Restriction map for
pF1KB9512
(Linear) (Six-base) MAPSORT of: KIBB9512.seq Check: 3268 from: 1 to: 1509
KIBB9512.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 93 511 820 994 1509
Size: 93 418 309 174 515
Fragments arranged by size:
515 418 309 174 93
Acc65I G'GTAC_C
Cuts at: 0 919 1509
Size: 919 590
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1509
Size: 1057 452
AflIII A'CryG_T
Cuts at: 0 872 1509
Size: 872 637
AlwNI CAG_nnn'CTG
Cuts at: 0 415 1509
Size: 415 1094
ApaI G_GGCC'C
Cuts at: 0 194 463 1509
Size: 194 269 1046
Fragments arranged by size:
1046 269 194
ApaLI G'TGCA_C
Cuts at: 0 1134 1509
Size: 1134 375
AseI AT'TA_AT
Cuts at: 0 232 1509
Size: 232 1277
AvaI C'yCGr_G
Cuts at: 0 31 224 263 1234 1500 1509
Size: 31 193 39 971 266 9
Fragments arranged by size:
971 266 193 39 31 9
BanI G'GyrC_C
Cuts at: 0 349 919 1509
Size: 349 570 590
Fragments arranged by size:
590 570 349
BanII G_rGCy'C
Cuts at: 0 8 194 385 463 569 1069 1207 1235
Size: 8 186 191 78 106 500 138 28
Cuts at: 1235 1337 1509
Size: 102 172
Fragments arranged by size:
500 191 186 172 138 106 102 78
28 8
BbvCI CC'TCA_GC
Cuts at: 0 482 1509
Size: 482 1027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1225 1259 1509
Size: 1225 34 250
Fragments arranged by size:
1225 250 34
BglI GCCn_nnn'nGGC
Cuts at: 0 16 1509
Size: 16 1493
Bme1580I G_kGCm'C
Cuts at: 0 194 463 1138 1509
Size: 194 269 675 371
Fragments arranged by size:
675 371 269 194
BmgBI CAC'GTC
Cuts at: 0 693 1509
Size: 693 816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1509
Size: 1083 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 425 1509
Size: 161 264 1084
Fragments arranged by size:
1084 264 161
Bpu10I CC'TnA_GC
Cuts at: 0 482 1463 1509
Size: 482 981 46
Fragments arranged by size:
981 482 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1509
Size: 653 856
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 1047 1509
Size: 733 314 462
Fragments arranged by size:
733 462 314
BsaWI w'CCGG_w
Cuts at: 0 371 1433 1509
Size: 371 1062 76
Fragments arranged by size:
1062 371 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 593 623 1509
Size: 385 30 178 30 886
Fragments arranged by size:
886 385 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 109 224 1509
Size: 106 3 115 1285
Fragments arranged by size:
1285 115 106 3
BseYI C'CCAG_C
Cuts at: 0 336 1509
Size: 336 1173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 147 1509
Size: 142 5 1362
Fragments arranged by size:
1362 142 5
BsiEI CG_ry'CG
Cuts at: 0 27 1509
Size: 27 1482
BsiHKAI G_wGCw'C
Cuts at: 0 569 1138 1509
Size: 569 569 371
Fragments arranged by size:
569 569 371
BsmI GAATG_Cn'
Cuts at: 0 1248 1361 1509
Size: 1248 113 148
Fragments arranged by size:
1248 148 113
Bsp1286I G_dGCh'C
Cuts at: 0 8 194 385 463 569 1069 1138 1207
Size: 8 186 191 78 106 500 69 69
Cuts at: 1207 1235 1337 1509
Size: 28 102 172
Fragments arranged by size:
500 191 186 172 106 102 78 69
69 28 8
BspEI T'CCGG_A
Cuts at: 0 371 1509
Size: 371 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 511 820 994 1304 1509
Size: 93 418 309 174 310 205
Fragments arranged by size:
418 310 309 205 174 93
BsrFI r'CCGG_y
Cuts at: 0 10 346 1449 1509
Size: 10 336 1103 60
Fragments arranged by size:
1103 336 60 10
BstYI r'GATC_y
Cuts at: 0 1093 1509
Size: 1093 416
BtgI C'CryG_G
Cuts at: 0 449 513 1509
Size: 449 64 996
Fragments arranged by size:
996 449 64
BtsI GCAGTG_nn'
Cuts at: 0 159 1509
Size: 159 1350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 743 778 1509
Size: 743 35 731
Fragments arranged by size:
743 731 35
DraIII CAC_nnn'GTG
Cuts at: 0 492 1509
Size: 492 1017
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1509
Size: 1039 470
EaeI y'GGCC_r
Cuts at: 0 24 149 1509
Size: 24 125 1360
Fragments arranged by size:
1360 125 24
EagI C'GGCC_G
Cuts at: 0 24 1509
Size: 24 1485
EarI CTCTTCn'nnn_
Cuts at: 0 719 1509
Size: 719 790
EcoICRI GAG'CTC
Cuts at: 0 567 1509
Size: 567 942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 425 1057 1509
Size: 161 264 632 452
Fragments arranged by size:
632 452 264 161
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1300 1509
Size: 1300 209
EcoO109I rG'GnC_Cy
Cuts at: 0 190 460 708 1497 1509
Size: 190 270 248 789 12
Fragments arranged by size:
789 270 248 190 12
EcoRV GAT'ATC
Cuts at: 0 1275 1509
Size: 1275 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 595 627 649 681 1509
Size: 385 32 178 32 22 32 828
Fragments arranged by size:
828 385 178 32 32 32 22
KpnI G_GTAC'C
Cuts at: 0 923 1509
Size: 923 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1367 1509
Size: 839 528 142
Fragments arranged by size:
839 528 142
MslI CAynn'nnrTG
Cuts at: 0 968 1509
Size: 968 541
MspA1I CmG'CkG
Cuts at: 0 39 139 849 1015 1081 1509
Size: 39 100 710 166 66 428
Fragments arranged by size:
710 428 166 100 66 39
NaeI GCC'GGC
Cuts at: 0 12 348 1451 1509
Size: 12 336 1103 58
Fragments arranged by size:
1103 336 58 12
NdeI CA'TA_TG
Cuts at: 0 1281 1509
Size: 1281 228
NgoMIV G'CCGG_C
Cuts at: 0 10 346 1449 1509
Size: 10 336 1103 60
Fragments arranged by size:
1103 336 60 10
PasI CC'CwG_GG
Cuts at: 0 1200 1509
Size: 1200 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 200 1509
Size: 200 1309
PfoI T'CCnGG_A
Cuts at: 0 877 1509
Size: 877 632
PpuMI rG'GwC_Cy
Cuts at: 0 708 1497 1509
Size: 708 789 12
Fragments arranged by size:
789 708 12
PspOMI G'GGCC_C
Cuts at: 0 190 459 1509
Size: 190 269 1050
Fragments arranged by size:
1050 269 190
PstI C_TGCA'G
Cuts at: 0 104 1509
Size: 104 1405
PvuII CAG'CTG
Cuts at: 0 139 849 1509
Size: 139 710 660
Fragments arranged by size:
710 660 139
SacI G_AGCT'C
Cuts at: 0 569 1509
Size: 569 940
SanDI GG'GwC_CC
Cuts at: 0 708 1509
Size: 708 801
SexAI A'CCwGG_T
Cuts at: 0 797 853 1509
Size: 797 56 656
Fragments arranged by size:
797 656 56
SfcI C'TryA_G
Cuts at: 0 100 615 844 1509
Size: 100 515 229 665
Fragments arranged by size:
665 515 229 100
SmaI CCC'GGG
Cuts at: 0 226 265 1509
Size: 226 39 1244
Fragments arranged by size:
1244 226 39
SmlI C'TyrA_G
Cuts at: 0 668 1509
Size: 668 841
StyI C'CwwG_G
Cuts at: 0 635 761 1301 1509
Size: 635 126 540 208
Fragments arranged by size:
635 540 208 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 771 1114 1509
Size: 540 231 343 395
Fragments arranged by size:
540 395 343 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 593 625 1509
Size: 593 32 884
Fragments arranged by size:
884 593 32
Tth111I GACn'n_nGTC
Cuts at: 0 1420 1509
Size: 1420 89
XmaI C'CCGG_G
Cuts at: 0 224 263 1509
Size: 224 39 1246
Fragments arranged by size:
1246 224 39
XmnI GAAnn'nnTTC
Cuts at: 0 547 1509
Size: 547 962
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaI ApaLI AseI
AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrFI BstYI BtgI BtsI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
KpnI MmeI MslI MspA1I NaeI NdeI NgoMIV PasI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SexAI SfcI SmaI SmlI StyI TaqII TstI Tth111I
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BsaAI BsaBI BsaHI BsiWI BsmBI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DraI EciI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MscI NarI NcoI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XcmI
XhoI ZraI