Restriction map for
pF1KB9511
(Linear) (Six-base) MAPSORT of: KIBB9511.seq Check: 9669 from: 1 to: 1158
KIBB9511.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 206 1158
Size: 206 952
Acc65I G'GTAC_C
Cuts at: 0 510 1158
Size: 510 648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1100 1158
Size: 389 711 58
Fragments arranged by size:
711 389 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1130 1158
Size: 1130 28
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 1158
Size: 886 32 240
Fragments arranged by size:
886 240 32
ApaI G_GGCC'C
Cuts at: 0 103 1158
Size: 103 1055
ApoI r'AATT_y
Cuts at: 0 585 1158
Size: 585 573
BanI G'GyrC_C
Cuts at: 0 510 1158
Size: 510 648
BanII G_rGCy'C
Cuts at: 0 103 1158
Size: 103 1055
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 9 43 1158
Size: 9 34 1115
Fragments arranged by size:
1115 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 186 1158
Size: 186 972
BglII A'GATC_T
Cuts at: 0 372 984 1158
Size: 372 612 174
Fragments arranged by size:
612 372 174
Bme1580I G_kGCm'C
Cuts at: 0 103 1158
Size: 103 1055
BmrI ACTGGGnnnn_n'
Cuts at: 0 900 1029 1158
Size: 900 129 129
Fragments arranged by size:
900 129 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 691 879 1158
Size: 643 48 188 279
Fragments arranged by size:
643 279 188 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 826 1158
Size: 826 332
BsaBI GATnn'nnATC
Cuts at: 0 983 1158
Size: 983 175
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 431 1158
Size: 266 165 727
Fragments arranged by size:
727 266 165
BsiEI CG_ry'CG
Cuts at: 0 33 1158
Size: 33 1125
BsmI GAATG_Cn'
Cuts at: 0 633 1158
Size: 633 525
Bsp1286I G_dGCh'C
Cuts at: 0 103 1158
Size: 103 1055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1014 1158
Size: 1014 144
BsrBI CCG'CTC
Cuts at: 0 80 1158
Size: 80 1078
BsrDI GCAATG_nn'
Cuts at: 0 414 1158
Size: 414 744
BstBI TT'CG_AA
Cuts at: 0 663 1158
Size: 663 495
BstXI CCAn_nnnn'nTGG
Cuts at: 0 857 1158
Size: 857 301
BstYI r'GATC_y
Cuts at: 0 330 372 984 1158
Size: 330 42 612 174
Fragments arranged by size:
612 330 174 42
BtgI C'CryG_G
Cuts at: 0 155 1158
Size: 155 1003
EaeI y'GGCC_r
Cuts at: 0 30 654 696 996 1041 1158
Size: 30 624 42 300 45 117
Fragments arranged by size:
624 300 117 45 42 30
EagI C'GGCC_G
Cuts at: 0 30 1158
Size: 30 1128
EarI CTCTTCn'nnn_
Cuts at: 0 643 1158
Size: 643 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 643 691 879 1100 1158
Size: 389 254 48 188 221 58
Fragments arranged by size:
389 254 221 188 58 48
EcoO109I rG'GnC_Cy
Cuts at: 0 100 432 1026 1158
Size: 100 332 594 132
Fragments arranged by size:
594 332 132 100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 135 1158
Size: 135 1023
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 1158
Size: 651 32 475
Fragments arranged by size:
651 475 32
KpnI G_GTAC'C
Cuts at: 0 514 1158
Size: 514 644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1158
Size: 527 631
MscI TGG'CCA
Cuts at: 0 656 698 998 1043 1158
Size: 656 42 300 45 115
Fragments arranged by size:
656 300 115 45 42
MslI CAynn'nnrTG
Cuts at: 0 1058 1158
Size: 1058 100
MspA1I CmG'CkG
Cuts at: 0 65 1158
Size: 65 1093
NcoI C'CATG_G
Cuts at: 0 155 1158
Size: 155 1003
NspI r_CATG'y
Cuts at: 0 24 582 1158
Size: 24 558 576
Fragments arranged by size:
576 558 24
PpuMI rG'GwC_Cy
Cuts at: 0 432 1026 1158
Size: 432 594 132
Fragments arranged by size:
594 432 132
PspOMI G'GGCC_C
Cuts at: 0 99 1158
Size: 99 1059
PstI C_TGCA'G
Cuts at: 0 384 1158
Size: 384 774
SexAI A'CCwGG_T
Cuts at: 0 506 1158
Size: 506 652
SfcI C'TryA_G
Cuts at: 0 380 746 1158
Size: 380 366 412
Fragments arranged by size:
412 380 366
SphI G_CATG'C
Cuts at: 0 24 582 1158
Size: 24 558 576
Fragments arranged by size:
576 558 24
StyI C'CwwG_G
Cuts at: 0 133 155 1158
Size: 133 22 1003
Fragments arranged by size:
1003 133 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 460 1158
Size: 460 698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 1158
Size: 93 1065
Enzymes that do cut:
AccI Acc65I AcuI AlfI AloI ApaI ApoI BanI
BanII BcgI BciVI BglII Bme1580I BmrI BpmI BsaI
BsaBI BseRI BsiEI BsmI Bsp1286I BspMI BsrBI BsrDI
BstBI BstXI BstYI BtgI EaeI EagI EarI Eco57MI
EcoO109I FalI Hin4I KpnI MmeI MscI MslI MspA1I
NcoI NspI PpuMI PspOMI PstI SexAI SfcI SphI
StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI