Restriction map for
pF1KB9536
(Linear) (Six-base) MAPSORT of: KIBB9536.seq Check: 2859 from: 1 to: 1206
KIBB9536.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1206
Size: 474 732
AfeI AGC'GCT
Cuts at: 0 338 1206
Size: 338 868
AflIII A'CryG_T
Cuts at: 0 382 1206
Size: 382 824
AleI CACnn'nnGTG
Cuts at: 0 533 785 963 1121 1206
Size: 533 252 178 158 85
Fragments arranged by size:
533 252 178 158 85
ApaLI G'TGCA_C
Cuts at: 0 766 1206
Size: 766 440
ApoI r'AATT_y
Cuts at: 0 373 402 938 1206
Size: 373 29 536 268
Fragments arranged by size:
536 373 268 29
AvaI C'yCGr_G
Cuts at: 0 556 1206
Size: 556 650
AvrII C'CTAG_G
Cuts at: 0 993 1206
Size: 993 213
BamHI G'GATC_C
Cuts at: 0 272 1206
Size: 272 934
BanI G'GyrC_C
Cuts at: 0 724 851 1206
Size: 724 127 355
Fragments arranged by size:
724 355 127
BfrBI ATG'CAT
Cuts at: 0 96 1206
Size: 96 1110
BglI GCCn_nnn'nGGC
Cuts at: 0 449 1206
Size: 449 757
BglII A'GATC_T
Cuts at: 0 252 588 918 1206
Size: 252 336 330 288
Fragments arranged by size:
336 330 288 252
BlpI GC'TnA_GC
Cuts at: 0 1168 1206
Size: 1168 38
Bme1580I G_kGCm'C
Cuts at: 0 770 1206
Size: 770 436
BmrI ACTGGGnnnn_n'
Cuts at: 0 206 626 710 1206
Size: 206 420 84 496
Fragments arranged by size:
496 420 206 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 1206
Size: 1183 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1054 1206
Size: 1054 152
BsiHKAI G_wGCw'C
Cuts at: 0 770 1206
Size: 770 436
BsmI GAATG_Cn'
Cuts at: 0 145 1206
Size: 145 1061
Bsp1286I G_dGCh'C
Cuts at: 0 770 1206
Size: 770 436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 355 1206
Size: 355 851
BsrGI T'GTAC_A
Cuts at: 0 2 681 927 1206
Size: 2 679 246 279
Fragments arranged by size:
679 279 246 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 965 1206
Size: 965 241
BstYI r'GATC_y
Cuts at: 0 252 272 588 918 1206
Size: 252 20 316 330 288
Fragments arranged by size:
330 316 288 252 20
DraI TTT'AAA
Cuts at: 0 879 1206
Size: 879 327
EaeI y'GGCC_r
Cuts at: 0 744 1206
Size: 744 462
EarI CTCTTCn'nnn_
Cuts at: 0 8 310 944 1206
Size: 8 302 634 262
Fragments arranged by size:
634 302 262 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 474 1206
Size: 474 732
EcoO109I rG'GnC_Cy
Cuts at: 0 626 1206
Size: 626 580
EcoRI G'AATT_C
Cuts at: 0 938 1206
Size: 938 268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 1206
Size: 168 1038
HaeII r_GCGC'y
Cuts at: 0 340 1206
Size: 340 866
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 405 437 1206
Size: 102 32 271 32 769
Fragments arranged by size:
769 271 102 32 32
HindIII A'AGCT_T
Cuts at: 0 569 900 1074 1206
Size: 569 331 174 132
Fragments arranged by size:
569 331 174 132
MscI TGG'CCA
Cuts at: 0 746 1206
Size: 746 460
MslI CAynn'nnrTG
Cuts at: 0 95 465 533 549 785 963 1121 1206
Size: 95 370 68 16 236 178 158 85
Fragments arranged by size:
370 236 178 158 95 85 68 16
NdeI CA'TA_TG
Cuts at: 0 92 1206
Size: 92 1114
NsiI A_TGCA'T
Cuts at: 0 98 1206
Size: 98 1108
NspI r_CATG'y
Cuts at: 0 364 386 1206
Size: 364 22 820
Fragments arranged by size:
820 364 22
PciI A'CATG_T
Cuts at: 0 382 1206
Size: 382 824
PsiI TTA'TAA
Cuts at: 0 212 296 1206
Size: 212 84 910
Fragments arranged by size:
910 212 84
SapI GCTCTTCn'nnn_
Cuts at: 0 310 1206
Size: 310 896
SfcI C'TryA_G
Cuts at: 0 909 1206
Size: 909 297
SmlI C'TyrA_G
Cuts at: 0 556 1162 1206
Size: 556 606 44
Fragments arranged by size:
606 556 44
StyI C'CwwG_G
Cuts at: 0 993 1206
Size: 993 213
TatI w'GTAC_w
Cuts at: 0 2 499 681 927 1113 1206
Size: 2 497 182 246 186 93
Fragments arranged by size:
497 246 186 182 93 2
XhoI C'TCGA_G
Cuts at: 0 556 1206
Size: 556 650
Enzymes that do cut:
AcuI AfeI AflIII AleI ApaLI ApoI AvaI AvrII
BamHI BanI BfrBI BglI BglII BlpI Bme1580I BmrI
BpuEI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstXI
BstYI DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HindIII MscI MslI NdeI NsiI NspI
PciI PsiI SapI SfcI SmlI StyI TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI AlwNI ApaI AscI AseI AsiSI BaeI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI XmnI ZraI