Restriction map for
pF1KB9513
(Linear) (Six-base) MAPSORT of: KIBB9513.seq Check: 2744 from: 1 to: 1440
KIBB9513.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 97 1440
Size: 97 1343
Acc65I G'GTAC_C
Cuts at: 0 299 1440
Size: 299 1141
AflIII A'CryG_T
Cuts at: 0 404 1376 1440
Size: 404 972 64
Fragments arranged by size:
972 404 64
AleI CACnn'nnGTG
Cuts at: 0 352 1440
Size: 352 1088
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1238 1270 1440
Size: 1238 32 170
Fragments arranged by size:
1238 170 32
AlwNI CAG_nnn'CTG
Cuts at: 0 537 759 1440
Size: 537 222 681
Fragments arranged by size:
681 537 222
BamHI G'GATC_C
Cuts at: 0 415 1440
Size: 415 1025
BanI G'GyrC_C
Cuts at: 0 299 698 1042 1440
Size: 299 399 344 398
Fragments arranged by size:
399 398 344 299
BanII G_rGCy'C
Cuts at: 0 164 494 1112 1440
Size: 164 330 618 328
Fragments arranged by size:
618 330 328 164
BbsI GAAGACnn'nnnn_
Cuts at: 0 823 1440
Size: 823 617
BbvCI CC'TCA_GC
Cuts at: 0 64 538 1440
Size: 64 474 902
Fragments arranged by size:
902 474 64
BfrBI ATG'CAT
Cuts at: 0 1420 1440
Size: 1420 20
Bme1580I G_kGCm'C
Cuts at: 0 1047 1342 1440
Size: 1047 295 98
Fragments arranged by size:
1047 295 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 623 1235 1440
Size: 623 612 205
Fragments arranged by size:
623 612 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1440
Size: 832 608
Bpu10I CC'TnA_GC
Cuts at: 0 64 538 1440
Size: 64 474 902
Fragments arranged by size:
902 474 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 270 601 1440
Size: 270 331 839
Fragments arranged by size:
839 331 270
BsaBI GATnn'nnATC
Cuts at: 0 548 1440
Size: 548 892
BsaHI Gr'CG_yC
Cuts at: 0 1274 1440
Size: 1274 166
BsaWI w'CCGG_w
Cuts at: 0 418 1256 1440
Size: 418 838 184
Fragments arranged by size:
838 418 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 551 581 1440
Size: 551 30 859
Fragments arranged by size:
859 551 30
BseYI C'CCAG_C
Cuts at: 0 211 1440
Size: 211 1229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 532 1440
Size: 330 202 908
Fragments arranged by size:
908 330 202
BsiEI CG_ry'CG
Cuts at: 0 1074 1440
Size: 1074 366
BsiHKAI G_wGCw'C
Cuts at: 0 494 959 1112 1440
Size: 494 465 153 328
Fragments arranged by size:
494 465 328 153
Bsp1286I G_dGCh'C
Cuts at: 0 164 494 959 1047 1112 1342 1440
Size: 164 330 465 88 65 230 98
Fragments arranged by size:
465 330 230 164 98 88 65
BspEI T'CCGG_A
Cuts at: 0 418 1256 1440
Size: 418 838 184
Fragments arranged by size:
838 418 184
BspHI T'CATG_A
Cuts at: 0 1131 1440
Size: 1131 309
BstAPI GCAn_nnn'nTGC
Cuts at: 0 519 1440
Size: 519 921
BstBI TT'CG_AA
Cuts at: 0 431 1440
Size: 431 1009
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1440
Size: 843 597
BstYI r'GATC_y
Cuts at: 0 415 1440
Size: 415 1025
BtgI C'CryG_G
Cuts at: 0 288 1440
Size: 288 1152
BtsI GCAGTG_nn'
Cuts at: 0 1404 1440
Size: 1404 36
EaeI y'GGCC_r
Cuts at: 0 218 833 1071 1440
Size: 218 615 238 369
Fragments arranged by size:
615 369 238 218
EagI C'GGCC_G
Cuts at: 0 1071 1440
Size: 1071 369
EarI CTCTTCn'nnn_
Cuts at: 0 1100 1440
Size: 1100 340
EcoICRI GAG'CTC
Cuts at: 0 492 1110 1440
Size: 492 618 330
Fragments arranged by size:
618 492 330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1440
Size: 832 608
EcoRV GAT'ATC
Cuts at: 0 546 1440
Size: 546 894
HincII GTy'rAC
Cuts at: 0 98 948 1440
Size: 98 850 492
Fragments arranged by size:
850 492 98
HindIII A'AGCT_T
Cuts at: 0 389 1440
Size: 389 1051
KpnI G_GTAC'C
Cuts at: 0 303 1440
Size: 303 1137
MscI TGG'CCA
Cuts at: 0 835 1440
Size: 835 605
MslI CAynn'nnrTG
Cuts at: 0 352 1440
Size: 352 1088
MspA1I CmG'CkG
Cuts at: 0 534 810 1440
Size: 534 276 630
Fragments arranged by size:
630 534 276
NsiI A_TGCA'T
Cuts at: 0 1422 1440
Size: 1422 18
NspI r_CATG'y
Cuts at: 0 277 408 1380 1424 1440
Size: 277 131 972 44 16
Fragments arranged by size:
972 277 131 44 16
PasI CC'CwG_GG
Cuts at: 0 1389 1440
Size: 1389 51
PciI A'CATG_T
Cuts at: 0 404 1376 1440
Size: 404 972 64
Fragments arranged by size:
972 404 64
PflMI CCAn_nnn'nTGG
Cuts at: 0 581 759 1440
Size: 581 178 681
Fragments arranged by size:
681 581 178
PstI C_TGCA'G
Cuts at: 0 74 591 1440
Size: 74 517 849
Fragments arranged by size:
849 517 74
PvuII CAG'CTG
Cuts at: 0 534 810 1440
Size: 534 276 630
Fragments arranged by size:
630 534 276
SacI G_AGCT'C
Cuts at: 0 494 1112 1440
Size: 494 618 328
Fragments arranged by size:
618 494 328
SalI G'TCGA_C
Cuts at: 0 96 1440
Size: 96 1344
SapI GCTCTTCn'nnn_
Cuts at: 0 1100 1440
Size: 1100 340
SfcI C'TryA_G
Cuts at: 0 70 587 1440
Size: 70 517 853
Fragments arranged by size:
853 517 70
StyI C'CwwG_G
Cuts at: 0 1163 1189 1440
Size: 1163 26 251
Fragments arranged by size:
1163 251 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 485 1030 1381 1440
Size: 392 93 545 351 59
Fragments arranged by size:
545 392 351 93 59
TatI w'GTAC_w
Cuts at: 0 197 1019 1440
Size: 197 822 421
Fragments arranged by size:
822 421 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1068 1100 1440
Size: 1068 32 340
Fragments arranged by size:
1068 340 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 674 1440
Size: 674 766
Enzymes that do cut:
AccI Acc65I AflIII AleI AloI AlwNI BamHI BanI
BanII BbsI BbvCI BfrBI Bme1580I BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BstAPI BstBI BstXI BstYI
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoRV
HincII HindIII KpnI MscI MslI MspA1I NsiI NspI
PasI PciI PflMI PstI PvuII SacI SalI SapI
SfcI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BseRI BsiWI
BsmI BsmBI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI