(Linear) (Six-base) MAPSORT of: KIBB9538.seq  Check: 4276  from: 1  to: 1056



KIBB9538.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1056 
   Size:        286     770
 
AflII C'TTAA_G

Cuts at:      0      52    1056 
   Size:         52    1004
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1021    1056 
   Size:       1021      35
 
ApoI r'AATT_y

Cuts at:      0     241     530     698    1056 
   Size:        241     289     168     358

  Fragments arranged by size: 

                 358     289     241     168
 
BbvCI CC'TCA_GC

Cuts at:      0     328    1056 
   Size:        328     728
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     548    1056 
   Size:        548     508
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     103     947    1056 
   Size:        103     844     109

  Fragments arranged by size: 

                 844     109     103
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476    1056 
   Size:        476     580
 
Bpu10I CC'TnA_GC

Cuts at:      0     328    1056 
   Size:        328     728
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       2    1056 
   Size:          2    1054
 
BsaWI w'CCGG_w

Cuts at:      0     561    1056 
   Size:        561     495
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     418    1056 
   Size:        418     638
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     663     831    1056 
   Size:        663     168     225

  Fragments arranged by size: 

                 663     225     168
 
BsiHKAI G_wGCw'C

Cuts at:      0     618    1056 
   Size:        618     438
 
BsmI GAATG_Cn'

Cuts at:      0     478    1056 
   Size:        478     578
 
Bsp1286I G_dGCh'C

Cuts at:      0     618    1056 
   Size:        618     438
 
BspEI T'CCGG_A

Cuts at:      0     561    1056 
   Size:        561     495
 
BsrDI GCAATG_nn'

Cuts at:      0     483    1056 
   Size:        483     573
 
BsrGI T'GTAC_A

Cuts at:      0     603    1056 
   Size:        603     453
 
BtsI GCAGTG_nn'

Cuts at:      0     651     819    1056 
   Size:        651     168     237

  Fragments arranged by size: 

                 651     237     168
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     297     332    1056 
   Size:        297      35     724

  Fragments arranged by size: 

                 724     297      35
 
DraI TTT'AAA

Cuts at:      0     351    1056 
   Size:        351     705
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     476    1056 
   Size:        286     190     580

  Fragments arranged by size: 

                 580     286     190
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1002    1056 
   Size:       1002      54
 
EcoRI G'AATT_C

Cuts at:      0     530     698    1056 
   Size:        530     168     358

  Fragments arranged by size: 

                 530     358     168
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     510    1056 
   Size:        510     546
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1007    1025    1039    1056 
   Size:       1007      18      14      17

  Fragments arranged by size: 

                1007      18      17      14
 
HincII GTy'rAC

Cuts at:      0     929    1056 
   Size:        929     127
 
HindIII A'AGCT_T

Cuts at:      0     575    1056 
   Size:        575     481
 
MfeI C'AATT_G

Cuts at:      0      68    1056 
   Size:         68     988
 
MslI CAynn'nnrTG

Cuts at:      0     471     859     975    1056 
   Size:        471     388     116      81

  Fragments arranged by size: 

                 471     388     116      81
 
NdeI CA'TA_TG

Cuts at:      0     468     620     972    1056 
   Size:        468     152     352      84

  Fragments arranged by size: 

                 468     352     152      84
 
PsiI TTA'TAA

Cuts at:      0     146    1056 
   Size:        146     910
 
SmlI C'TyrA_G

Cuts at:      0      52    1056 
   Size:         52    1004
 
SspI AAT'ATT

Cuts at:      0     298    1056 
   Size:        298     758
 
StuI AGG'CCT

Cuts at:      0     997    1056 
   Size:        997      59
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     598    1056 
   Size:        598     458
 
TatI w'GTAC_w

Cuts at:      0     391     603     895    1056 
   Size:        391     212     292     161

  Fragments arranged by size: 

                 391     292     212     161
 
XmnI GAAnn'nnTTC

Cuts at:      0     493     550    1056 
   Size:        493      57     506

  Fragments arranged by size: 

                 506     493      57
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     ApoI    BbvCI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaWI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspEI    BsrDI    BsrGI     BtsI    CspCI     DraI  Eco57MI    EcoNI
    EcoRI     FalI    Hin4I   HincII  HindIII     MfeI     MslI     NdeI
     PsiI     SmlI     SspI     StuI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI