Restriction map for
pF1KB9569
(Linear) (Six-base) MAPSORT of: KIBB9569.seq Check: 953 from: 1 to: 2223
KIBB9569.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 939 1469 2223
Size: 251 688 530 754
Fragments arranged by size:
754 688 530 251
AflIII A'CryG_T
Cuts at: 0 1007 2223
Size: 1007 1216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 449 452 2223
Size: 449 3 1771
Fragments arranged by size:
1771 449 3
AlwNI CAG_nnn'CTG
Cuts at: 0 390 1714 1717 1777 2089 2223
Size: 390 1324 3 60 312 134
Fragments arranged by size:
1324 390 312 134 60 3
ApoI r'AATT_y
Cuts at: 0 1098 2223
Size: 1098 1125
BanI G'GyrC_C
Cuts at: 0 1803 1828 2223
Size: 1803 25 395
Fragments arranged by size:
1803 395 25
BanII G_rGCy'C
Cuts at: 0 839 926 1995 2223
Size: 839 87 1069 228
Fragments arranged by size:
1069 839 228 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 2223
Size: 10 2213
BbvCI CC'TCA_GC
Cuts at: 0 680 994 1542 1584 2223
Size: 680 314 548 42 639
Fragments arranged by size:
680 639 548 314 42
BclI T'GATC_A
Cuts at: 0 1233 2223
Size: 1233 990
BglI GCCn_nnn'nGGC
Cuts at: 0 2185 2215 2223
Size: 2185 30 8
Fragments arranged by size:
2185 30 8
BglII A'GATC_T
Cuts at: 0 17 2223
Size: 17 2206
Bme1580I G_kGCm'C
Cuts at: 0 1095 2223
Size: 1095 1128
BmgBI CAC'GTC
Cuts at: 0 1553 2223
Size: 1553 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 332 833 1970 2223
Size: 332 501 1137 253
Fragments arranged by size:
1137 501 332 253
BmtI G_CTAG'C
Cuts at: 0 307 2223
Size: 307 1916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1077 1883 2223
Size: 1077 806 340
Fragments arranged by size:
1077 806 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 346 461 1065 1088 1517 1844 2223
Size: 329 17 115 604 23 429 327 379
Fragments arranged by size:
604 429 379 329 327 115 23 17
Bpu10I CC'TnA_GC
Cuts at: 0 680 994 1542 1584 1858 2223
Size: 680 314 548 42 274 365
Fragments arranged by size:
680 548 365 314 274 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 877 1140 2223
Size: 868 9 263 1083
Fragments arranged by size:
1083 868 263 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 649 1147 1612 2076 2223
Size: 649 498 465 464 147
Fragments arranged by size:
649 498 465 464 147
BsaBI GATnn'nnATC
Cuts at: 0 16 2223
Size: 16 2207
BsaHI Gr'CG_yC
Cuts at: 0 337 625 2223
Size: 337 288 1598
Fragments arranged by size:
1598 337 288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1480 1510 1515 1545 1629 1659 2113 2143
Size: 1480 30 5 30 84 30 454 30
Cuts at: 2143 2223
Size: 80
Fragments arranged by size:
1480 454 84 80 30 30 30 30
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 116 847 865 2003 2223
Size: 116 731 18 1138 220
Fragments arranged by size:
1138 731 220 116 18
BseYI C'CCAG_C
Cuts at: 0 257 293 344 734 823 1357 1875 2223
Size: 257 36 51 390 89 534 518 348
Fragments arranged by size:
534 518 390 348 257 89 51 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 424 1427 1718 2087 2223
Size: 292 132 1003 291 369 136
Fragments arranged by size:
1003 369 292 291 136 132
BsiHKAI G_wGCw'C
Cuts at: 0 1869 2223
Size: 1869 354
Bsp1286I G_dGCh'C
Cuts at: 0 839 926 1095 1869 1995 2223
Size: 839 87 169 774 126 228
Fragments arranged by size:
839 774 228 169 126 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 566 2223
Size: 566 1657
BsrBI CCG'CTC
Cuts at: 0 1862 2223
Size: 1862 361
BsrGI T'GTAC_A
Cuts at: 0 201 2223
Size: 201 2022
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 1609 1714 1717 1777 2089 2223
Size: 90 1519 105 3 60 312 134
Fragments arranged by size:
1519 312 134 105 90 60 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 163 2223
Size: 69 94 2060
Fragments arranged by size:
2060 94 69
BstYI r'GATC_y
Cuts at: 0 17 482 516 2223
Size: 17 465 34 1707
Fragments arranged by size:
1707 465 34 17
BtgI C'CryG_G
Cuts at: 0 1766 2223
Size: 1766 457
BtsI GCAGTG_nn'
Cuts at: 0 407 1323 1410 2223
Size: 407 916 87 813
Fragments arranged by size:
916 813 407 87
DraI TTT'AAA
Cuts at: 0 197 2223
Size: 197 2026
EarI CTCTTCn'nnn_
Cuts at: 0 1174 1208 1940 2167 2223
Size: 1174 34 732 227 56
Fragments arranged by size:
1174 732 227 56 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 405 444 2223
Size: 405 39 1779
Fragments arranged by size:
1779 405 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 329 346 461 939 1065 1088 1469
Size: 251 78 17 115 478 126 23 381
Cuts at: 1469 1517 1844 2223
Size: 48 327 379
Fragments arranged by size:
478 381 379 327 251 126 115 78
48 23 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 2223
Size: 98 2125
EcoO109I rG'GnC_Cy
Cuts at: 0 1853 2223
Size: 1853 370
EcoRI G'AATT_C
Cuts at: 0 1098 2223
Size: 1098 1125
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 269 1203 2223
Size: 269 934 1020
Fragments arranged by size:
1020 934 269
HaeII r_GCGC'y
Cuts at: 0 1531 2223
Size: 1531 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 434 466 1218 1250 1480 1512
Size: 92 32 310 32 752 32 230 32
Cuts at: 1512 1998 2030 2223
Size: 486 32 193
Fragments arranged by size:
752 486 310 230 193 92 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 800 2223
Size: 800 1423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 607 1523 2223
Size: 180 427 916 700
Fragments arranged by size:
916 700 427 180
MslI CAynn'nnrTG
Cuts at: 0 472 1165 2223
Size: 472 693 1058
Fragments arranged by size:
1058 693 472
MspA1I CmG'CkG
Cuts at: 0 387 1714 1774 2183 2223
Size: 387 1327 60 409 40
Fragments arranged by size:
1327 409 387 60 40
NcoI C'CATG_G
Cuts at: 0 1766 2223
Size: 1766 457
NheI G'CTAG_C
Cuts at: 0 303 2223
Size: 303 1920
NspI r_CATG'y
Cuts at: 0 1011 2223
Size: 1011 1212
PasI CC'CwG_GG
Cuts at: 0 630 2223
Size: 630 1593
PciI A'CATG_T
Cuts at: 0 1007 2223
Size: 1007 1216
PflMI CCAn_nnn'nTGG
Cuts at: 0 1318 1680 2223
Size: 1318 362 543
Fragments arranged by size:
1318 543 362
PfoI T'CCnGG_A
Cuts at: 0 1104 2223
Size: 1104 1119
PpuMI rG'GwC_Cy
Cuts at: 0 1853 2223
Size: 1853 370
PstI C_TGCA'G
Cuts at: 0 402 1782 2094 2223
Size: 402 1380 312 129
Fragments arranged by size:
1380 402 312 129
PvuII CAG'CTG
Cuts at: 0 387 1714 1774 2223
Size: 387 1327 60 449
Fragments arranged by size:
1327 449 387 60
SanDI GG'GwC_CC
Cuts at: 0 1853 2223
Size: 1853 370
SexAI A'CCwGG_T
Cuts at: 0 1967 2223
Size: 1967 256
SfcI C'TryA_G
Cuts at: 0 95 398 703 1778 2090 2108 2223
Size: 95 303 305 1075 312 18 115
Fragments arranged by size:
1075 312 305 303 115 95 18
SmlI C'TyrA_G
Cuts at: 0 847 856 1119 2223
Size: 847 9 263 1104
Fragments arranged by size:
1104 847 263 9
StyI C'CwwG_G
Cuts at: 0 135 1766 2213 2223
Size: 135 1631 447 10
Fragments arranged by size:
1631 447 135 10
TatI w'GTAC_w
Cuts at: 0 201 1894 2223
Size: 201 1693 329
Fragments arranged by size:
1693 329 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1515 1547 1629 1661 2113 2145 2223
Size: 1515 32 82 32 452 32 78
Fragments arranged by size:
1515 452 82 78 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1164 1677 2223
Size: 1164 513 546
Fragments arranged by size:
1164 546 513
XmnI GAAnn'nnTTC
Cuts at: 0 1183 2223
Size: 1183 1040
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI BanI BanII BbsI
BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrGI
BstAPI BstXI BstYI BtgI BtsI DraI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
MmeI MslI MspA1I NcoI NheI NspI PasI PciI
PflMI PfoI PpuMI PstI PvuII SanDI SexAI SfcI
SmlI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BlpI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI