Restriction map for
pF1KB9509
(Linear) (Six-base) MAPSORT of: KIBB9509.seq Check: 2289 from: 1 to: 1080
KIBB9509.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 163 310 652 970 1080
Size: 92 71 147 342 318 110
Fragments arranged by size:
342 318 147 110 92 71
AflII C'TTAA_G
Cuts at: 0 543 1080
Size: 543 537
AleI CACnn'nnGTG
Cuts at: 0 107 1080
Size: 107 973
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1076 1080
Size: 1076 4
AlwNI CAG_nnn'CTG
Cuts at: 0 141 675 750 1080
Size: 141 534 75 330
Fragments arranged by size:
534 330 141 75
ApoI r'AATT_y
Cuts at: 0 321 1080
Size: 321 759
BbsI GAAGACnn'nnnn_
Cuts at: 0 307 355 1080
Size: 307 48 725
Fragments arranged by size:
725 307 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 96 130 1080
Size: 96 34 950
Fragments arranged by size:
950 96 34
BfrBI ATG'CAT
Cuts at: 0 335 1080
Size: 335 745
BglI GCCn_nnn'nGGC
Cuts at: 0 785 1080
Size: 785 295
BmrI ACTGGGnnnn_n'
Cuts at: 0 1005 1080
Size: 1005 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 433 451 649 829 951 988 1003
Size: 367 66 18 198 180 122 37 15
Cuts at: 1003 1080
Size: 77
Fragments arranged by size:
367 198 180 122 77 66 37 18
15
Bpu10I CC'TnA_GC
Cuts at: 0 775 1080
Size: 775 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1080
Size: 943 137
BsaBI GATnn'nnATC
Cuts at: 0 248 1080
Size: 248 832
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 622 652 961 991 1080
Size: 622 30 309 30 89
Fragments arranged by size:
622 309 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 181 364 1080
Size: 169 12 183 716
Fragments arranged by size:
716 183 169 12
BseYI C'CCAG_C
Cuts at: 0 576 1080
Size: 576 504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 1080
Size: 767 313
BsrBI CCG'CTC
Cuts at: 0 159 987 1080
Size: 159 828 93
Fragments arranged by size:
828 159 93
BsrDI GCAATG_nn'
Cuts at: 0 839 1080
Size: 839 241
BsrFI r'CCGG_y
Cuts at: 0 719 1016 1080
Size: 719 297 64
Fragments arranged by size:
719 297 64
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 966 1080
Size: 219 747 114
Fragments arranged by size:
747 219 114
BstYI r'GATC_y
Cuts at: 0 375 580 1080
Size: 375 205 500
Fragments arranged by size:
500 375 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 98 1080
Size: 98 982
BtsI GCAGTG_nn'
Cuts at: 0 114 1080
Size: 114 966
ClaI AT'CG_AT
Cuts at: 0 1058 1080
Size: 1058 22
DrdI GACnn_nn'nnGTC
Cuts at: 0 61 1080
Size: 61 1019
EaeI y'GGCC_r
Cuts at: 0 119 1080
Size: 119 961
EarI CTCTTCn'nnn_
Cuts at: 0 511 1080
Size: 511 569
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 304 1035 1080
Size: 22 282 731 45
Fragments arranged by size:
731 282 45 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 163 310 367 433 451 649 652
Size: 92 71 147 57 66 18 198 3
Cuts at: 652 829 951 970 988 1003 1080
Size: 177 122 19 18 15 77
Fragments arranged by size:
198 177 147 122 92 77 71 66
57 19 18 18 15 3
EcoO109I rG'GnC_Cy
Cuts at: 0 171 804 1005 1080
Size: 171 633 201 75
Fragments arranged by size:
633 201 171 75
HaeII r_GCGC'y
Cuts at: 0 421 1080
Size: 421 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 565 597 723 755 829 861
Size: 393 32 140 32 126 32 74 32
Cuts at: 861 1080
Size: 219
Fragments arranged by size:
393 219 140 126 74 32 32 32
32
HincII GTy'rAC
Cuts at: 0 54 480 488 516 1080
Size: 54 426 8 28 564
Fragments arranged by size:
564 426 54 28 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1080
Size: 815 265
MslI CAynn'nnrTG
Cuts at: 0 107 1080
Size: 107 973
NsiI A_TGCA'T
Cuts at: 0 337 1080
Size: 337 743
PacI TTA_AT'TAA
Cuts at: 0 329 1080
Size: 329 751
PpuMI rG'GwC_Cy
Cuts at: 0 171 1005 1080
Size: 171 834 75
Fragments arranged by size:
834 171 75
PstI C_TGCA'G
Cuts at: 0 746 1080
Size: 746 334
SexAI A'CCwGG_T
Cuts at: 0 749 1080
Size: 749 331
SfcI C'TryA_G
Cuts at: 0 355 562 742 1080
Size: 355 207 180 338
Fragments arranged by size:
355 338 207 180
SmlI C'TyrA_G
Cuts at: 0 543 922 1080
Size: 543 379 158
Fragments arranged by size:
543 379 158
XbaI T'CTAG_A
Cuts at: 0 438 1080
Size: 438 642
Enzymes that do cut:
AcuI AflII AleI AlfI AlwNI ApoI BbsI BcgI
BfrBI BglI BmrI BpmI Bpu10I BpuEI BsaBI BsaXI
BseRI BseYI BspMI BsrBI BsrDI BsrFI BstXI BstYI
BtgZI BtsI ClaI DrdI EaeI EarI EciI Eco57MI
EcoO109I HaeII Hin4I HincII MmeI MslI NsiI PacI
PpuMI PstI SexAI SfcI SmlI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BciVI
BclI BglII BlpI Bme1580I BmgBI BmtI BplI BsaI
BsaAI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI CspCI DraI DraIII
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI