Restriction map for
pF1KB9503
(Linear) (Six-base) MAPSORT of: KIBB9503.seq Check: 509 from: 1 to: 3804
KIBB9503.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AatII G_ACGT'C
Cuts at: 0 3395 3804
Size: 3395 409
AccI GT'mk_AC
Cuts at: 0 3176 3626 3804
Size: 3176 450 178
Fragments arranged by size:
3176 450 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1085 2501 3804
Size: 1085 1416 1303
Fragments arranged by size:
1416 1303 1085
AflIII A'CryG_T
Cuts at: 0 2277 3018 3804
Size: 2277 741 786
Fragments arranged by size:
2277 786 741
AleI CACnn'nnGTG
Cuts at: 0 47 232 3804
Size: 47 185 3572
Fragments arranged by size:
3572 185 47
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 967 3804
Size: 324 643 2837
Fragments arranged by size:
2837 643 324
AlwNI CAG_nnn'CTG
Cuts at: 0 460 1158 2148 2316 3334 3804
Size: 460 698 990 168 1018 470
Fragments arranged by size:
1018 990 698 470 460 168
ApaI G_GGCC'C
Cuts at: 0 938 1237 2113 2671 3796 3804
Size: 938 299 876 558 1125 8
Fragments arranged by size:
1125 938 876 558 299 8
ApoI r'AATT_y
Cuts at: 0 87 1595 1606 2218 2246 2647 2722 3075
Size: 87 1508 11 612 28 401 75 353
Cuts at: 3075 3466 3745 3804
Size: 391 279 59
Fragments arranged by size:
1508 612 401 391 353 279 87 75
59 28 11
AvaI C'yCGr_G
Cuts at: 0 2699 3804
Size: 2699 1105
BamHI G'GATC_C
Cuts at: 0 1586 2981 3804
Size: 1586 1395 823
Fragments arranged by size:
1586 1395 823
BanI G'GyrC_C
Cuts at: 0 598 1343 1363 2008 2551 2934 3027 3430
Size: 598 745 20 645 543 383 93 403
Cuts at: 3430 3660 3804
Size: 230 144
Fragments arranged by size:
745 645 598 543 403 383 230 144
93 20
BanII G_rGCy'C
Cuts at: 0 938 1237 2113 2671 3504 3536 3765 3796
Size: 938 299 876 558 833 32 229 31
Cuts at: 3796 3804
Size: 8
Fragments arranged by size:
938 876 833 558 299 229 32 31
8
BbeI G_GCGC'C
Cuts at: 0 2555 3804
Size: 2555 1249
BbsI GAAGACnn'nnnn_
Cuts at: 0 798 886 1220 2411 3804
Size: 798 88 334 1191 1393
Fragments arranged by size:
1393 1191 798 334 88
BbvCI CC'TCA_GC
Cuts at: 0 453 2297 3804
Size: 453 1844 1507
Fragments arranged by size:
1844 1507 453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1886 1920 2680 2714 3804
Size: 1886 34 760 34 1090
Fragments arranged by size:
1886 1090 760 34 34
BclI T'GATC_A
Cuts at: 0 2318 3804
Size: 2318 1486
BglII A'GATC_T
Cuts at: 0 1725 3804
Size: 1725 2079
Bme1580I G_kGCm'C
Cuts at: 0 171 938 1237 2113 2671 2939 3435 3796
Size: 171 767 299 876 558 268 496 361
Cuts at: 3796 3804
Size: 8
Fragments arranged by size:
876 767 558 496 361 299 268 171
8
BmgBI CAC'GTC
Cuts at: 0 3249 3600 3804
Size: 3249 351 204
Fragments arranged by size:
3249 351 204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 125 3804
Size: 125 3679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 271 833 995 1249 1513 2291 2389
Size: 203 68 562 162 254 264 778 98
Cuts at: 2389 2920 3124 3598 3804
Size: 531 204 474 206
Fragments arranged by size:
778 562 531 474 264 254 206 204
203 162 98 68
Bpu10I CC'TnA_GC
Cuts at: 0 453 833 2297 2615 3032 3764 3804
Size: 453 380 1464 318 417 732 40
Fragments arranged by size:
1464 732 453 417 380 318 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 985 2530 3391 3804
Size: 334 651 1545 861 413
Fragments arranged by size:
1545 861 651 413 334
BsaI GGTCTCn'nnnn_
Cuts at: 0 1867 1977 3804
Size: 1867 110 1827
Fragments arranged by size:
1867 1827 110
BsaAI yAC'GTr
Cuts at: 0 2278 3804
Size: 2278 1526
BsaBI GATnn'nnATC
Cuts at: 0 589 1321 1444 2323 3804
Size: 589 732 123 879 1481
Fragments arranged by size:
1481 879 732 589 123
BsaHI Gr'CG_yC
Cuts at: 0 2342 2552 3392 3804
Size: 2342 210 840 412
Fragments arranged by size:
2342 840 412 210
BsaWI w'CCGG_w
Cuts at: 0 791 1298 3233 3804
Size: 791 507 1935 571
Fragments arranged by size:
1935 791 571 507
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 968 998 2264 2294 3804
Size: 968 30 1266 30 1510
Fragments arranged by size:
1510 1266 968 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 268 880 1089 2056 2176 2752 3595
Size: 112 156 612 209 967 120 576 843
Cuts at: 3595 3804
Size: 209
Fragments arranged by size:
967 843 612 576 209 209 156 120
112
BseYI C'CCAG_C
Cuts at: 0 574 712 781 1638 2791 3639 3751 3804
Size: 574 138 69 857 1153 848 112 53
Fragments arranged by size:
1153 857 848 574 138 112 69 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1489 1975 3804
Size: 1489 486 1829
Fragments arranged by size:
1829 1489 486
BsiHKAI G_wGCw'C
Cuts at: 0 339 2277 3804
Size: 339 1938 1527
Fragments arranged by size:
1938 1527 339
BsmI GAATG_Cn'
Cuts at: 0 694 2585 3804
Size: 694 1891 1219
Fragments arranged by size:
1891 1219 694
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1101 3804
Size: 1101 2703
Bsp1286I G_dGCh'C
Cuts at: 0 171 339 938 1237 2113 2277 2671 2939
Size: 171 168 599 299 876 164 394 268
Cuts at: 2939 3435 3504 3536 3765 3796 3804
Size: 496 69 32 229 31 8
Fragments arranged by size:
876 599 496 394 299 268 229 171
168 164 69 32 31 8
BspHI T'CATG_A
Cuts at: 0 407 3804
Size: 407 3397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3612 3804
Size: 3612 192
BsrBI CCG'CTC
Cuts at: 0 1361 2030 3497 3804
Size: 1361 669 1467 307
Fragments arranged by size:
1467 1361 669 307
BsrDI GCAATG_nn'
Cuts at: 0 890 949 3804
Size: 890 59 2855
Fragments arranged by size:
2855 890 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 463 2148 3804
Size: 463 1685 1656
Fragments arranged by size:
1685 1656 463
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 2418 2779 3804
Size: 897 1521 361 1025
Fragments arranged by size:
1521 1025 897 361
BstYI r'GATC_y
Cuts at: 0 200 554 959 989 1530 1586 1725 2981
Size: 200 354 405 30 541 56 139 1256
Cuts at: 2981 3804
Size: 823
Fragments arranged by size:
1256 823 541 405 354 200 139 56
30
BstZ17I GTA'TAC
Cuts at: 0 3627 3804
Size: 3627 177
BtgI C'CryG_G
Cuts at: 0 2072 3435 3804
Size: 2072 1363 369
Fragments arranged by size:
2072 1363 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 469 693 1540 3036 3804
Size: 469 224 847 1496 768
Fragments arranged by size:
1496 847 768 469 224
BtsI GCAGTG_nn'
Cuts at: 0 902 3717 3804
Size: 902 2815 87
Fragments arranged by size:
2815 902 87
ClaI AT'CG_AT
Cuts at: 0 2655 3804
Size: 2655 1149
EaeI y'GGCC_r
Cuts at: 0 356 1160 1949 2862 3804
Size: 356 804 789 913 942
Fragments arranged by size:
942 913 804 789 356
EarI CTCTTCn'nnn_
Cuts at: 0 89 1351 1423 2477 3644 3804
Size: 89 1262 72 1054 1167 160
Fragments arranged by size:
1262 1167 1054 160 89 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2173 3804
Size: 2173 1631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 271 833 995 1085 1249 1513 2291
Size: 203 68 562 162 90 164 264 778
Cuts at: 2291 2389 2501 2920 3124 3598 3804
Size: 98 112 419 204 474 206
Fragments arranged by size:
778 562 474 419 264 206 204 203
164 162 112 98 90 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3668 3804
Size: 3668 136
EcoO109I rG'GnC_Cy
Cuts at: 0 1233 1234 1455 1672 2109 2110 2313 2604
Size: 1233 1 221 217 437 1 203 291
Cuts at: 2604 2668 3792 3793 3804
Size: 64 1124 1 11
Fragments arranged by size:
1233 1124 437 291 221 217 203 64
11 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3466 3804
Size: 3466 338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 144 3804
Size: 144 3660
FspI TGC'GCA
Cuts at: 0 1408 3804
Size: 1408 2396
FspAI rTGC'GCAy
Cuts at: 0 1408 3804
Size: 1408 2396
HaeII r_GCGC'y
Cuts at: 0 2555 3619 3804
Size: 2555 1064 185
Fragments arranged by size:
2555 1064 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 994 1026 1486 1518 2264 2296 2447 2479
Size: 994 32 460 32 746 32 151 32
Cuts at: 2479 2812 2844 3025 3057 3804
Size: 333 32 181 32 747
Fragments arranged by size:
994 747 746 460 333 181 151 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1061 2190 3804
Size: 1061 1129 1614
Fragments arranged by size:
1614 1129 1061
KasI G'GCGC_C
Cuts at: 0 2551 3804
Size: 2551 1253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 228 3218 3804
Size: 136 92 2990 586
Fragments arranged by size:
2990 586 136 92
MscI TGG'CCA
Cuts at: 0 358 1162 1951 3804
Size: 358 804 789 1853
Fragments arranged by size:
1853 804 789 358
MslI CAynn'nnrTG
Cuts at: 0 47 232 382 895 923 2416 2628 3804
Size: 47 185 150 513 28 1493 212 1176
Fragments arranged by size:
1493 1176 513 212 185 150 47 28
MspA1I CmG'CkG
Cuts at: 0 181 3399 3437 3804
Size: 181 3218 38 367
Fragments arranged by size:
3218 367 181 38
NarI GG'CG_CC
Cuts at: 0 2552 3804
Size: 2552 1252
NspI r_CATG'y
Cuts at: 0 1710 3022 3804
Size: 1710 1312 782
Fragments arranged by size:
1710 1312 782
PciI A'CATG_T
Cuts at: 0 3018 3804
Size: 3018 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 2388 2518 3804
Size: 2388 130 1286
Fragments arranged by size:
2388 1286 130
PfoI T'CCnGG_A
Cuts at: 0 2771 3804
Size: 2771 1033
PmlI CAC'GTG
Cuts at: 0 2278 3804
Size: 2278 1526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 3804
Size: 321 32 3451
Fragments arranged by size:
3451 321 32
PpuMI rG'GwC_Cy
Cuts at: 0 1455 1672 2313 2604 3804
Size: 1455 217 641 291 1200
Fragments arranged by size:
1455 1200 641 291 217
PshAI GACnn'nnGTC
Cuts at: 0 1066 3804
Size: 1066 2738
PspOMI G'GGCC_C
Cuts at: 0 934 1233 2109 2667 3792 3804
Size: 934 299 876 558 1125 12
Fragments arranged by size:
1125 934 876 558 299 12
PstI C_TGCA'G
Cuts at: 0 443 3041 3623 3804
Size: 443 2598 582 181
Fragments arranged by size:
2598 582 443 181
SacII CC_GC'GG
Cuts at: 0 3438 3804
Size: 3438 366
SanDI GG'GwC_CC
Cuts at: 0 1455 3804
Size: 1455 2349
SapI GCTCTTCn'nnn_
Cuts at: 0 1351 1423 3804
Size: 1351 72 2381
Fragments arranged by size:
2381 1351 72
SfcI C'TryA_G
Cuts at: 0 439 1238 3037 3121 3173 3619 3804
Size: 439 799 1799 84 52 446 185
Fragments arranged by size:
1799 799 446 439 185 84 52
SfoI GGC'GCC
Cuts at: 0 2553 3804
Size: 2553 1251
SmlI C'TyrA_G
Cuts at: 0 313 964 2545 3370 3804
Size: 313 651 1581 825 434
Fragments arranged by size:
1581 825 651 434 313
StuI AGG'CCT
Cuts at: 0 371 451 2374 3804
Size: 371 80 1923 1430
Fragments arranged by size:
1923 1430 371 80
StyI C'CwwG_G
Cuts at: 0 366 938 1076 1526 2012 2555 2833 2888
Size: 366 572 138 450 486 543 278 55
Cuts at: 2888 3089 3656 3787 3804
Size: 201 567 131 17
Fragments arranged by size:
572 567 543 486 450 366 278 201
138 131 55 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 3352 3804
Size: 795 2557 452
Fragments arranged by size:
2557 795 452
TatI w'GTAC_w
Cuts at: 0 1391 2755 3804
Size: 1391 1364 1049
Fragments arranged by size:
1391 1364 1049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1347 1379 3804
Size: 1347 32 2425
Fragments arranged by size:
2425 1347 32
Tth111I GACn'n_nGTC
Cuts at: 0 1657 2344 2536 3804
Size: 1657 687 192 1268
Fragments arranged by size:
1657 1268 687 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 2562 2895 3675 3804
Size: 178 2384 333 780 129
Fragments arranged by size:
2384 780 333 178 129
ZraI GAC'GTC
Cuts at: 0 3393 3804
Size: 3393 411
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglII Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BstAPI BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NarI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI SacII
SanDI SapI SfcI SfoI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BciVI BfrBI
BglI BlpI BmrI BmtI BsiEI BsiWI BspEI BsrFI
BsrGI BssHII BssSI BstBI BstEII Bsu36I CspCI DraI
DraIII DrdI EagI EcoICRI EcoRV FseI HindIII HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmaI XmnI