Restriction map for
pF1KB9504
(Linear) (Six-base) MAPSORT of: KIBB9504.seq Check: 7619 from: 1 to: 3942
KIBB9504.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1841 3226 3942
Size: 1841 1385 716
Fragments arranged by size:
1841 1385 716
AccI GT'mk_AC
Cuts at: 0 2486 3942
Size: 2486 1456
Acc65I G'GTAC_C
Cuts at: 0 2939 3942
Size: 2939 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1543 1725 3942
Size: 1543 182 2217
Fragments arranged by size:
2217 1543 182
AfeI AGC'GCT
Cuts at: 0 3625 3942
Size: 3625 317
AflIII A'CryG_T
Cuts at: 0 2786 3942
Size: 2786 1156
AleI CACnn'nnGTG
Cuts at: 0 385 3271 3517 3883 3942
Size: 385 2886 246 366 59
Fragments arranged by size:
2886 385 366 246 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 1276 1618 3484 3552 3619 3659 3942
Size: 547 729 342 1866 68 67 40 283
Fragments arranged by size:
1866 729 547 342 283 68 67 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1496 1528 3942
Size: 1496 32 2414
Fragments arranged by size:
2414 1496 32
AlwNI CAG_nnn'CTG
Cuts at: 0 61 189 1083 1521 1812 1974 2065 2171
Size: 61 128 894 438 291 162 91 106
Cuts at: 2171 2295 2775 3354 3477 3592 3613 3690 3727
Size: 124 480 579 123 115 21 77 37
Cuts at: 3727 3843 3942
Size: 116 99
Fragments arranged by size:
894 579 480 438 291 162 128 124
123 116 115 106 99 91 77 61
37 21
ApaI G_GGCC'C
Cuts at: 0 2892 3942
Size: 2892 1050
ApaLI G'TGCA_C
Cuts at: 0 453 1072 2650 3942
Size: 453 619 1578 1292
Fragments arranged by size:
1578 1292 619 453
ApoI r'AATT_y
Cuts at: 0 3127 3942
Size: 3127 815
AvaI C'yCGr_G
Cuts at: 0 2203 2587 2827 3942
Size: 2203 384 240 1115
Fragments arranged by size:
2203 1115 384 240
BamHI G'GATC_C
Cuts at: 0 2903 3942
Size: 2903 1039
BanI G'GyrC_C
Cuts at: 0 364 2375 2865 2939 3075 3273 3942
Size: 364 2011 490 74 136 198 669
Fragments arranged by size:
2011 669 490 364 198 136 74
BanII G_rGCy'C
Cuts at: 0 437 907 1321 1355 1898 2669 2892 3584
Size: 437 470 414 34 543 771 223 692
Cuts at: 3584 3942
Size: 358
Fragments arranged by size:
771 692 543 470 437 414 358 223
34
BbeI G_GCGC'C
Cuts at: 0 2869 3942
Size: 2869 1073
BbsI GAAGACnn'nnnn_
Cuts at: 0 1293 1764 1846 3942
Size: 1293 471 82 2096
Fragments arranged by size:
2096 1293 471 82
BbvCI CC'TCA_GC
Cuts at: 0 2053 2278 3933 3942
Size: 2053 225 1655 9
Fragments arranged by size:
2053 1655 225 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2539 2573 3942
Size: 2539 34 1369
Fragments arranged by size:
2539 1369 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1817 2013 3942
Size: 1817 196 1929
Fragments arranged by size:
1929 1817 196
BlpI GC'TnA_GC
Cuts at: 0 1264 2128 3164 3942
Size: 1264 864 1036 778
Fragments arranged by size:
1264 1036 864 778
Bme1580I G_kGCm'C
Cuts at: 0 457 658 825 1048 1076 2380 2654 2746
Size: 457 201 167 223 28 1304 274 92
Cuts at: 2746 2892 3942
Size: 146 1050
Fragments arranged by size:
1304 1050 457 274 223 201 167 146
92 28
BmgBI CAC'GTC
Cuts at: 0 1284 3942
Size: 1284 2658
BmrI ACTGGGnnnn_n'
Cuts at: 0 661 763 3123 3909 3942
Size: 661 102 2360 786 33
Fragments arranged by size:
2360 786 661 102 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 1882 1912 2940 2989 3193 3942
Size: 1368 514 30 1028 49 204 749
Fragments arranged by size:
1368 1028 749 514 204 49 30
Bpu10I CC'TnA_GC
Cuts at: 0 1477 2053 2213 2278 2452 2599 3933 3942
Size: 1477 576 160 65 174 147 1334 9
Fragments arranged by size:
1477 1334 576 174 160 147 65 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 2614 3001 3187 3942
Size: 1117 1497 387 186 755
Fragments arranged by size:
1497 1117 755 387 186
BsaI GGTCTCn'nnnn_
Cuts at: 0 1417 2573 2677 3942
Size: 1417 1156 104 1265
Fragments arranged by size:
1417 1265 1156 104
BsaAI yAC'GTr
Cuts at: 0 2787 3942
Size: 2787 1155
BsaBI GATnn'nnATC
Cuts at: 0 3262 3942
Size: 3262 680
BsaHI Gr'CG_yC
Cuts at: 0 2866 2987 3327 3942
Size: 2866 121 340 615
Fragments arranged by size:
2866 615 340 121
BsaWI w'CCGG_w
Cuts at: 0 3617 3942
Size: 3617 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1880 1885 1910 1915 2785 2815 2913 2943
Size: 1880 5 25 5 870 30 98 30
Cuts at: 2943 3942
Size: 999
Fragments arranged by size:
1880 999 870 98 30 30 25 5
5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 247 414 496 931 1348 1435 1447
Size: 244 3 167 82 435 417 87 12
Cuts at: 1447 1762 1813 1948 2221 2400 2677 2845 3763
Size: 315 51 135 273 179 277 168 918
Cuts at: 3763 3921 3942
Size: 158 21
Fragments arranged by size:
918 435 417 315 277 273 244 179
168 167 158 135 87 82 51 21
12 3
BseYI C'CCAG_C
Cuts at: 0 150 336 702 834 1026 1944 1955 2190
Size: 150 186 366 132 192 918 11 235
Cuts at: 2190 2295 2998 3079 3489 3585 3942
Size: 105 703 81 410 96 357
Fragments arranged by size:
918 703 410 366 357 235 192 186
150 132 105 96 81 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 3490 3942
Size: 109 3381 452
Fragments arranged by size:
3381 452 109
BsiHKAI G_wGCw'C
Cuts at: 0 457 907 1013 1076 1289 1355 1408 1898
Size: 457 450 106 63 213 66 53 490
Cuts at: 1898 2101 2552 2628 2654 2669 3524 3942
Size: 203 451 76 26 15 855 418
Fragments arranged by size:
855 490 457 451 450 418 213 203
106 76 66 63 53 26 15
Bsp1286I G_dGCh'C
Cuts at: 0 437 457 658 825 907 1013 1048 1076
Size: 437 20 201 167 82 106 35 28
Cuts at: 1076 1289 1321 1355 1408 1898 2101 2380 2552
Size: 213 32 34 53 490 203 279 172
Cuts at: 2552 2628 2654 2669 2746 2892 3524 3584 3942
Size: 76 26 15 77 146 632 60 358
Fragments arranged by size:
632 490 437 358 279 213 203 201
172 167 146 106 82 77 76 60
53 35 34 32 28 26 20 15
BspHI T'CATG_A
Cuts at: 0 1013 2075 2264 3942
Size: 1013 1062 189 1678
Fragments arranged by size:
1678 1062 1013 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 341 1220 1811 1841 3226 3942
Size: 197 144 879 591 30 1385 716
Fragments arranged by size:
1385 879 716 591 197 144 30
BsrBI CCG'CTC
Cuts at: 0 374 1292 1437 3177 3942
Size: 374 918 145 1740 765
Fragments arranged by size:
1740 918 765 374 145
BsrDI GCAATG_nn'
Cuts at: 0 1275 3942
Size: 1275 2667
BsrFI r'CCGG_y
Cuts at: 0 16 782 1339 1676 2372 3194 3942
Size: 16 766 557 337 696 822 748
Fragments arranged by size:
822 766 748 696 557 337 16
BsrGI T'GTAC_A
Cuts at: 0 389 2270 3942
Size: 389 1881 1672
Fragments arranged by size:
1881 1672 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3727 3942
Size: 3727 215
BstEII G'GTnAC_C
Cuts at: 0 3396 3942
Size: 3396 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 3447 3805 3942
Size: 684 2763 358 137
Fragments arranged by size:
2763 684 358 137
BstYI r'GATC_y
Cuts at: 0 1202 1517 2903 3149 3531 3942
Size: 1202 315 1386 246 382 411
Fragments arranged by size:
1386 1202 411 382 315 246
Bsu36I CC'TnA_GG
Cuts at: 0 1234 3942
Size: 1234 2708
BtgI C'CryG_G
Cuts at: 0 731 1067 1910 2083 2846 3942
Size: 731 336 843 173 763 1096
