Restriction map for
pF1KB9517
(Linear) (Six-base) MAPSORT of: KIBB9517.seq Check: 6708 from: 1 to: 1695
KIBB9517.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1236 1695
Size: 1236 459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1372 1594 1695
Size: 158 1214 222 101
Fragments arranged by size:
1214 222 158 101
AflIII A'CryG_T
Cuts at: 0 71 755 1695
Size: 71 684 940
Fragments arranged by size:
940 684 71
AgeI A'CCGG_T
Cuts at: 0 977 1695
Size: 977 718
AhdI GACnn_n'nnGTC
Cuts at: 0 1126 1695
Size: 1126 569
AleI CACnn'nnGTG
Cuts at: 0 481 1487 1695
Size: 481 1006 208
Fragments arranged by size:
1006 481 208
AlwNI CAG_nnn'CTG
Cuts at: 0 288 1036 1532 1545 1695
Size: 288 748 496 13 150
Fragments arranged by size:
748 496 288 150 13
ApaI G_GGCC'C
Cuts at: 0 66 1404 1460 1695
Size: 66 1338 56 235
Fragments arranged by size:
1338 235 66 56
ApaLI G'TGCA_C
Cuts at: 0 759 1642 1695
Size: 759 883 53
Fragments arranged by size:
883 759 53
ApoI r'AATT_y
Cuts at: 0 907 1695
Size: 907 788
AvaI C'yCGr_G
Cuts at: 0 221 634 1695
Size: 221 413 1061
Fragments arranged by size:
1061 413 221
BamHI G'GATC_C
Cuts at: 0 936 1695
Size: 936 759
BanI G'GyrC_C
Cuts at: 0 488 542 630 1695
Size: 488 54 88 1065
Fragments arranged by size:
1065 488 88 54
BanII G_rGCy'C
Cuts at: 0 66 146 1404 1460 1695
Size: 66 80 1258 56 235
Fragments arranged by size:
1258 235 80 66 56
BbeI G_GCGC'C
Cuts at: 0 546 1695
Size: 546 1149
BbsI GAAGACnn'nnnn_
Cuts at: 0 1393 1582 1695
Size: 1393 189 113
Fragments arranged by size:
1393 189 113
BclI T'GATC_A
Cuts at: 0 1190 1695
Size: 1190 505
BlpI GC'TnA_GC
Cuts at: 0 835 1695
Size: 835 860
Bme1580I G_kGCm'C
Cuts at: 0 66 635 763 1404 1460 1470 1646 1695
Size: 66 569 128 641 56 10 176 49
Fragments arranged by size:
641 569 176 128 66 56 49 10
BmgBI CAC'GTC
Cuts at: 0 419 1650 1695
Size: 419 1231 45
Fragments arranged by size:
1231 419 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 1695
Size: 425 1270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1695
Size: 130 1565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1309 1695
Size: 1309 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 1695
Size: 103 1592
BsaAI yAC'GTr
Cuts at: 0 808 1583 1695
Size: 808 775 112
Fragments arranged by size:
808 775 112
BsaBI GATnn'nnATC
Cuts at: 0 664 1695
Size: 664 1031
BsaHI Gr'CG_yC
Cuts at: 0 543 1695
Size: 543 1152
BsaWI w'CCGG_w
Cuts at: 0 977 1695
Size: 977 718
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 343 373 1087 1117 1695
Size: 83 30 230 30 714 30 578
Fragments arranged by size:
714 578 230 83 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 170 351 607 738 793 826 1108 1219
Size: 170 181 256 131 55 33 282 111
Cuts at: 1219 1255 1354 1450 1695
Size: 36 99 96 245
Fragments arranged by size:
282 256 245 181 170 131 111 99
96 55 36 33
BseYI C'CCAG_C
Cuts at: 0 104 229 1445 1695
Size: 104 125 1216 250
Fragments arranged by size:
1216 250 125 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1399 1511 1695
Size: 1297 102 112 184
Fragments arranged by size:
1297 184 112 102
BsiEI CG_ry'CG
Cuts at: 0 1139 1695
Size: 1139 556
BsiHKAI G_wGCw'C
Cuts at: 0 308 763 1172 1646 1695
Size: 308 455 409 474 49
Fragments arranged by size:
474 455 409 308 49
BsiWI C'GTAC_G
Cuts at: 0 1253 1695
Size: 1253 442
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 1695
Size: 363 1332
Bsp1286I G_dGCh'C
Cuts at: 0 66 146 308 635 763 1172 1404 1460
Size: 66 80 162 327 128 409 232 56
Cuts at: 1460 1470 1646 1695
Size: 10 176 49
Fragments arranged by size:
409 327 232 176 162 128 80 66
56 49 10
BspHI T'CATG_A
Cuts at: 0 870 1187 1652 1695
Size: 870 317 465 43
Fragments arranged by size:
870 465 317 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 839 1236 1695
Size: 839 397 459
Fragments arranged by size:
839 459 397
BsrDI GCAATG_nn'
Cuts at: 0 1517 1695
Size: 1517 178
BsrFI r'CCGG_y
Cuts at: 0 977 1304 1507 1695
Size: 977 327 203 188
Fragments arranged by size:
977 327 203 188
BsrGI T'GTAC_A
Cuts at: 0 1103 1695
Size: 1103 592
BstAPI GCAn_nnn'nTGC
Cuts at: 0 263 1422 1535 1695
Size: 263 1159 113 160
Fragments arranged by size:
1159 263 160 113
BstYI r'GATC_y
Cuts at: 0 198 936 1695
Size: 198 738 759
Fragments arranged by size:
759 738 198
Bsu36I CC'TnA_GG
Cuts at: 0 496 533 674 1695
Size: 496 37 141 1021
Fragments arranged by size:
1021 496 141 37
BtgI C'CryG_G
Cuts at: 0 395 455 707 1695
Size: 395 60 252 988
Fragments arranged by size:
988 395 252 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1092 1695
Size: 1092 603
BtsI GCAGTG_nn'
Cuts at: 0 87 523 1482 1494 1695
Size: 87 436 959 12 201
Fragments arranged by size:
959 436 201 87 12
DraIII CAC_nnn'GTG
Cuts at: 0 1422 1695
Size: 1422 273
EaeI y'GGCC_r
Cuts at: 0 324 1234 1695
Size: 324 910 461
Fragments arranged by size:
910 461 324
EarI CTCTTCn'nnn_
Cuts at: 0 857 1695
Size: 857 838
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 807 919 1695
Size: 807 112 776
Fragments arranged by size:
807 776 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 158 1372 1594 1695
Size: 130 28 1214 222 101
Fragments arranged by size:
1214 222 130 101 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 1695
Size: 898 797
EcoO109I rG'GnC_Cy
Cuts at: 0 62 63 879 1120 1401 1456 1457 1695
Size: 62 1 816 241 281 55 1 238
Fragments arranged by size:
816 281 241 238 62 55 1 1
EcoRI G'AATT_C
Cuts at: 0 907 1695
Size: 907 788
HaeII r_GCGC'y
Cuts at: 0 230 546 842 1695
Size: 230 316 296 853
Fragments arranged by size:
853 316 296 230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1112 1144 1695
Size: 673 32 407 32 551
Fragments arranged by size:
673 551 407 32 32
KasI G'GCGC_C
Cuts at: 0 542 1695
Size: 542 1153
MscI TGG'CCA
Cuts at: 0 326 1695
Size: 326 1369
MslI CAynn'nnrTG
Cuts at: 0 481 1186 1487 1695
Size: 481 705 301 208
Fragments arranged by size:
705 481 301 208
MspA1I CmG'CkG
Cuts at: 0 317 387 1545 1674 1695
Size: 317 70 1158 129 21
Fragments arranged by size:
1158 317 129 70 21
NarI GG'CG_CC
Cuts at: 0 543 1695
Size: 543 1152
NcoI C'CATG_G
Cuts at: 0 707 1695
Size: 707 988
NspI r_CATG'y
Cuts at: 0 75 759 1625 1695
Size: 75 684 866 70
Fragments arranged by size:
866 684 75 70
PasI CC'CwG_GG
Cuts at: 0 958 1695
Size: 958 737
PciI A'CATG_T
Cuts at: 0 71 755 1695
Size: 71 684 940
Fragments arranged by size:
940 684 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 401 1695
Size: 401 1294
PfoI T'CCnGG_A
Cuts at: 0 146 614 1695
Size: 146 468 1081
Fragments arranged by size:
1081 468 146
PmlI CAC'GTG
Cuts at: 0 808 1583 1695
Size: 808 775 112
Fragments arranged by size:
808 775 112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 714 746 1695
Size: 714 32 949
Fragments arranged by size:
949 714 32
PpuMI rG'GwC_Cy
Cuts at: 0 1120 1695
Size: 1120 575
PspOMI G'GGCC_C
Cuts at: 0 62 1400 1456 1695
Size: 62 1338 56 239
Fragments arranged by size:
1338 239 62 56
PspXI vC'TCGA_Gb
Cuts at: 0 221 1695
Size: 221 1474
PstI C_TGCA'G
Cuts at: 0 82 719 902 1489 1695
Size: 82 637 183 587 206
Fragments arranged by size:
637 587 206 183 82
PvuII CAG'CTG
Cuts at: 0 387 1545 1674 1695
Size: 387 1158 129 21
Fragments arranged by size:
1158 387 129 21
SanDI GG'GwC_CC
Cuts at: 0 1120 1695
Size: 1120 575
SapI GCTCTTCn'nnn_
Cuts at: 0 857 1695
Size: 857 838
SexAI A'CCwGG_T
Cuts at: 0 1418 1695
Size: 1418 277
SfcI C'TryA_G
Cuts at: 0 78 715 898 1485 1695
Size: 78 637 183 587 210
Fragments arranged by size:
637 587 210 183 78
SfoI GGC'GCC
Cuts at: 0 544 1695
Size: 544 1151
SmlI C'TyrA_G
Cuts at: 0 221 1324 1695
Size: 221 1103 371
Fragments arranged by size:
1103 371 221
StuI AGG'CCT
Cuts at: 0 1349 1695
Size: 1349 346
StyI C'CwwG_G
Cuts at: 0 707 1695
Size: 707 988
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 531 1695
Size: 531 1164
TatI w'GTAC_w
Cuts at: 0 1103 1695
Size: 1103 592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 930 1695
Size: 438 492 765
Fragments arranged by size:
765 492 438
XhoI C'TCGA_G
Cuts at: 0 221 1695
Size: 221 1474
XmnI GAAnn'nnTTC
Cuts at: 0 866 1695
Size: 866 829
Enzymes that do cut:
AarI AcuI AflIII AgeI AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BclI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BstAPI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuII SanDI SapI SexAI SfcI
SfoI SmlI StuI StyI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AlfI AloI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BfrBI BglI BglII BmtI BplI Bpu10I BsmI BspEI
BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI DrdI EagI EcoICRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PshAI PsiI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmaI ZraI