Restriction map for
pF1KB9550
(Linear) (Six-base) MAPSORT of: KIBB9550.seq Check: 1834 from: 1 to: 951
KIBB9550.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AccI GT'mk_AC
Cuts at: 0 713 951
Size: 713 238
AclI AA'CG_TT
Cuts at: 0 764 951
Size: 764 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 951
Size: 349 602
AflIII A'CryG_T
Cuts at: 0 491 951
Size: 491 460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 491 951
Size: 491 460
ApoI r'AATT_y
Cuts at: 0 48 951
Size: 48 903
BanI G'GyrC_C
Cuts at: 0 690 951
Size: 690 261
BanII G_rGCy'C
Cuts at: 0 212 323 452 514 951
Size: 212 111 129 62 437
Fragments arranged by size:
437 212 129 111 62
BbvCI CC'TCA_GC
Cuts at: 0 824 951
Size: 824 127
BclI T'GATC_A
Cuts at: 0 873 951
Size: 873 78
BglI GCCn_nnn'nGGC
Cuts at: 0 279 951
Size: 279 672
BmgBI CAC'GTC
Cuts at: 0 492 555 951
Size: 492 63 396
Fragments arranged by size:
492 396 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 552 951
Size: 552 399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 337 911 951
Size: 178 159 574 40
Fragments arranged by size:
574 178 159 40
Bpu10I CC'TnA_GC
Cuts at: 0 824 951
Size: 824 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 166 906 951
Size: 26 140 740 45
Fragments arranged by size:
740 140 45 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 255 951
Size: 255 696
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 909 939 951
Size: 909 30 12
Fragments arranged by size:
909 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 841 951
Size: 403 438 110
Fragments arranged by size:
438 403 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 951
Size: 77 874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 822 951
Size: 822 129
Bsp1286I G_dGCh'C
Cuts at: 0 212 323 452 514 951
Size: 212 111 129 62 437
Fragments arranged by size:
437 212 129 111 62
BstAPI GCAn_nnn'nTGC
Cuts at: 0 176 951
Size: 176 775
BstYI r'GATC_y
Cuts at: 0 222 617 951
Size: 222 395 334
Fragments arranged by size:
395 334 222
Bsu36I CC'TnA_GG
Cuts at: 0 430 951
Size: 430 521
BtgI C'CryG_G
Cuts at: 0 213 245 951
Size: 213 32 706
Fragments arranged by size:
706 213 32
BtsI GCAGTG_nn'
Cuts at: 0 86 603 832 951
Size: 86 517 229 119
Fragments arranged by size:
517 229 119 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 618 653 951
Size: 618 35 298
Fragments arranged by size:
618 298 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 583 951
Size: 583 368
EaeI y'GGCC_r
Cuts at: 0 531 951
Size: 531 420
EarI CTCTTCn'nnn_
Cuts at: 0 103 951
Size: 103 848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 337 349 911 951
Size: 178 159 12 562 40
Fragments arranged by size:
562 178 159 40 12
EcoO109I rG'GnC_Cy
Cuts at: 0 576 951
Size: 576 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 879 911 951
Size: 158 32 689 32 40
Fragments arranged by size:
689 158 40 32 32
MspA1I CmG'CkG
Cuts at: 0 116 624 696 951
Size: 116 508 72 255
Fragments arranged by size:
508 255 116 72
NcoI C'CATG_G
Cuts at: 0 213 245 951
Size: 213 32 706
Fragments arranged by size:
706 213 32
NspI r_CATG'y
Cuts at: 0 759 951
Size: 759 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 868 951
Size: 868 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 584 616 951
Size: 584 32 335
Fragments arranged by size:
584 335 32
PpuMI rG'GwC_Cy
Cuts at: 0 576 951
Size: 576 375
PstI C_TGCA'G
Cuts at: 0 203 592 951
Size: 203 389 359
Fragments arranged by size:
389 359 203
PvuII CAG'CTG
Cuts at: 0 116 624 951
Size: 116 508 327
Fragments arranged by size:
508 327 116
SfcI C'TryA_G
Cuts at: 0 199 340 588 951
Size: 199 141 248 363
Fragments arranged by size:
363 248 199 141
SmlI C'TyrA_G
Cuts at: 0 41 181 885 951
Size: 41 140 704 66
Fragments arranged by size:
704 140 66 41
SphI G_CATG'C
Cuts at: 0 759 951
Size: 759 192
StyI C'CwwG_G
Cuts at: 0 213 245 352 383 951
Size: 213 32 107 31 568
Fragments arranged by size:
568 213 107 32 31
TatI w'GTAC_w
Cuts at: 0 64 951
Size: 64 887
XmnI GAAnn'nnTTC
Cuts at: 0 599 951
Size: 599 352
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI ApoI BanI BanII
BbvCI BclI BglI BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BsgI BsmBI Bsp1286I BstAPI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI Eco57MI
EcoO109I Hin4I MspA1I NcoI NspI PflMI PpiI PpuMI
PstI PvuII SfcI SmlI SphI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmtI BplI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI ClaI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI ZraI