Restriction map for
pF1KB9578
(Linear) (Six-base) MAPSORT of: KIBB9578.seq Check: 2758 from: 1 to: 711
KIBB9578.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 711
Size: 300 411
AfeI AGC'GCT
Cuts at: 0 271 711
Size: 271 440
AlwNI CAG_nnn'CTG
Cuts at: 0 519 522 711
Size: 519 3 189
Fragments arranged by size:
519 189 3
AscI GG'CGCG_CC
Cuts at: 0 364 711
Size: 364 347
AvaI C'yCGr_G
Cuts at: 0 673 711
Size: 673 38
BanI G'GyrC_C
Cuts at: 0 31 189 711
Size: 31 158 522
Fragments arranged by size:
522 158 31
BanII G_rGCy'C
Cuts at: 0 655 711
Size: 655 56
BbeI G_GCGC'C
Cuts at: 0 35 193 711
Size: 35 158 518
Fragments arranged by size:
518 158 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 711
Size: 269 442
BglI GCCn_nnn'nGGC
Cuts at: 0 115 133 361 556 711
Size: 115 18 228 195 155
Fragments arranged by size:
228 195 155 115 18
BlpI GC'TnA_GC
Cuts at: 0 667 711
Size: 667 44
BmgBI CAC'GTC
Cuts at: 0 435 711
Size: 435 276
BsaI GGTCTCn'nnnn_
Cuts at: 0 195 633 711
Size: 195 438 78
Fragments arranged by size:
438 195 78
BsaHI Gr'CG_yC
Cuts at: 0 32 190 563 711
Size: 32 158 373 148
Fragments arranged by size:
373 158 148 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 711
Size: 592 30 89
Fragments arranged by size:
592 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 691 711
Size: 691 20
BseYI C'CCAG_C
Cuts at: 0 47 59 711
Size: 47 12 652
Fragments arranged by size:
652 47 12
BsiEI CG_ry'CG
Cuts at: 0 104 119 356 494 711
Size: 104 15 237 138 217
Fragments arranged by size:
237 217 138 104 15
BsiHKAI G_wGCw'C
Cuts at: 0 434 711
Size: 434 277
Bsp1286I G_dGCh'C
Cuts at: 0 434 655 711
Size: 434 221 56
Fragments arranged by size:
434 221 56
BsrBI CCG'CTC
Cuts at: 0 26 666 711
Size: 26 640 45
Fragments arranged by size:
640 45 26
BsrFI r'CCGG_y
Cuts at: 0 34 204 305 351 616 624 711
Size: 34 170 101 46 265 8 87
Fragments arranged by size:
265 170 101 87 46 34 8
BssHII G'CGCG_C
Cuts at: 0 146 364 506 711
Size: 146 218 142 205
Fragments arranged by size:
218 205 146 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 711
Size: 519 192
Bsu36I CC'TnA_GG
Cuts at: 0 225 711
Size: 225 486
BtgI C'CryG_G
Cuts at: 0 98 110 359 611 711
Size: 98 12 249 252 100
Fragments arranged by size:
252 249 100 98 12
BtsI GCAGTG_nn'
Cuts at: 0 478 711
Size: 478 233
EaeI y'GGCC_r
Cuts at: 0 36 101 116 216 353 449 614 711
Size: 36 65 15 100 137 96 165 97
Fragments arranged by size:
165 137 100 97 96 65 36 15
EagI C'GGCC_G
Cuts at: 0 101 116 353 711
Size: 101 15 237 358
Fragments arranged by size:
358 237 101 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 711
Size: 300 411
HaeII r_GCGC'y
Cuts at: 0 35 193 250 273 548 711
Size: 35 158 57 23 275 163
Fragments arranged by size:
275 163 158 57 35 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 637 669 711
Size: 592 32 13 32 42
Fragments arranged by size:
592 42 32 32 13
HincII GTy'rAC
Cuts at: 0 405 711
Size: 405 306
KasI G'GCGC_C
Cuts at: 0 31 189 711
Size: 31 158 522
Fragments arranged by size:
522 158 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 623 711
Size: 620 3 88
Fragments arranged by size:
620 88 3
MspA1I CmG'CkG
Cuts at: 0 112 133 198 320 519 711
Size: 112 21 65 122 199 192
Fragments arranged by size:
199 192 122 112 65 21
NaeI GCC'GGC
Cuts at: 0 36 307 353 618 711
Size: 36 271 46 265 93
Fragments arranged by size:
271 265 93 46 36
NarI GG'CG_CC
Cuts at: 0 32 190 711
Size: 32 158 521
Fragments arranged by size:
521 158 32
NcoI C'CATG_G
Cuts at: 0 611 711
Size: 611 100
NgoMIV G'CCGG_C
Cuts at: 0 34 305 351 616 711
Size: 34 271 46 265 95
Fragments arranged by size:
271 265 95 46 34
NotI GC'GGCC_GC
Cuts at: 0 116 711
Size: 116 595
PstI C_TGCA'G
Cuts at: 0 515 711
Size: 515 196
PvuII CAG'CTG
Cuts at: 0 198 519 711
Size: 198 321 192
Fragments arranged by size:
321 198 192
SacII CC_GC'GG
Cuts at: 0 113 711
Size: 113 598
SfcI C'TryA_G
Cuts at: 0 330 511 711
Size: 330 181 200
Fragments arranged by size:
330 200 181
SfoI GGC'GCC
Cuts at: 0 33 191 711
Size: 33 158 520
Fragments arranged by size:
520 158 33
StyI C'CwwG_G
Cuts at: 0 611 711
Size: 611 100
TatI w'GTAC_w
Cuts at: 0 497 711
Size: 497 214
Enzymes that do cut:
AcuI AfeI AlwNI AscI AvaI BanI BanII BbeI
BbsI BglI BlpI BmgBI BsaI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstAPI
Bsu36I BtgI BtsI EaeI EagI Eco57MI HaeII Hin4I
HincII KasI MmeI MspA1I NaeI NarI NcoI NgoMIV
NotI PstI PvuII SacII SfcI SfoI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MscI MslI NdeI NheI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI