Restriction map for
pF1KB9575
(Linear) (Six-base) MAPSORT of: KIBB9575.seq Check: 4441 from: 1 to: 1821
KIBB9575.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 833 866 1821
Size: 833 33 955
Fragments arranged by size:
955 833 33
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 927 1480 1540 1819 1821
Size: 494 433 553 60 279 2
Fragments arranged by size:
553 494 433 279 60 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 245 566 1247 1821
Size: 245 321 681 574
Fragments arranged by size:
681 574 321 245
AlwNI CAG_nnn'CTG
Cuts at: 0 1458 1821
Size: 1458 363
ApaLI G'TGCA_C
Cuts at: 0 1420 1821
Size: 1420 401
ApoI r'AATT_y
Cuts at: 0 1663 1774 1821
Size: 1663 111 47
Fragments arranged by size:
1663 111 47
AvaI C'yCGr_G
Cuts at: 0 42 466 1821
Size: 42 424 1355
Fragments arranged by size:
1355 424 42
BamHI G'GATC_C
Cuts at: 0 1589 1821
Size: 1589 232
BanII G_rGCy'C
Cuts at: 0 1445 1821
Size: 1445 376
BbsI GAAGACnn'nnnn_
Cuts at: 0 501 1125 1596 1821
Size: 501 624 471 225
Fragments arranged by size:
624 501 471 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 662 1821
Size: 628 34 1159
Fragments arranged by size:
1159 628 34
BclI T'GATC_A
Cuts at: 0 281 1821
Size: 281 1540
BfrBI ATG'CAT
Cuts at: 0 1230 1821
Size: 1230 591
BglI GCCn_nnn'nGGC
Cuts at: 0 1552 1821
Size: 1552 269
Bme1580I G_kGCm'C
Cuts at: 0 1406 1424 1821
Size: 1406 18 397
Fragments arranged by size:
1406 397 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1030 1276 1821
Size: 351 679 246 545
Fragments arranged by size:
679 545 351 246
BsaI GGTCTCn'nnnn_
Cuts at: 0 1809 1821
Size: 1809 12
BsaAI yAC'GTr
Cuts at: 0 1190 1821
Size: 1190 631
BsaBI GATnn'nnATC
Cuts at: 0 1714 1821
Size: 1714 107
BsaWI w'CCGG_w
Cuts at: 0 25 1586 1821
Size: 25 1561 235
Fragments arranged by size:
1561 235 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 1821
Size: 571 30 1220
Fragments arranged by size:
1220 571 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 772 1138 1795 1821
Size: 769 3 366 657 26
Fragments arranged by size:
769 657 366 26 3
BseYI C'CCAG_C
Cuts at: 0 187 898 1493 1821
Size: 187 711 595 328
Fragments arranged by size:
711 595 328 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1821
Size: 952 869
BsiHKAI G_wGCw'C
Cuts at: 0 113 1424 1821
Size: 113 1311 397
Fragments arranged by size:
1311 397 113
BsmI GAATG_Cn'
Cuts at: 0 1283 1821
Size: 1283 538
Bsp1286I G_dGCh'C
Cuts at: 0 113 1406 1424 1445 1821
Size: 113 1293 18 21 376
Fragments arranged by size:
1293 376 113 21 18
BspEI T'CCGG_A
Cuts at: 0 1586 1821
Size: 1586 235
BsrBI CCG'CTC
Cuts at: 0 87 1821
Size: 87 1734
BsrDI GCAATG_nn'
Cuts at: 0 479 504 1244 1821
Size: 479 25 740 577
Fragments arranged by size:
740 577 479 25
BssSI C'ACGA_G
Cuts at: 0 1627 1821
Size: 1627 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1236 1821
Size: 1236 585
BstEII G'GTnAC_C
Cuts at: 0 880 1065 1339 1821
Size: 880 185 274 482
Fragments arranged by size:
880 482 274 185
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1542 1821
Size: 1542 279
BstYI r'GATC_y
Cuts at: 0 213 398 1589 1766 1821
Size: 213 185 1191 177 55
Fragments arranged by size:
1191 213 185 177 55
BstZ17I GTA'TAC
Cuts at: 0 867 1821
Size: 867 954
Bsu36I CC'TnA_GG
Cuts at: 0 66 275 1821
Size: 66 209 1546
Fragments arranged by size:
1546 209 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1701 1821
Size: 1701 120
ClaI AT'CG_AT
Cuts at: 0 689 1821
Size: 689 1132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 543 578 1821
Size: 543 35 1243
Fragments arranged by size:
