(Linear) (Six-base) MAPSORT of: KIBB9544.seq  Check: 1785  from: 1  to: 1803



KIBB9544.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AccI GT'mk_AC

Cuts at:      0     313    1803 
   Size:        313    1490
 
AclI AA'CG_TT

Cuts at:      0     102    1803 
   Size:        102    1701
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812    1567    1803 
   Size:        812     755     236

  Fragments arranged by size: 

                 812     755     236
 
AflIII A'CryG_T

Cuts at:      0    1229    1803 
   Size:       1229     574
 
AleI CACnn'nnGTG

Cuts at:      0    1420    1803 
   Size:       1420     383
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1386    1803 
   Size:       1386     417
 
ApoI r'AATT_y

Cuts at:      0     156    1803 
   Size:        156    1647
 
AvaI C'yCGr_G

Cuts at:      0     683    1803 
   Size:        683    1120
 
AvrII C'CTAG_G

Cuts at:      0     708    1803 
   Size:        708    1095
 
BanII G_rGCy'C

Cuts at:      0    1562    1803 
   Size:       1562     241
 
BbvCI CC'TCA_GC

Cuts at:      0    1325    1803 
   Size:       1325     478
 
BclI T'GATC_A

Cuts at:      0      45    1803 
   Size:         45    1758
 
BglI GCCn_nnn'nGGC

Cuts at:      0     794    1803 
   Size:        794    1009
 
Bme1580I G_kGCm'C

Cuts at:      0    1695    1803 
   Size:       1695     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     398     810    1803 
   Size:        398     412     993

  Fragments arranged by size: 

                 993     412     398
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     417    1803 
   Size:        417    1386
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1127    1803 
   Size:       1127     676
 
Bpu10I CC'TnA_GC

Cuts at:      0     146    1325    1603    1803 
   Size:        146    1179     278     200

  Fragments arranged by size: 

                1179     278     200     146
 
BsaBI GATnn'nnATC

Cuts at:      0      44    1803 
   Size:         44    1759
 
BsaHI Gr'CG_yC

Cuts at:      0     809    1803 
   Size:        809     994
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     225     637    1076    1220    1803 
   Size:        225     412     439     144     583

  Fragments arranged by size: 

                 583     439     412     225     144
 
BseYI C'CCAG_C

Cuts at:      0     910    1090    1803 
   Size:        910     180     713

  Fragments arranged by size: 

                 910     713     180
 
BsiHKAI G_wGCw'C

Cuts at:      0     440    1803 
   Size:        440    1363
 
Bsp1286I G_dGCh'C

Cuts at:      0     440    1562    1695    1803 
   Size:        440    1122     133     108

  Fragments arranged by size: 

                1122     440     133     108
 
BsrDI GCAATG_nn'

Cuts at:      0    1778    1803 
   Size:       1778      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1150    1803 
   Size:       1150     653
 
BtsI GCAGTG_nn'

Cuts at:      0     471    1803 
   Size:        471    1332
 
DraI TTT'AAA

Cuts at:      0     569    1803 
   Size:        569    1234
 
EaeI y'GGCC_r

Cuts at:      0      78    1803 
   Size:         78    1725
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     217     414     964    1175    1476    1803 
   Size:        122      95     197     550     211     301     327

  Fragments arranged by size: 

                 550     327     301     211     197     122      95
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812    1127    1567    1803 
   Size:        812     315     440     236

  Fragments arranged by size: 

                 812     440     315     236
 
EcoRI G'AATT_C

Cuts at:      0     156    1803 
   Size:        156    1647
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     282     478     510     610     642     874     906 
   Size:        250      32     196      32     100      32     232      32

Cuts at:    906    1803 
   Size:        897

  Fragments arranged by size: 

                 897     250     232     196     100      32      32      32
                  32
 
MslI CAynn'nnrTG

Cuts at:      0    1148    1420    1516    1803 
   Size:       1148     272      96     287

  Fragments arranged by size: 

                1148     287     272      96
 
MspA1I CmG'CkG

Cuts at:      0      76    1587    1803 
   Size:         76    1511     216

  Fragments arranged by size: 

                1511     216      76
 
NspI r_CATG'y

Cuts at:      0    1233    1712    1803 
   Size:       1233     479      91

  Fragments arranged by size: 

                1233     479      91
 
PciI A'CATG_T

Cuts at:      0    1229    1803 
   Size:       1229     574
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1105    1135    1803 
   Size:       1105      30     668

  Fragments arranged by size: 

                1105     668      30
 
PstI C_TGCA'G

Cuts at:      0    1391    1628    1803 
   Size:       1391     237     175

  Fragments arranged by size: 

                1391     237     175
 
PvuII CAG'CTG

Cuts at:      0      76    1587    1803 
   Size:         76    1511     216

  Fragments arranged by size: 

                1511     216      76
 
SapI GCTCTTCn'nnn_

Cuts at:      0     414     964    1803 
   Size:        414     550     839

  Fragments arranged by size: 

                 839     550     414
 
ScaI AGT'ACT

Cuts at:      0    1484    1803 
   Size:       1484     319
 
SexAI A'CCwGG_T

Cuts at:      0    1241    1803 
   Size:       1241     562
 
SfcI C'TryA_G

Cuts at:      0    1387    1475    1624    1803 
   Size:       1387      88     149     179

  Fragments arranged by size: 

                1387     179     149      88
 
StyI C'CwwG_G

Cuts at:      0     708    1695    1703    1803 
   Size:        708     987       8     100

  Fragments arranged by size: 

                 987     708     100       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1524    1803 
   Size:       1524     279
 
TatI w'GTAC_w

Cuts at:      0    1482    1803 
   Size:       1482     321
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     745     777    1006    1038    1803 
   Size:        745      32     229      32     765

  Fragments arranged by size: 

                 765     745     229      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1619    1803 
   Size:       1619     184
 
XmnI GAAnn'nnTTC

Cuts at:      0     866    1803 
   Size:        866     937
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI
    AvrII    BanII    BbvCI     BclI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BsaBI    BsaHI    BseRI    BseYI  BsiHKAI Bsp1286I
    BsrDI    BstXI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoRI
    Hin4I     MslI   MspA1I     NspI     PciI    PflMI     PstI    PvuII
     SapI     ScaI    SexAI     SfcI     StyI    TaqII     TatI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI
     XmaI     ZraI