts at: 0 162 1035
Size: 162 873
SmlI C'TyrA_G
Cuts at: 0 517 1035
Size: 517 518
StyI C'CwwG_G
Cuts at: 0 68 443 722 1035
Size: 68 375 279 313
Fragments arranged by size:
375 313 279 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 1035
Size: 497 538
TatI w'GTAC_w
Cuts at: 0 818 1035
Size: 818 217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 795 1035
Size: 795 240
XmaI C'CCGG_G
Cuts at: 0 160 1035
Size: 160 875
XmnI GAAnn'nnTTC
Cuts at: 0 988 1035
Size: 988 47
Enzymes that do cut:
AcuI AscI AvaI BanI BanII BbeI BbsI BcgI
BglI BlpI BmgBI BpmI BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI DraI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PfoI PpuMI PstI PvuII SacI SacII SanDI SfcI
SfoI SgrAI SmaI SmlI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglII Bme1580I BmrI BmtI BplI Bpu10I
BsaI BsaBI BsaWI BsmI BspEI BspHI BsrDI BsrGI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraIII DrdI
EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI ZraI