1243 543 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1348 1729 1821
Size: 1348 381 92
Fragments arranged by size:
1348 381 92
EaeI y'GGCC_r
Cuts at: 0 2 1241 1544 1821
Size: 2 1239 303 277
Fragments arranged by size:
1239 303 277 2
EarI CTCTTCn'nnn_
Cuts at: 0 622 1027 1821
Size: 622 405 794
Fragments arranged by size:
794 622 405
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 952 1821
Size: 952 869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 494 927 1030 1276 1480 1540 1819
Size: 351 143 433 103 246 204 60 279
Cuts at: 1819 1821
Size: 2
Fragments arranged by size:
433 351 279 246 204 143 103 60
2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 16 1821
Size: 16 1805
EcoO109I rG'GnC_Cy
Cuts at: 0 225 568 1821
Size: 225 343 1253
Fragments arranged by size:
1253 343 225
FspI TGC'GCA
Cuts at: 0 1327 1821
Size: 1327 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 1092 1124 1821
Size: 139 32 921 32 697
Fragments arranged by size:
921 697 139 32 32
HincII GTy'rAC
Cuts at: 0 1686 1821
Size: 1686 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 866 1821
Size: 839 27 955
Fragments arranged by size:
955 839 27
MscI TGG'CCA
Cuts at: 0 4 1243 1546 1821
Size: 4 1239 303 275
Fragments arranged by size:
1239 303 275 4
MslI CAynn'nnrTG
Cuts at: 0 548 1003 1534 1540 1821
Size: 548 455 531 6 281
Fragments arranged by size:
548 531 455 281 6
MspA1I CmG'CkG
Cuts at: 0 142 422 731 1296 1458 1640 1821
Size: 142 280 309 565 162 182 181
Fragments arranged by size:
565 309 280 182 181 162 142
NsiI A_TGCA'T
Cuts at: 0 1232 1821
Size: 1232 589
NspI r_CATG'y
Cuts at: 0 1176 1234 1332 1821
Size: 1176 58 98 489
Fragments arranged by size:
1176 489 98 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 493 671 791 1821
Size: 493 178 120 1030
Fragments arranged by size:
1030 493 178 120
PfoI T'CCnGG_A
Cuts at: 0 401 1562 1821
Size: 401 1161 259
Fragments arranged by size:
1161 401 259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 1821
Size: 1567 32 222
Fragments arranged by size:
1567 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 225 568 1821
Size: 225 343 1253
Fragments arranged by size:
1253 343 225
PstI C_TGCA'G
Cuts at: 0 1454 1821
Size: 1454 367
PvuII CAG'CTG
Cuts at: 0 422 1296 1458 1821
Size: 422 874 162 363
Fragments arranged by size:
874 422 363 162
SfcI C'TryA_G
Cuts at: 0 349 537 1397 1450 1821
Size: 349 188 860 53 371
Fragments arranged by size:
860 371 349 188 53
SphI G_CATG'C
Cuts at: 0 1234 1332 1821
Size: 1234 98 489
Fragments arranged by size:
1234 489 98
StuI AGG'CCT
Cuts at: 0 11 1821
Size: 11 1810
StyI C'CwwG_G
Cuts at: 0 460 575 1727 1821
Size: 460 115 1152 94
Fragments arranged by size:
1152 460 115 94
TatI w'GTAC_w
Cuts at: 0 1479 1821
Size: 1479 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 1821
Size: 198 32 1591
Fragments arranged by size:
1591 198 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1005 1539 1821
Size: 1005 534 282
Fragments arranged by size:
1005 534 282
XmnI GAAnn'nnTTC
Cuts at: 0 21 1821
Size: 21 1800
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaLI ApoI AvaI BamHI
BanII BbsI BcgI BclI BfrBI BglI Bme1580I BpmI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI
Hin4I HincII MmeI MscI MslI MspA1I NsiI NspI
PflMI PfoI PpiI PpuMI PstI PvuII SfcI SphI
StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaHI BsiEI BsiWI
BsmBI BspHI BspMI BsrFI BsrGI BssHII BstBI BtgI
BtsI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI
FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI