Restriction map for
pF1KB9551
(Linear) (Six-base) MAPSORT of: KIBB9551.seq Check: 353 from: 1 to: 1155
KIBB9551.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 393 1155
Size: 393 762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 966 1155
Size: 904 62 189
Fragments arranged by size:
904 189 62
AflIII A'CryG_T
Cuts at: 0 845 1155
Size: 845 310
AleI CACnn'nnGTG
Cuts at: 0 259 1155
Size: 259 896
AlwNI CAG_nnn'CTG
Cuts at: 0 597 1075 1155
Size: 597 478 80
Fragments arranged by size:
597 478 80
AvaI C'yCGr_G
Cuts at: 0 547 1155
Size: 547 608
BanI G'GyrC_C
Cuts at: 0 393 1046 1155
Size: 393 653 109
Fragments arranged by size:
653 393 109
BanII G_rGCy'C
Cuts at: 0 347 1155
Size: 347 808
BbsI GAAGACnn'nnnn_
Cuts at: 0 976 1155
Size: 976 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 1155
Size: 9 34 1112
Fragments arranged by size:
1112 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 232 1155
Size: 232 923
BglI GCCn_nnn'nGGC
Cuts at: 0 1141 1155
Size: 1141 14
BlpI GC'TnA_GC
Cuts at: 0 16 955 1155
Size: 16 939 200
Fragments arranged by size:
939 200 16
Bme1580I G_kGCm'C
Cuts at: 0 1051 1155
Size: 1051 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 171 781 1078 1155
Size: 76 95 610 297 77
Fragments arranged by size:
610 297 95 77 76
BsaBI GATnn'nnATC
Cuts at: 0 1015 1155
Size: 1015 140
BsaHI Gr'CG_yC
Cuts at: 0 605 640 1128 1155
Size: 605 35 488 27
Fragments arranged by size:
605 488 35 27
BsaWI w'CCGG_w
Cuts at: 0 1019 1155
Size: 1019 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 889 919 1155
Size: 754 30 105 30 236
Fragments arranged by size:
754 236 105 30 30
BseYI C'CCAG_C
Cuts at: 0 516 900 1155
Size: 516 384 255
Fragments arranged by size:
516 384 255
BsiHKAI G_wGCw'C
Cuts at: 0 895 1155
Size: 895 260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 596 1155
Size: 596 559
Bsp1286I G_dGCh'C
Cuts at: 0 347 895 1051 1155
Size: 347 548 156 104
Fragments arranged by size:
548 347 156 104
BspEI T'CCGG_A
Cuts at: 0 1019 1155
Size: 1019 136
BsrGI T'GTAC_A
Cuts at: 0 128 1155
Size: 128 1027
BssSI C'ACGA_G
Cuts at: 0 1113 1155
Size: 1113 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 597 1155
Size: 597 558
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 1155
Size: 570 585
BstYI r'GATC_y
Cuts at: 0 1091 1155
Size: 1091 64
Bsu36I CC'TnA_GG
Cuts at: 0 358 1155
Size: 358 797
BtgI C'CryG_G
Cuts at: 0 746 1155
Size: 746 409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 693 915 1046 1155
Size: 693 222 131 109
Fragments arranged by size:
693 222 131 109
BtsI GCAGTG_nn'
Cuts at: 0 1051 1155
Size: 1051 104
ClaI AT'CG_AT
Cuts at: 0 584 1155
Size: 584 571
DrdI GACnn_nn'nnGTC
Cuts at: 0 781 1155
Size: 781 374
EarI CTCTTCn'nnn_
Cuts at: 0 167 284 475 1155
Size: 167 117 191 680
Fragments arranged by size:
680 191 167 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1134 1155
Size: 1134 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 171 781 904 966 1078 1155
Size: 76 95 610 123 62 112 77
Fragments arranged by size:
610 123 112 95 77 76 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 357 1155
Size: 357 798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 504 1155
Size: 504 651
HaeII r_GCGC'y
Cuts at: 0 16 1155
Size: 16 1139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 716 748 754 786 948 980
Size: 523 32 161 32 6 32 162 32
Cuts at: 980 1113 1145 1155
Size: 133 32 10
Fragments arranged by size:
523 162 161 133 32 32 32 32
32 10 6
KpnI G_GTAC'C
Cuts at: 0 397 1155
Size: 397 758
MslI CAynn'nnrTG
Cuts at: 0 259 425 805 1155
Size: 259 166 380 350
Fragments arranged by size:
380 350 259 166
MspA1I CmG'CkG
Cuts at: 0 54 520 690 954 1155
Size: 54 466 170 264 201
Fragments arranged by size:
466 264 201 170 54
NspI r_CATG'y
Cuts at: 0 849 1155
Size: 849 306
PciI A'CATG_T
Cuts at: 0 845 1155
Size: 845 310
PfoI T'CCnGG_A
Cuts at: 0 758 1155
Size: 758 397
PvuII CAG'CTG
Cuts at: 0 54 520 954 1155
Size: 54 466 434 201
Fragments arranged by size:
466 434 201 54
TatI w'GTAC_w
Cuts at: 0 128 317 1155
Size: 128 189 838
Fragments arranged by size:
838 189 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1155
Size: 1030 32 93
Fragments arranged by size:
1030 93 32
XmnI GAAnn'nnTTC
Cuts at: 0 293 816 1155
Size: 293 523 339
Fragments arranged by size:
523 339 293
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI AvaI BanI BanII
BbsI BcgI BciVI BglI BlpI Bme1580I BpmI BsaBI
BsaHI BsaWI BsaXI BseYI BsiHKAI BsmBI Bsp1286I BspEI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DrdI EarI EciI Eco57MI EcoNI FalI
HaeII Hin4I KpnI MslI MspA1I NspI PciI PfoI
PvuII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BclI BfrBI BglII
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BseRI BsgI BsiEI BsiWI BsmI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BstBI BstEII BstZ17I CspCI DraI
DraIII EaeI EagI EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI