Restriction map for
pF1KB9522
(Linear) (Six-base) MAPSORT of: KIBB9522.seq Check: 5367 from: 1 to: 2061
KIBB9522.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1453 2061
Size: 1453 608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 861 1698 2017 2061
Size: 589 272 837 319 44
Fragments arranged by size:
837 589 319 272 44
AflIII A'CryG_T
Cuts at: 0 524 1803 2061
Size: 524 1279 258
Fragments arranged by size:
1279 524 258
AhdI GACnn_n'nnGTC
Cuts at: 0 675 2061
Size: 675 1386
AleI CACnn'nnGTG
Cuts at: 0 1756 2061
Size: 1756 305
AlwNI CAG_nnn'CTG
Cuts at: 0 15 567 633 744 1393 1770 2061
Size: 15 552 66 111 649 377 291
Fragments arranged by size:
649 552 377 291 111 66 15
ApaI G_GGCC'C
Cuts at: 0 397 2061
Size: 397 1664
ApaLI G'TGCA_C
Cuts at: 0 1192 1315 2061
Size: 1192 123 746
Fragments arranged by size:
1192 746 123
ApoI r'AATT_y
Cuts at: 0 906 2061
Size: 906 1155
AvaI C'yCGr_G
Cuts at: 0 1071 1295 1465 1976 2034 2061
Size: 1071 224 170 511 58 27
Fragments arranged by size:
1071 511 224 170 58 27
BamHI G'GATC_C
Cuts at: 0 1670 2061
Size: 1670 391
BanI G'GyrC_C
Cuts at: 0 64 1507 1879 2061
Size: 64 1443 372 182
Fragments arranged by size:
1443 372 182 64
BanII G_rGCy'C
Cuts at: 0 397 436 536 983 2061
Size: 397 39 100 447 1078
Fragments arranged by size:
1078 447 397 100 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 95 1058 1332 2061
Size: 95 963 274 729
Fragments arranged by size:
963 729 274 95
BbvCI CC'TCA_GC
Cuts at: 0 1825 2061
Size: 1825 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 153 187 1344 1378 2061
Size: 153 34 1157 34 683
Fragments arranged by size:
1157 683 153 34 34
BlpI GC'TnA_GC
Cuts at: 0 556 1366 1537 2061
Size: 556 810 171 524
Fragments arranged by size:
810 556 524 171
Bme1580I G_kGCm'C
Cuts at: 0 69 397 1196 1319 2061
Size: 69 328 799 123 742
Fragments arranged by size:
799 742 328 123 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 449 2061
Size: 3 446 1612
Fragments arranged by size:
1612 446 3
BmtI G_CTAG'C
Cuts at: 0 1750 2061
Size: 1750 311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 404 1310 2061
Size: 404 906 751
Fragments arranged by size:
906 751 404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 655 703 721 1266 1622 2061
Size: 532 123 48 18 545 356 439
Fragments arranged by size:
545 532 439 356 123 48 18
Bpu10I CC'TnA_GC
Cuts at: 0 389 1207 1825 2061
Size: 389 818 618 236
Fragments arranged by size:
818 618 389 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1516 2061
Size: 573 943 545
Fragments arranged by size:
943 573 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 1093 1173 2061
Size: 1093 80 888
Fragments arranged by size:
1093 888 80
BsaAI yAC'GTr
Cuts at: 0 1224 2061
Size: 1224 837
BsaHI Gr'CG_yC
Cuts at: 0 1187 1646 2061
Size: 1187 459 415
Fragments arranged by size:
1187 459 415
BsaWI w'CCGG_w
Cuts at: 0 692 2061
Size: 692 1369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 1264 1294 2061
Size: 739 30 495 30 767
Fragments arranged by size:
767 739 495 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 391 700 781 784 853 1161 1204
Size: 346 45 309 81 3 69 308 43
Cuts at: 1204 2061
Size: 857
Fragments arranged by size:
857 346 309 308 81 69 45 43
3
BseYI C'CCAG_C
Cuts at: 0 292 1090 1550 1818 2061
Size: 292 798 460 268 243
Fragments arranged by size:
798 460 292 268 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1411 1783 2061
Size: 730 681 372 278
Fragments arranged by size:
730 681 372 278
BsiEI CG_ry'CG
Cuts at: 0 503 2061
Size: 503 1558
BsiHKAI G_wGCw'C
Cuts at: 0 536 992 1196 1319 2061
Size: 536 456 204 123 742
Fragments arranged by size:
742 536 456 204 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1242 2061
Size: 1242 819
Bsp1286I G_dGCh'C
Cuts at: 0 69 397 436 536 983 992 1196 1319
Size: 69 328 39 100 447 9 204 123
Cuts at: 1319 2061
Size: 742
Fragments arranged by size:
742 447 328 204 123 100 69 39
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 752 1334 1453 2061
Size: 167 585 582 119 608
Fragments arranged by size:
608 585 582 167 119
BsrBI CCG'CTC
Cuts at: 0 505 2061
Size: 505 1556
BsrDI GCAATG_nn'
Cuts at: 0 1039 1262 2061
Size: 1039 223 799
Fragments arranged by size:
1039 799 223
BsrFI r'CCGG_y
Cuts at: 0 730 1374 2061
Size: 730 644 687
Fragments arranged by size:
730 687 644
BssSI C'ACGA_G
Cuts at: 0 1353 1489 2061
Size: 1353 136 572
Fragments arranged by size:
1353 572 136
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1770 2061
Size: 1770 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1569 1620 1758 2061
Size: 1569 51 138 303
Fragments arranged by size:
1569 303 138 51
BstYI r'GATC_y
Cuts at: 0 1670 1766 2061
Size: 1670 96 295
Fragments arranged by size:
1670 295 96
BtgI C'CryG_G
Cuts at: 0 69 704 983 1544 1754 2061
Size: 69 635 279 561 210 307
Fragments arranged by size:
635 561 307 279 210 69
BtsI GCAGTG_nn'
Cuts at: 0 1092 1163 1813 2061
Size: 1092 71 650 248
Fragments arranged by size:
1092 650 248 71
DraIII CAC_nnn'GTG
Cuts at: 0 710 2061
Size: 710 1351
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1684 2061
Size: 9 1675 377
Fragments arranged by size:
1675 377 9
EaeI y'GGCC_r
Cuts at: 0 500 1334 1739 2061
Size: 500 834 405 322
Fragments arranged by size:
834 500 405 322
EagI C'GGCC_G
Cuts at: 0 500 2061
Size: 500 1561
EarI CTCTTCn'nnn_
Cuts at: 0 55 179 823 850 1717 1993 2061
Size: 55 124 644 27 867 276 68
Fragments arranged by size:
867 644 276 124 68 55 27
EcoICRI GAG'CTC
Cuts at: 0 534 2061
Size: 534 1527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 589 655 703 721 861 1266 1622
Size: 532 57 66 48 18 140 405 356
Cuts at: 1622 1698 2017 2061
Size: 76 319 44
Fragments arranged by size:
532 405 356 319 140 76 66 57
48 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 591 895 2061
Size: 388 203 304 1166
Fragments arranged by size:
1166 388 304 203
EcoO109I rG'GnC_Cy
Cuts at: 0 189 255 393 394 543 596 741 2061
Size: 189 66 138 1 149 53 145 1320
Fragments arranged by size:
1320 189 149 145 138 66 53 1
EcoRI G'AATT_C
Cuts at: 0 906 2061
Size: 906 1155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 342 2061
Size: 342 1719
FspI TGC'GCA
Cuts at: 0 163 2061
Size: 163 1898
HaeII r_GCGC'y
Cuts at: 0 1544 2018 2061
Size: 1544 474 43
Fragments arranged by size:
1544 474 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 331 363 661 693 2061
Size: 288 32 11 32 298 32 1368
Fragments arranged by size:
1368 298 288 32 32 32 11
MscI TGG'CCA
Cuts at: 0 1741 2061
Size: 1741 320
MslI CAynn'nnrTG
Cuts at: 0 1312 1741 1756 1759 2061
Size: 1312 429 15 3 302
Fragments arranged by size:
1312 429 302 15 3
MspA1I CmG'CkG
Cuts at: 0 111 402 552 2061
Size: 111 291 150 1509
Fragments arranged by size:
1509 291 150 111
NaeI GCC'GGC
Cuts at: 0 732 2061
Size: 732 1329
NcoI C'CATG_G
Cuts at: 0 1544 1754 2061
Size: 1544 210 307
Fragments arranged by size:
1544 307 210
NgoMIV G'CCGG_C
Cuts at: 0 730 2061
Size: 730 1331
NheI G'CTAG_C
Cuts at: 0 1746 2061
Size: 1746 315
NspI r_CATG'y
Cuts at: 0 292 528 1807 2061
Size: 292 236 1279 254
Fragments arranged by size:
1279 292 254 236
PasI CC'CwG_GG
Cuts at: 0 251 1847 2061
Size: 251 1596 214
Fragments arranged by size:
1596 251 214
PciI A'CATG_T
Cuts at: 0 524 1803 2061
Size: 524 1279 258
Fragments arranged by size:
1279 524 258
PflMI CCAn_nnn'nTGG
Cuts at: 0 710 921 1550 1667 2061
Size: 710 211 629 117 394
Fragments arranged by size:
710 629 394 211 117
PfoI T'CCnGG_A
Cuts at: 0 737 896 1282 1453 1673 2061
Size: 737 159 386 171 220 388
Fragments arranged by size:
737 388 386 220 171 159
PpuMI rG'GwC_Cy
Cuts at: 0 543 741 2061
Size: 543 198 1320
Fragments arranged by size:
1320 543 198
PspOMI G'GGCC_C
Cuts at: 0 393 2061
Size: 393 1668
PspXI vC'TCGA_Gb
Cuts at: 0 1465 2061
Size: 1465 596
PstI C_TGCA'G
Cuts at: 0 23 106 219 1973 2061
Size: 23 83 113 1754 88
Fragments arranged by size:
1754 113 88 83 23
PvuII CAG'CTG
Cuts at: 0 552 2061
Size: 552 1509
SacI G_AGCT'C
Cuts at: 0 536 2061
Size: 536 1525
SapI GCTCTTCn'nnn_
Cuts at: 0 55 1993 2061
Size: 55 1938 68
Fragments arranged by size:
1938 68 55
ScaI AGT'ACT
Cuts at: 0 793 2061
Size: 793 1268
SfcI C'TryA_G
Cuts at: 0 19 102 215 1009 1969 2061
Size: 19 83 113 794 960 92
Fragments arranged by size:
960 794 113 92 83 19
SmaI CCC'GGG
Cuts at: 0 1073 1978 2061
Size: 1073 905 83
Fragments arranged by size:
1073 905 83
SmlI C'TyrA_G
Cuts at: 0 588 1465 1531 2061
Size: 588 877 66 530
Fragments arranged by size:
877 588 530 66
StuI AGG'CCT
Cuts at: 0 586 2061
Size: 586 1475
StyI C'CwwG_G
Cuts at: 0 60 1544 1754 1836 1929 2061
Size: 60 1484 210 82 93 132
Fragments arranged by size:
1484 210 132 93 82 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 1082 1804 2061
Size: 913 169 722 257
Fragments arranged by size:
913 722 257 169
TatI w'GTAC_w
Cuts at: 0 791 2061
Size: 791 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 739 771 2061
Size: 214 32 493 32 1290
Fragments arranged by size:
1290 493 214 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 2061
Size: 918 1143
XhoI C'TCGA_G
Cuts at: 0 1465 2061
Size: 1465 596
XmaI C'CCGG_G
Cuts at: 0 1071 1976 2061
Size: 1071 905 85
Fragments arranged by size:
1071 905 85
XmnI GAAnn'nnTTC
Cuts at: 0 92 2061
Size: 92 1969
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SapI ScaI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BclI BfrBI BglI BglII BmgBI BsaBI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI EciI EcoRV FseI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI ZraI