Restriction map for
pF1KB9560
(Linear) (Six-base) MAPSORT of: KIBB9560.seq Check: 9686 from: 1 to: 3408
KIBB9560.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 1850 2237 3408
Size: 1850 387 1171
Fragments arranged by size:
1850 1171 387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1512 1884 2338 2425 2565 2572 2605
Size: 668 844 372 454 87 140 7 33
Cuts at: 2605 2990 3177 3408
Size: 385 187 231
Fragments arranged by size:
844 668 454 385 372 231 187 140
87 33 7
AfeI AGC'GCT
Cuts at: 0 112 2332 3408
Size: 112 2220 1076
Fragments arranged by size:
2220 1076 112
AflII C'TTAA_G
Cuts at: 0 667 3408
Size: 667 2741
AflIII A'CryG_T
Cuts at: 0 1505 3364 3408
Size: 1505 1859 44
Fragments arranged by size:
1859 1505 44
AgeI A'CCGG_T
Cuts at: 0 173 3408
Size: 173 3235
AhdI GACnn_n'nnGTC
Cuts at: 0 3060 3408
Size: 3060 348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2537 3408
Size: 2537 871
AlwNI CAG_nnn'CTG
Cuts at: 0 1411 2524 3408
Size: 1411 1113 884
Fragments arranged by size:
1411 1113 884
ApoI r'AATT_y
Cuts at: 0 225 517 747 923 2015 2303 2575 3408
Size: 225 292 230 176 1092 288 272 833
Fragments arranged by size:
1092 833 292 288 272 230 225 176
AseI AT'TA_AT
Cuts at: 0 1211 3408
Size: 1211 2197
AvaI C'yCGr_G
Cuts at: 0 578 869 3408
Size: 578 291 2539
Fragments arranged by size:
2539 578 291
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3092 3125 3408
Size: 3092 33 283
Fragments arranged by size:
3092 283 33
BamHI G'GATC_C
Cuts at: 0 1532 3408
Size: 1532 1876
BanI G'GyrC_C
Cuts at: 0 640 1850 2237 3408
Size: 640 1210 387 1171
Fragments arranged by size:
1210 1171 640 387
BanII G_rGCy'C
Cuts at: 0 244 656 3121 3163 3408
Size: 244 412 2465 42 245
Fragments arranged by size:
2465 412 245 244 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 2096 3408
Size: 2096 1312
BclI T'GATC_A
Cuts at: 0 945 3201 3408
Size: 945 2256 207
Fragments arranged by size:
2256 945 207
BmgBI CAC'GTC
Cuts at: 0 1508 3408
Size: 1508 1900
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 850 3408
Size: 329 521 2558
Fragments arranged by size:
2558 521 329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3029 3205 3408
Size: 3029 176 203
Fragments arranged by size:
3029 203 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2131 3408
Size: 2131 1277
Bpu10I CC'TnA_GC
Cuts at: 0 1028 3408
Size: 1028 2380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 694 2685 3408
Size: 88 606 1991 723
Fragments arranged by size:
1991 723 606 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 1411 1601 3408
Size: 79 1332 190 1807
Fragments arranged by size:
1807 1332 190 79
BsaAI yAC'GTr
Cuts at: 0 2560 3408
Size: 2560 848
BsaBI GATnn'nnATC
Cuts at: 0 2056 3408
Size: 2056 1352
BsaWI w'CCGG_w
Cuts at: 0 173 3408
Size: 173 3235
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 386 416 3408
Size: 386 30 2992
Fragments arranged by size:
2992 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 477 560 646 1288 2425 2512 3408
Size: 97 380 83 86 642 1137 87 896
Fragments arranged by size:
1137 896 642 380 97 87 86 83
BsiHKAI G_wGCw'C
Cuts at: 0 656 1489 3121 3408
Size: 656 833 1632 287
Fragments arranged by size:
1632 833 656 287
BsmI GAATG_Cn'
Cuts at: 0 771 967 3408
Size: 771 196 2441
Fragments arranged by size:
2441 771 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1514 2391 3391 3408
Size: 1514 877 1000 17
Fragments arranged by size:
1514 1000 877 17
Bsp1286I G_dGCh'C
Cuts at: 0 244 656 1489 3121 3163 3408
Size: 244 412 833 1632 42 245
Fragments arranged by size:
1632 833 412 245 244 42
BspHI T'CATG_A
Cuts at: 0 795 3408
Size: 795 2613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 1064 3408
Size: 680 384 2344
Fragments arranged by size:
2344 680 384
BsrDI GCAATG_nn'
Cuts at: 0 2354 2963 3408
Size: 2354 609 445
Fragments arranged by size:
2354 609 445
BsrFI r'CCGG_y
Cuts at: 0 173 937 3408
Size: 173 764 2471
Fragments arranged by size:
2471 764 173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3064 3349 3408
Size: 3064 285 59
Fragments arranged by size:
3064 285 59
BstYI r'GATC_y
Cuts at: 0 277 1532 2461 3408
Size: 277 1255 929 947
Fragments arranged by size:
1255 947 929 277
Bsu36I CC'TnA_GG
Cuts at: 0 2095 3408
Size: 2095 1313
BtgI C'CryG_G
Cuts at: 0 1730 2021 3408
Size: 1730 291 1387
Fragments arranged by size:
1730 1387 291
BtsI GCAGTG_nn'
Cuts at: 0 836 3204 3408
Size: 836 2368 204
Fragments arranged by size:
2368 836 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1606 1641 3408
Size: 1606 35 1767
Fragments arranged by size:
1767 1606 35
DraI TTT'AAA
Cuts at: 0 528 3408
Size: 528 2880
EaeI y'GGCC_r
Cuts at: 0 2 716 1370 1733 2120 3218 3408
Size: 2 714 654 363 387 1098 190
Fragments arranged by size:
1098 714 654 387 363 190 2
EarI CTCTTCn'nnn_
Cuts at: 0 644 1259 1379 2810 2845 2998 3196 3408
Size: 644 615 120 1431 35 153 198 212
Fragments arranged by size:
1431 644 615 212 198 153 120 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2247 3408
Size: 2247 1161
EcoICRI GAG'CTC
Cuts at: 0 654 3119 3408
Size: 654 2465 289
Fragments arranged by size:
2465 654 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1512 1884 2131 2338 2425 2565 2572
Size: 668 844 372 247 207 87 140 7
Cuts at: 2572 2605 2990 3177 3408
Size: 33 385 187 231
Fragments arranged by size:
844 668 385 372 247 231 207 187
140 87 33 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1706 2211 3154 3408
Size: 1706 505 943 254
Fragments arranged by size:
1706 943 505 254
EcoO109I rG'GnC_Cy
Cuts at: 0 1995 2375 3408
Size: 1995 380 1033
Fragments arranged by size:
1995 1033 380
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 129 439 3408
Size: 129 310 2969
Fragments arranged by size:
2969 310 129
FspI TGC'GCA
Cuts at: 0 2225 3408
Size: 2225 1183
HaeII r_GCGC'y
Cuts at: 0 114 2334 3408
Size: 114 2220 1074
Fragments arranged by size:
2220 1074 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 3046 3078 3408
Size: 472 32 2542 32 330
Fragments arranged by size:
2542 472 330 32 32
HincII GTy'rAC
Cuts at: 0 165 1581 3408
Size: 165 1416 1827
Fragments arranged by size:
1827 1416 165
HindIII A'AGCT_T
Cuts at: 0 355 449 670 3408
Size: 355 94 221 2738
Fragments arranged by size:
2738 355 221 94
HpaI GTT'AAC
Cuts at: 0 165 3408
Size: 165 3243
KpnI G_GTAC'C
Cuts at: 0 1854 2241 3408
Size: 1854 387 1167
Fragments arranged by size:
1854 1167 387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 2269 3408
Size: 458 1811 1139
Fragments arranged by size:
1811 1139 458
MscI TGG'CCA
Cuts at: 0 4 1372 1735 2122 3220 3408
Size: 4 1368 363 387 1098 188
Fragments arranged by size:
1368 1098 387 363 188 4
MslI CAynn'nnrTG
Cuts at: 0 1741 3408
Size: 1741 1667
MspA1I CmG'CkG
Cuts at: 0 379 630 1523 3408
Size: 379 251 893 1885
Fragments arranged by size:
1885 893 379 251
NspI r_CATG'y
Cuts at: 0 2431 2518 3368 3408
Size: 2431 87 850 40
Fragments arranged by size:
2431 850 87 40
PciI A'CATG_T
Cuts at: 0 3364 3408
Size: 3364 44
PfoI T'CCnGG_A
Cuts at: 0 443 3408
Size: 443 2965
PpuMI rG'GwC_Cy
Cuts at: 0 1995 2375 3408
Size: 1995 380 1033
Fragments arranged by size:
1995 1033 380
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 410 442 3408
Size: 410 32 2966
Fragments arranged by size:
2966 410 32
PvuII CAG'CTG
Cuts at: 0 379 630 1523 3408
Size: 379 251 893 1885
Fragments arranged by size:
1885 893 379 251
SacI G_AGCT'C
Cuts at: 0 656 3121 3408
Size: 656 2465 287
Fragments arranged by size:
2465 656 287
SanDI GG'GwC_CC
Cuts at: 0 2375 3408
Size: 2375 1033
SapI GCTCTTCn'nnn_
Cuts at: 0 644 1259 3408
Size: 644 615 2149
Fragments arranged by size:
2149 644 615
SexAI A'CCwGG_T
Cuts at: 0 1562 3408
Size: 1562 1846
SfcI C'TryA_G
Cuts at: 0 1068 1703 1753 3408
Size: 1068 635 50 1655
Fragments arranged by size:
1655 1068 635 50
SmlI C'TyrA_G
Cuts at: 0 103 667 673 869 2700 3408
Size: 103 564 6 196 1831 708
Fragments arranged by size:
1831 708 564 196 103 6
SnaBI TAC'GTA
Cuts at: 0 2560 3408
Size: 2560 848
SphI G_CATG'C
Cuts at: 0 2518 3408
Size: 2518 890
SspI AAT'ATT
Cuts at: 0 3270 3408
Size: 3270 138
StuI AGG'CCT
Cuts at: 0 1698 1909 3408
Size: 1698 211 1499
Fragments arranged by size:
1698 1499 211
StyI C'CwwG_G
Cuts at: 0 2889 3104 3408
Size: 2889 215 304
Fragments arranged by size:
2889 304 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1138 1468 3408
Size: 1138 330 1940
Fragments arranged by size:
1940 1138 330
TatI w'GTAC_w
Cuts at: 0 3007 3408
Size: 3007 401
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1372 1404 3408
Size: 1372 32 2004
Fragments arranged by size:
2004 1372 32
XbaI T'CTAG_A
Cuts at: 0 490 882 957 3408
Size: 490 392 75 2451
Fragments arranged by size:
2451 490 392 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 777 1543 1986 3408
Size: 441 336 766 443 1422
Fragments arranged by size:
1422 766 443 441 336
XhoI C'TCGA_G
Cuts at: 0 869 3408
Size: 869 2539
XmnI GAAnn'nnTTC
Cuts at: 0 2578 3408
Size: 2578 830
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AgeI AhdI AlfI
AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BclI BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NspI PciI PfoI PpuMI PsrI PvuII SacI SanDI
SapI SexAI SfcI SmlI SnaBI SphI SspI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AleI AloI ApaI ApaLI
AscI AsiSI AvrII BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmtI BsaHI BseYI BsgI
BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI DraIII DrdI EagI
EcoRI EcoRV FseI FspAI KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
SwaI Tth111I XmaI ZraI