Fragments arranged by size:
1096 843 763 731 336 173
BtsI GCAGTG_nn'
Cuts at: 0 656 857 1377 1990 3458 3596 3942
Size: 656 201 520 613 1468 138 346
Fragments arranged by size:
1468 656 613 520 346 201 138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3204 3239 3942
Size: 3204 35 703
Fragments arranged by size:
3204 703 35
DraIII CAC_nnn'GTG
Cuts at: 0 805 1370 3942
Size: 805 565 2572
Fragments arranged by size:
2572 805 565
DrdI GACnn_nn'nnGTC
Cuts at: 0 3232 3942
Size: 3232 710
EaeI y'GGCC_r
Cuts at: 0 1620 2606 2777 3807 3942
Size: 1620 986 171 1030 135
Fragments arranged by size:
1620 1030 986 171 135
EarI CTCTTCn'nnn_
Cuts at: 0 138 1105 1282 2675 3942
Size: 138 967 177 1393 1267
Fragments arranged by size:
1393 1267 967 177 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2359 3061 3942
Size: 2359 702 881
Fragments arranged by size:
2359 881 702
EcoICRI GAG'CTC
Cuts at: 0 905 1353 1896 2667 3942
Size: 905 448 543 771 1275
Fragments arranged by size:
1275 905 771 543 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1368 1543 1725 1882 1912 2940 2989 3193
Size: 1368 175 182 157 30 1028 49 204
Cuts at: 3193 3942
Size: 749
Fragments arranged by size:
1368 1028 749 204 182 175 157 49
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1924 3058 3942
Size: 1924 1134 884
Fragments arranged by size:
1924 1134 884
EcoO109I rG'GnC_Cy
Cuts at: 0 414 681 795 1080 1818 1971 2144 2772
Size: 414 267 114 285 738 153 173 628
Cuts at: 2772 3064 3753 3942
Size: 292 689 189
Fragments arranged by size:
738 689 628 414 292 285 267 189
173 153 114
EcoRI G'AATT_C
Cuts at: 0 3127 3942
Size: 3127 815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 280 2655 3879 3942
Size: 280 2375 1224 63
Fragments arranged by size:
2375 1224 280 63
FseI GG_CCGG'CC
Cuts at: 0 1343 3942
Size: 1343 2599
FspI TGC'GCA
Cuts at: 0 535 985 3942
Size: 535 450 2957
Fragments arranged by size:
2957 535 450
HaeII r_GCGC'y
Cuts at: 0 1812 1962 2869 3171 3627 3942
Size: 1812 150 907 302 456 315
Fragments arranged by size:
1812 907 456 315 302 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1134 1166 1885 1917 2445 2477
Size: 658 32 444 32 719 32 528 32
Cuts at: 2477 3942
Size: 1465
Fragments arranged by size:
1465 719 658 528 444 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1904 2411 3942
Size: 1904 507 1531
Fragments arranged by size:
1904 1531 507
HindIII A'AGCT_T
Cuts at: 0 1562 3942
Size: 1562 2380
KasI G'GCGC_C
Cuts at: 0 2865 3942
Size: 2865 1077
KpnI G_GTAC'C
Cuts at: 0 2943 3942
Size: 2943 999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 872 2050 3942
Size: 471 401 1178 1892
Fragments arranged by size:
1892 1178 471 401
MscI TGG'CCA
Cuts at: 0 2608 2779 3809 3942
Size: 2608 171 1030 133
Fragments arranged by size:
2608 1030 171 133
MslI CAynn'nnrTG
Cuts at: 0 385 1411 1492 1636 2174 3136 3271 3445
Size: 385 1026 81 144 538 962 135 174
Cuts at: 3445 3466 3517 3883 3942
Size: 21 51 366 59
Fragments arranged by size:
1026 962 538 385 366 174 144 135
81 59 51 21
MspA1I CmG'CkG
Cuts at: 0 464 580 1473 2062 2295 2880 3339 3370
Size: 464 116 893 589 233 585 459 31
Cuts at: 3370 3727 3937 3942
Size: 357 210 5
Fragments arranged by size:
893 589 585 464 459 357 233 210
116 31 5
NaeI GCC'GGC
Cuts at: 0 18 1341 3196 3942
Size: 18 1323 1855 746
Fragments arranged by size:
1855 1323 746 18
NarI GG'CG_CC
Cuts at: 0 2866 3942
Size: 2866 1076
NcoI C'CATG_G
Cuts at: 0 1910 2846 3942
Size: 1910 936 1096
Fragments arranged by size:
1910 1096 936
NgoMIV G'CCGG_C
Cuts at: 0 16 1339 3194 3942
Size: 16 1323 1855 748
Fragments arranged by size:
1855 1323 748 16
NspI r_CATG'y
Cuts at: 0 24 145 386 1635 3942
Size: 24 121 241 1249 2307
Fragments arranged by size:
2307 1249 241 121 24
PasI CC'CwG_GG
Cuts at: 0 429 2885 3942
Size: 429 2456 1057
Fragments arranged by size:
2456 1057 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 1083 1367 1370 2295 3592 3942
Size: 1083 284 3 925 1297 350
Fragments arranged by size:
1297 1083 925 350 284 3
PfoI T'CCnGG_A
Cuts at: 0 284 1082 2249 3942
Size: 284 798 1167 1693
Fragments arranged by size:
1693 1167 798 284
PpuMI rG'GwC_Cy
Cuts at: 0 414 795 1080 2144 3064 3753 3942
Size: 414 381 285 1064 920 689 189
Fragments arranged by size:
1064 920 689 414 381 285 189
PspOMI G'GGCC_C
Cuts at: 0 2888 3942
Size: 2888 1054
PstI C_TGCA'G
Cuts at: 0 794 1349 1607 1808 3035 3638 3942
Size: 794 555 258 201 1227 603 304
Fragments arranged by size:
1227 794 603 555 304 258 201
PvuII CAG'CTG
Cuts at: 0 464 2295 3339 3370 3727 3937 3942
Size: 464 1831 1044 31 357 210 5
Fragments arranged by size:
1831 1044 464 357 210 31 5
SacI G_AGCT'C
Cuts at: 0 907 1355 1898 2669 3942
Size: 907 448 543 771 1273
Fragments arranged by size:
1273 907 771 543 448
SapI GCTCTTCn'nnn_
Cuts at: 0 1105 3942
Size: 1105 2837
SbfI CC_TGCA'GG
Cuts at: 0 794 1349 3942
Size: 794 555 2593
Fragments arranged by size:
2593 794 555
ScaI AGT'ACT
Cuts at: 0 3924 3942
Size: 3924 18
SexAI A'CCwGG_T
Cuts at: 0 812 1076 1366 3347 3942
Size: 812 264 290 1981 595
Fragments arranged by size:
1981 812 595 290 264
SfcI C'TryA_G
Cuts at: 0 42 214 790 1198 1345 1507 1603 1804
Size: 42 172 576 408 147 162 96 201
Cuts at: 1804 2619 3031 3055 3634 3890 3942
Size: 815 412 24 579 256 52
Fragments arranged by size:
815 579 576 412 408 256 201 172
162 147 96 52 42 24
SfoI GGC'GCC
Cuts at: 0 2867 3942
Size: 2867 1075
SgrAI Cr'CCGG_yG
Cuts at: 0 2372 3942
Size: 2372 1570
SmlI C'TyrA_G
Cuts at: 0 1132 2629 2980 3202 3942
Size: 1132 1497 351 222 740
Fragments arranged by size:
1497 1132 740 351 222
StuI AGG'CCT
Cuts at: 0 1753 3942
Size: 1753 2189
StyI C'CwwG_G
Cuts at: 0 8 1910 2846 3942
Size: 8 1902 936 1096
Fragments arranged by size:
1902 1096 936 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1065 1191 2299 3093 3222 3942
Size: 1065 126 1108 794 129 720
Fragments arranged by size:
1108 1065 794 720 129 126
TatI w'GTAC_w
Cuts at: 0 389 1655 2270 3922 3942
Size: 389 1266 615 1652 20
Fragments arranged by size:
1652 1266 615 389 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1166 1198 1880 1912 2913 2945
Size: 1078 32 56 32 682 32 1001 32
Cuts at: 2945 3068 3100 3942
Size: 123 32 842
Fragments arranged by size:
1078 1001 842 682 123 56 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 1971 2904 3942
Size: 282 1689 933 1038
Fragments arranged by size:
1689 1038 933 282
XmnI GAAnn'nnTTC
Cuts at: 0 2428 3625 3942
Size: 2428 1197 317
Fragments arranged by size:
2428 1197 317
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SapI SbfI ScaI SexAI SfcI SfoI
SgrAI SmlI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AscI AseI AsiSI
AvrII BaeI BciVI BclI BfrBI BglII BmtI BplI
BsiEI BsiWI BsmI BsmBI BspEI BssHII BssSI BstBI
BstZ17I BtgZI ClaI DraI EagI EcoRV FspAI HpaI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI