Restriction map for
pF1KB9537
(Linear) (Six-base) MAPSORT of: KIBB9537.seq Check: 2952 from: 1 to: 1185
KIBB9537.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 188 659 1185
Size: 188 471 526
Fragments arranged by size:
526 471 188
AccI GT'mk_AC
Cuts at: 0 464 1185
Size: 464 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1185
Size: 504 681
AflIII A'CryG_T
Cuts at: 0 809 1185
Size: 809 376
AleI CACnn'nnGTG
Cuts at: 0 1063 1185
Size: 1063 122
AlwNI CAG_nnn'CTG
Cuts at: 0 329 731 1003 1152 1185
Size: 329 402 272 149 33
Fragments arranged by size:
402 329 272 149 33
ApaLI G'TGCA_C
Cuts at: 0 745 1185
Size: 745 440
AvaI C'yCGr_G
Cuts at: 0 12 921 1185
Size: 12 909 264
Fragments arranged by size:
909 264 12
BanI G'GyrC_C
Cuts at: 0 43 734 887 1185
Size: 43 691 153 298
Fragments arranged by size:
691 298 153 43
BbeI G_GCGC'C
Cuts at: 0 47 1185
Size: 47 1138
BbvCI CC'TCA_GC
Cuts at: 0 1004 1185
Size: 1004 181
BglI GCCn_nnn'nGGC
Cuts at: 0 885 1185
Size: 885 300
Bme1580I G_kGCm'C
Cuts at: 0 725 749 1185
Size: 725 24 436
Fragments arranged by size:
725 436 24
BmgBI CAC'GTC
Cuts at: 0 201 621 1185
Size: 201 420 564
Fragments arranged by size:
564 420 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 719 792 1185
Size: 719 73 393
Fragments arranged by size:
719 393 73
Bpu10I CC'TnA_GC
Cuts at: 0 1004 1185
Size: 1004 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1185
Size: 940 245
BsaHI Gr'CG_yC
Cuts at: 0 44 151 1185
Size: 44 107 1034
Fragments arranged by size:
1034 107 44
BsaWI w'CCGG_w
Cuts at: 0 369 1185
Size: 369 816
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 188 218 610 640 751 781 1185
Size: 188 30 392 30 111 30 404
Fragments arranged by size:
404 392 188 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 306 550 865 1185
Size: 306 244 315 320
Fragments arranged by size:
320 315 306 244
BseYI C'CCAG_C
Cuts at: 0 125 143 630 1015 1185
Size: 125 18 487 385 170
Fragments arranged by size:
487 385 170 125 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 378 637 865 1185
Size: 26 352 259 228 320
Fragments arranged by size:
352 320 259 228 26
BsiHKAI G_wGCw'C
Cuts at: 0 260 749 1185
Size: 260 489 436
Fragments arranged by size:
489 436 260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 36 191 1185
Size: 36 155 994
Fragments arranged by size:
994 155 36
Bsp1286I G_dGCh'C
Cuts at: 0 260 725 749 1185
Size: 260 465 24 436
Fragments arranged by size:
465 436 260 24
BspHI T'CATG_A
Cuts at: 0 908 1185
Size: 908 277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 188 617 659 1185
Size: 98 90 429 42 526
Fragments arranged by size:
526 429 98 90 42
BsrBI CCG'CTC
Cuts at: 0 515 1185
Size: 515 670
BsrFI r'CCGG_y
Cuts at: 0 134 1185
Size: 134 1051
BstBI TT'CG_AA
Cuts at: 0 1028 1185
Size: 1028 157
BstXI CCAn_nnnn'nTGG
Cuts at: 0 673 1185
Size: 673 512
Bsu36I CC'TnA_GG
Cuts at: 0 988 1185
Size: 988 197
BtgI C'CryG_G
Cuts at: 0 155 600 658 1185
Size: 155 445 58 527
Fragments arranged by size:
527 445 155 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 948 1185
Size: 948 237
BtsI GCAGTG_nn'
Cuts at: 0 894 1185
Size: 894 291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 522 557 1185
Size: 522 35 628
Fragments arranged by size:
628 522 35
DraIII CAC_nnn'GTG
Cuts at: 0 744 1185
Size: 744 441
EaeI y'GGCC_r
Cuts at: 0 803 1185
Size: 803 382
EarI CTCTTCn'nnn_
Cuts at: 0 568 575 932 967 1185
Size: 568 7 357 35 218
Fragments arranged by size:
568 357 218 35 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1185
Size: 504 681
EcoNI CCTnn'n_nnAGG
Cuts at: 0 321 991 1185
Size: 321 670 194
Fragments arranged by size:
670 321 194
EcoO109I rG'GnC_Cy
Cuts at: 0 472 585 914 1185
Size: 472 113 329 271
Fragments arranged by size:
472 329 271 113
FspI TGC'GCA
Cuts at: 0 1085 1185
Size: 1085 100
HaeII r_GCGC'y
Cuts at: 0 47 113 144 819 1185
Size: 47 66 31 675 366
Fragments arranged by size:
675 366 66 47 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 610 642 751 783 1185
Size: 263 32 315 32 109 32 402
Fragments arranged by size:
402 315 263 109 32 32 32
KasI G'GCGC_C
Cuts at: 0 43 1185
Size: 43 1142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1185
Size: 434 751
MscI TGG'CCA
Cuts at: 0 805 1185
Size: 805 380
MslI CAynn'nnrTG
Cuts at: 0 1063 1185
Size: 1063 122
MspA1I CmG'CkG
Cuts at: 0 157 485 524 1003 1152 1185
Size: 157 328 39 479 149 33
Fragments arranged by size:
479 328 157 149 39 33
NaeI GCC'GGC
Cuts at: 0 136 1185
Size: 136 1049
NarI GG'CG_CC
Cuts at: 0 44 1185
Size: 44 1141
NgoMIV G'CCGG_C
Cuts at: 0 134 1185
Size: 134 1051
NspI r_CATG'y
Cuts at: 0 813 1185
Size: 813 372
PasI CC'CwG_GG
Cuts at: 0 323 571 1185
Size: 323 248 614
Fragments arranged by size:
614 323 248
PciI A'CATG_T
Cuts at: 0 809 1185
Size: 809 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 731 812 1104 1185
Size: 731 81 292 81
Fragments arranged by size:
731 292 81 81
PfoI T'CCnGG_A
Cuts at: 0 230 1185
Size: 230 955
PpuMI rG'GwC_Cy
Cuts at: 0 914 1185
Size: 914 271
PstI C_TGCA'G
Cuts at: 0 1148 1185
Size: 1148 37
PvuII CAG'CTG
Cuts at: 0 485 524 1152 1185
Size: 485 39 628 33
Fragments arranged by size:
628 485 39 33
SacII CC_GC'GG
Cuts at: 0 158 1185
Size: 158 1027
SexAI A'CCwGG_T
Cuts at: 0 740 1185
Size: 740 445
SfcI C'TryA_G
Cuts at: 0 1144 1185
Size: 1144 41
SfoI GGC'GCC
Cuts at: 0 45 1185
Size: 45 1140
SmaI CCC'GGG
Cuts at: 0 14 1185
Size: 14 1171
SmlI C'TyrA_G
Cuts at: 0 955 1185
Size: 955 230
SrfI GCCC'GGGC
Cuts at: 0 14 1185
Size: 14 1171
StyI C'CwwG_G
Cuts at: 0 683 1076 1185
Size: 683 393 109
Fragments arranged by size:
683 393 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 671 1185
Size: 135 536 514
Fragments arranged by size:
536 514 135
TatI w'GTAC_w
Cuts at: 0 296 398 1185
Size: 296 102 787
Fragments arranged by size:
787 296 102
XmaI C'CCGG_G
Cuts at: 0 12 1185
Size: 12 1173
XmnI GAAnn'nnTTC
Cuts at: 0 424 1185
Size: 424 761
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaLI AvaI
BanI BbeI BbvCI BglI Bme1580I BmgBI BmrI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstBI BstXI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PstI PvuII SacII SexAI
SfcI SfoI SmaI SmlI SrfI StyI TaqII TatI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI BpmI BsaI BsaAI BsaBI BsiEI
BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstEII BstYI BstZ17I ClaI DraI DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI ZraI