Restriction map for
pF1KB9515
(Linear) (Six-base) MAPSORT of: KIBB9515.seq Check: 1265 from: 1 to: 1533
KIBB9515.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 1533
Size: 672 861
AccI GT'mk_AC
Cuts at: 0 34 459 941 1533
Size: 34 425 482 592
Fragments arranged by size:
592 482 425 34
Acc65I G'GTAC_C
Cuts at: 0 860 1533
Size: 860 673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1533
Size: 357 1176
AfeI AGC'GCT
Cuts at: 0 1338 1533
Size: 1338 195
AflIII A'CryG_T
Cuts at: 0 516 1533
Size: 516 1017
AlwNI CAG_nnn'CTG
Cuts at: 0 256 585 1533
Size: 256 329 948
Fragments arranged by size:
948 329 256
ApoI r'AATT_y
Cuts at: 0 228 410 799 1032 1533
Size: 228 182 389 233 501
Fragments arranged by size:
501 389 233 228 182
AseI AT'TA_AT
Cuts at: 0 1406 1533
Size: 1406 127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 360 393 1533
Size: 360 33 1140
Fragments arranged by size:
1140 360 33
BanI G'GyrC_C
Cuts at: 0 860 1533
Size: 860 673
BanII G_rGCy'C
Cuts at: 0 11 840 1533
Size: 11 829 693
Fragments arranged by size:
829 693 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 733 1533
Size: 733 800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1334 1533
Size: 593 741 199
Fragments arranged by size:
741 593 199
BsaHI Gr'CG_yC
Cuts at: 0 1448 1533
Size: 1448 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1444 1474 1533
Size: 1444 30 59
Fragments arranged by size:
1444 59 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1001 1533
Size: 1001 532
BseYI C'CCAG_C
Cuts at: 0 1297 1533
Size: 1297 236
BsiEI CG_ry'CG
Cuts at: 0 41 1533
Size: 41 1492
BsiHKAI G_wGCw'C
Cuts at: 0 1238 1533
Size: 1238 295
Bsp1286I G_dGCh'C
Cuts at: 0 11 840 1238 1533
Size: 11 829 398 295
Fragments arranged by size:
829 398 295 11
BspHI T'CATG_A
Cuts at: 0 450 1533
Size: 450 1083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 672 1533
Size: 672 861
BsrDI GCAATG_nn'
Cuts at: 0 1512 1533
Size: 1512 21
BsrFI r'CCGG_y
Cuts at: 0 80 1533
Size: 80 1453
BsrGI T'GTAC_A
Cuts at: 0 869 1533
Size: 869 664
BssSI C'ACGA_G
Cuts at: 0 122 1533
Size: 122 1411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1165 1329 1533
Size: 1165 164 204
Fragments arranged by size:
1165 204 164
BstYI r'GATC_y
Cuts at: 0 316 1196 1533
Size: 316 880 337
Fragments arranged by size:
880 337 316
BstZ17I GTA'TAC
Cuts at: 0 942 1533
Size: 942 591
DraI TTT'AAA
Cuts at: 0 486 1533
Size: 486 1047
EaeI y'GGCC_r
Cuts at: 0 1217 1533
Size: 1217 316
EarI CTCTTCn'nnn_
Cuts at: 0 164 346 1238 1533
Size: 164 182 892 295
Fragments arranged by size:
892 295 182 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 593 1334 1533
Size: 357 236 741 199
Fragments arranged by size:
741 357 236 199
EcoRI G'AATT_C
Cuts at: 0 799 1533
Size: 799 734
EcoRV GAT'ATC
Cuts at: 0 132 633 687 1533
Size: 132 501 54 846
Fragments arranged by size:
846 501 132 54
HaeII r_GCGC'y
Cuts at: 0 1340 1533
Size: 1340 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 1407 1439 1533
Size: 190 32 1185 32 94
Fragments arranged by size:
1185 190 94 32 32
HincII GTy'rAC
Cuts at: 0 35 360 1533
Size: 35 325 1173
Fragments arranged by size:
1173 325 35
KpnI G_GTAC'C
Cuts at: 0 864 1533
Size: 864 669
MfeI C'AATT_G
Cuts at: 0 17 1533
Size: 17 1516
MluI A'CGCG_T
Cuts at: 0 516 1533
Size: 516 1017
MscI TGG'CCA
Cuts at: 0 1219 1533
Size: 1219 314
MspA1I CmG'CkG
Cuts at: 0 1165 1301 1533
Size: 1165 136 232
Fragments arranged by size:
1165 232 136
NdeI CA'TA_TG
Cuts at: 0 669 1533
Size: 669 864
NspI r_CATG'y
Cuts at: 0 870 1293 1533
Size: 870 423 240
Fragments arranged by size:
870 423 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 1069 1533
Size: 1069 464
PstI C_TGCA'G
Cuts at: 0 960 1170 1533
Size: 960 210 363
Fragments arranged by size:
960 363 210
PvuII CAG'CTG
Cuts at: 0 1165 1533
Size: 1165 368
SalI G'TCGA_C
Cuts at: 0 33 1533
Size: 33 1500
ScaI AGT'ACT
Cuts at: 0 1307 1533
Size: 1307 226
SfcI C'TryA_G
Cuts at: 0 777 956 1166 1533
Size: 777 179 210 367
Fragments arranged by size:
777 367 210 179
SphI G_CATG'C
Cuts at: 0 1293 1533
Size: 1293 240
SspI AAT'ATT
Cuts at: 0 1527 1533
Size: 1527 6
StyI C'CwwG_G
Cuts at: 0 11 1388 1533
Size: 11 1377 145
Fragments arranged by size:
1377 145 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 662 1415 1533
Size: 55 607 753 118
Fragments arranged by size:
753 607 118 55
TatI w'GTAC_w
Cuts at: 0 869 1305 1533
Size: 869 436 228
Fragments arranged by size:
869 436 228
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlwNI ApoI
AseI BaeI BanI BanII BbsI BpmI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI BstZ17I DraI EaeI
EarI Eco57MI EcoRI EcoRV HaeII Hin4I HincII KpnI
MfeI MluI MscI MspA1I NdeI NspI PflMI PstI
PvuII SalI ScaI SfcI SphI SspI StyI TaqII
TatI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BsrBI BssHII BstBI BstEII BstXI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I FalI FseI FspI FspAI HindIII HpaI
KasI MmeI MslI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI