Restriction map for
pF1KB9545
(Linear) (Six-base) MAPSORT of: KIBB9545.seq Check: 676 from: 1 to: 1629
KIBB9545.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1039 1629
Size: 1039 590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 581 596 1513 1629
Size: 398 183 15 917 116
Fragments arranged by size:
917 398 183 116 15
AflIII A'CryG_T
Cuts at: 0 108 1340 1629
Size: 108 1232 289
Fragments arranged by size:
1232 289 108
AhdI GACnn_n'nnGTC
Cuts at: 0 1442 1629
Size: 1442 187
AleI CACnn'nnGTG
Cuts at: 0 1380 1629
Size: 1380 249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1574 1606 1629
Size: 1574 32 23
Fragments arranged by size:
1574 32 23
ApaLI G'TGCA_C
Cuts at: 0 1373 1629
Size: 1373 256
ApoI r'AATT_y
Cuts at: 0 153 591 988 1290 1316 1610 1629
Size: 153 438 397 302 26 294 19
Fragments arranged by size:
438 397 302 294 153 26 19
AvaI C'yCGr_G
Cuts at: 0 195 1629
Size: 195 1434
BanII G_rGCy'C
Cuts at: 0 89 1112 1629
Size: 89 1023 517
Fragments arranged by size:
1023 517 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 910 1629
Size: 910 719
BbvCI CC'TCA_GC
Cuts at: 0 928 1629
Size: 928 701
BclI T'GATC_A
Cuts at: 0 225 1629
Size: 225 1404
BlpI GC'TnA_GC
Cuts at: 0 83 125 1629
Size: 83 42 1504
Fragments arranged by size:
1504 83 42
Bme1580I G_kGCm'C
Cuts at: 0 1377 1629
Size: 1377 252
BmrI ACTGGGnnnn_n'
Cuts at: 0 1371 1434 1629
Size: 1371 63 195
Fragments arranged by size:
1371 195 63
Bpu10I CC'TnA_GC
Cuts at: 0 928 1466 1629
Size: 928 538 163
Fragments arranged by size:
928 538 163
BsaI GGTCTCn'nnnn_
Cuts at: 0 268 1629
Size: 268 1361
BsaAI yAC'GTr
Cuts at: 0 972 1629
Size: 972 657
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1574 1604 1629
Size: 1574 30 25
Fragments arranged by size:
1574 30 25
BseYI C'CCAG_C
Cuts at: 0 1013 1629
Size: 1013 616
BsiHKAI G_wGCw'C
Cuts at: 0 89 699 1377 1433 1570 1629
Size: 89 610 678 56 137 59
Fragments arranged by size:
678 610 137 89 59 56
Bsp1286I G_dGCh'C
Cuts at: 0 89 699 1112 1377 1433 1570 1629
Size: 89 610 413 265 56 137 59
Fragments arranged by size:
610 413 265 137 89 59 56
BspHI T'CATG_A
Cuts at: 0 270 1629
Size: 270 1359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1039 1629
Size: 1039 590
BsrDI GCAATG_nn'
Cuts at: 0 451 1629
Size: 451 1178
BsrFI r'CCGG_y
Cuts at: 0 1046 1394 1629
Size: 1046 348 235
Fragments arranged by size:
1046 348 235
BssSI C'ACGA_G
Cuts at: 0 506 1629
Size: 506 1123
BstBI TT'CG_AA
Cuts at: 0 533 1629
Size: 533 1096
BtsI GCAGTG_nn'
Cuts at: 0 299 1038 1097 1629
Size: 299 739 59 532
Fragments arranged by size:
739 532 299 59
DraI TTT'AAA
Cuts at: 0 921 1629
Size: 921 708
EarI CTCTTCn'nnn_
Cuts at: 0 14 417 1532 1629
Size: 14 403 1115 97
Fragments arranged by size:
1115 403 97 14
EcoICRI GAG'CTC
Cuts at: 0 87 1629
Size: 87 1542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 581 596 1513 1629
Size: 398 183 15 917 116
Fragments arranged by size:
917 398 183 116 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1158 1629
Size: 1158 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 807 839 1223 1255 1428 1460
Size: 346 32 429 32 384 32 173 32
Cuts at: 1460 1537 1569 1629
Size: 77 32 60
Fragments arranged by size:
429 384 346 173 77 60 32 32
32 32 32
MslI CAynn'nnrTG
Cuts at: 0 113 1341 1380 1561 1629
Size: 113 1228 39 181 68
Fragments arranged by size:
1228 181 113 68 39
MspA1I CmG'CkG
Cuts at: 0 932 1013 1629
Size: 932 81 616
Fragments arranged by size:
932 616 81
NaeI GCC'GGC
Cuts at: 0 1396 1629
Size: 1396 233
NgoMIV G'CCGG_C
Cuts at: 0 1394 1629
Size: 1394 235
NspI r_CATG'y
Cuts at: 0 112 1344 1629
Size: 112 1232 285
Fragments arranged by size:
1232 285 112
PciI A'CATG_T
Cuts at: 0 108 1340 1629
Size: 108 1232 289
Fragments arranged by size:
1232 289 108
PmlI CAC'GTG
Cuts at: 0 972 1629
Size: 972 657
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1574 1606 1629
Size: 1574 32 23
Fragments arranged by size:
1574 32 23
PpuMI rG'GwC_Cy
Cuts at: 0 1158 1629
Size: 1158 471
PvuII CAG'CTG
Cuts at: 0 932 1013 1629
Size: 932 81 616
Fragments arranged by size:
932 616 81
SacI G_AGCT'C
Cuts at: 0 89 1629
Size: 89 1540
SfcI C'TryA_G
Cuts at: 0 1072 1399 1629
Size: 1072 327 230
Fragments arranged by size:
1072 327 230
SmaI CCC'GGG
Cuts at: 0 197 1629
Size: 197 1432
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1227 1629
Size: 1227 402
TatI w'GTAC_w
Cuts at: 0 469 646 1629
Size: 469 177 983
Fragments arranged by size:
983 469 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 1629
Size: 1369 32 228
Fragments arranged by size:
1369 228 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 688 1189 1629
Size: 688 501 440
Fragments arranged by size:
688 501 440
XmaI C'CCGG_G
Cuts at: 0 195 1629
Size: 195 1434
XmnI GAAnn'nnTTC
Cuts at: 0 384 594 1526 1629
Size: 384 210 932 103
Fragments arranged by size:
932 384 210 103
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AloI ApaLI ApoI
AvaI BanII BbsI BbvCI BclI BlpI Bme1580I BmrI
Bpu10I BsaI BsaAI BsaXI BseYI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstBI BtsI DraI EarI
EcoICRI Eco57MI EcoO109I Hin4I MslI MspA1I NaeI NgoMIV
NspI PciI PmlI PpiI PpuMI PvuII SacI SfcI
SmaI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BfrBI BglI BglII BmgBI
BmtI BplI BpmI BpuEI BsaBI BsaHI BsaWI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XhoI ZraI