Restriction map for
pF1KB9502
(Linear) (Six-base) MAPSORT of: KIBB9502.seq Check: 5487 from: 1 to: 3519
KIBB9502.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 381 2269 2876 3519
Size: 381 1888 607 643
Fragments arranged by size:
1888 643 607 381
Acc65I G'GTAC_C
Cuts at: 0 1288 3507 3519
Size: 1288 2219 12
Fragments arranged by size:
2219 1288 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1159 1351 1592 1700 1715 2502 2608
Size: 953 206 192 241 108 15 787 106
Cuts at: 2608 3519
Size: 911
Fragments arranged by size:
953 911 787 241 206 192 108 106
15
AflIII A'CryG_T
Cuts at: 0 191 1974 3519
Size: 191 1783 1545
Fragments arranged by size:
1783 1545 191
AleI CACnn'nnGTG
Cuts at: 0 2932 3519
Size: 2932 587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 111 143 3519
Size: 111 32 3376
Fragments arranged by size:
3376 111 32
AlwNI CAG_nnn'CTG
Cuts at: 0 616 3519
Size: 616 2903
ApaLI G'TGCA_C
Cuts at: 0 1772 3519
Size: 1772 1747
ApoI r'AATT_y
Cuts at: 0 703 792 991 1117 1230 1616 1752 2167
Size: 703 89 199 126 113 386 136 415
Cuts at: 2167 2179 2255 3519
Size: 12 76 1264
Fragments arranged by size:
1264 703 415 386 199 136 126 113
89 76 12
AvaI C'yCGr_G
Cuts at: 0 51 3519
Size: 51 3468
BamHI G'GATC_C
Cuts at: 0 2543 3519
Size: 2543 976
BanI G'GyrC_C
Cuts at: 0 309 671 1221 1288 2934 3507 3519
Size: 309 362 550 67 1646 573 12
Fragments arranged by size:
1646 573 550 362 309 67 12
BanII G_rGCy'C
Cuts at: 0 328 3519
Size: 328 3191
BbsI GAAGACnn'nnnn_
Cuts at: 0 1147 2036 2078 2129 2648 3220 3519
Size: 1147 889 42 51 519 572 299
Fragments arranged by size:
1147 889 572 519 299 51 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 1705 3431 3519
Size: 1705 1726 88
Fragments arranged by size:
1726 1705 88
BclI T'GATC_A
Cuts at: 0 2208 3519
Size: 2208 1311
BglI GCCn_nnn'nGGC
Cuts at: 0 24 3519
Size: 24 3495
BglII A'GATC_T
Cuts at: 0 1029 1948 2599 3519
Size: 1029 919 651 920
Fragments arranged by size:
1029 920 919 651
Bme1580I G_kGCm'C
Cuts at: 0 1776 3519
Size: 1776 1743
BmgBI CAC'GTC
Cuts at: 0 394 3519
Size: 394 3125
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 703 3519
Size: 260 443 2816
Fragments arranged by size:
2816 443 260
BmtI G_CTAG'C
Cuts at: 0 1361 1388 3519
Size: 1361 27 2131
Fragments arranged by size:
2131 1361 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1533 3519
Size: 1533 1986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 2635 2952 3519
Size: 621 2014 317 567
Fragments arranged by size:
2014 621 567 317
Bpu10I CC'TnA_GC
Cuts at: 0 646 3519
Size: 646 2873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 915 1102 3519
Size: 915 187 2417
Fragments arranged by size:
2417 915 187
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 3519
Size: 75 3444
BsaAI yAC'GTr
Cuts at: 0 3025 3519
Size: 3025 494
BsaBI GATnn'nnATC
Cuts at: 0 563 2213 3519
Size: 563 1650 1306
Fragments arranged by size:
1650 1306 563
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 1647 1677 3519
Size: 469 30 1148 30 1842
Fragments arranged by size:
1842 1148 469 30 30
BseYI C'CCAG_C
Cuts at: 0 45 3519
Size: 45 3474
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2491 3166 3519
Size: 2491 675 353
Fragments arranged by size:
2491 675 353
BsiHKAI G_wGCw'C
Cuts at: 0 365 1485 1776 2162 3519
Size: 365 1120 291 386 1357
Fragments arranged by size:
1357 1120 386 365 291
BsmI GAATG_Cn'
Cuts at: 0 1539 3519
Size: 1539 1980
Bsp1286I G_dGCh'C
Cuts at: 0 328 365 1485 1776 2162 3519
Size: 328 37 1120 291 386 1357
Fragments arranged by size:
1357 1120 386 328 291 37
BspHI T'CATG_A
Cuts at: 0 344 537 3495 3519
Size: 344 193 2958 24
Fragments arranged by size:
2958 344 193 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1276 1753 3108 3519
Size: 1276 477 1355 411
Fragments arranged by size:
1355 1276 477 411
BsrGI T'GTAC_A
Cuts at: 0 2770 3519
Size: 2770 749
BstEII G'GTnAC_C
Cuts at: 0 2791 3519
Size: 2791 728
BstYI r'GATC_y
Cuts at: 0 940 1029 1948 2543 2599 3519
Size: 940 89 919 595 56 920
Fragments arranged by size:
940 920 919 595 89 56
BstZ17I GTA'TAC
Cuts at: 0 382 2270 3519
Size: 382 1888 1249
Fragments arranged by size:
1888 1249 382
BtgI C'CryG_G
Cuts at: 0 2539 3519
Size: 2539 980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3118 3519
Size: 3118 401
BtsI GCAGTG_nn'
Cuts at: 0 1776 3519
Size: 1776 1743
ClaI AT'CG_AT
Cuts at: 0 504 3519
Size: 504 3015
DraI TTT'AAA
Cuts at: 0 2744 2835 3519
Size: 2744 91 684
Fragments arranged by size:
2744 684 91
DraIII CAC_nnn'GTG
Cuts at: 0 1150 3339 3519
Size: 1150 2189 180
Fragments arranged by size:
2189 1150 180
EaeI y'GGCC_r
Cuts at: 0 1245 2536 3519
Size: 1245 1291 983
Fragments arranged by size:
1291 1245 983
EarI CTCTTCn'nnn_
Cuts at: 0 951 1212 1490 1844 2150 3519
Size: 951 261 278 354 306 1369
Fragments arranged by size:
1369 951 354 306 278 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 953 1159 1351 1592 1700 1715 2502
Size: 621 332 206 192 241 108 15 787
Cuts at: 2502 2608 2635 2952 3519
Size: 106 27 317 567
Fragments arranged by size:
787 621 567 332 317 241 206 192
108 106 27 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1733 3519
Size: 1733 1786
EcoO109I rG'GnC_Cy
Cuts at: 0 1627 3519
Size: 1627 1892
EcoRI G'AATT_C
Cuts at: 0 792 3519
Size: 792 2727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 962 2392 3519
Size: 962 1430 1127
Fragments arranged by size:
1430 1127 962
HaeII r_GCGC'y
Cuts at: 0 12 3519
Size: 12 3507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 230 262 469 501 2584 2616 3032 3064
Size: 230 32 207 32 2083 32 416 32
Cuts at: 3064 3519
Size: 455
Fragments arranged by size:
2083 455 416 230 207 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 338 3070 3519
Size: 338 2732 449
Fragments arranged by size:
2732 449 338
KpnI G_GTAC'C
Cuts at: 0 1292 3511 3519
Size: 1292 2219 8
Fragments arranged by size:
2219 1292 8
MfeI C'AATT_G
Cuts at: 0 2192 3519
Size: 2192 1327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 3519
Size: 176 3343
MscI TGG'CCA
Cuts at: 0 1247 2538 3519
Size: 1247 1291 981
Fragments arranged by size:
1291 1247 981
MslI CAynn'nnrTG
Cuts at: 0 2219 2932 3092 3519
Size: 2219 713 160 427
Fragments arranged by size:
2219 713 427 160
MspA1I CmG'CkG
Cuts at: 0 109 2569 2856 3003 3519
Size: 109 2460 287 147 516
Fragments arranged by size:
2460 516 287 147 109
NcoI C'CATG_G
Cuts at: 0 2539 3519
Size: 2539 980
NheI G'CTAG_C
Cuts at: 0 1357 1384 3519
Size: 1357 27 2135
Fragments arranged by size:
2135 1357 27
NspI r_CATG'y
Cuts at: 0 18 195 1978 3370 3519
Size: 18 177 1783 1392 149
Fragments arranged by size:
1783 1392 177 149 18
PciI A'CATG_T
Cuts at: 0 191 1974 3519
Size: 191 1783 1545
Fragments arranged by size:
1783 1545 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 1444 3519
Size: 1444 2075
PfoI T'CCnGG_A
Cuts at: 0 1983 3519
Size: 1983 1536
PmlI CAC'GTG
Cuts at: 0 3025 3519
Size: 3025 494
PpuMI rG'GwC_Cy
Cuts at: 0 1627 3519
Size: 1627 1892
PsiI TTA'TAA
Cuts at: 0 1123 2705 3519
Size: 1123 1582 814
Fragments arranged by size:
1582 1123 814
PstI C_TGCA'G
Cuts at: 0 108 733 1215 1710 1911 2057 3119 3519
Size: 108 625 482 495 201 146 1062 400
Fragments arranged by size:
1062 625 495 482 400 201 146 108
PvuII CAG'CTG
Cuts at: 0 2569 2856 3003 3519
Size: 2569 287 147 516
Fragments arranged by size:
2569 516 287 147
SapI GCTCTTCn'nnn_
Cuts at: 0 1212 1490 2150 3519
Size: 1212 278 660 1369
Fragments arranged by size:
1369 1212 660 278
SexAI A'CCwGG_T
Cuts at: 0 1146 3519
Size: 1146 2373
SfcI C'TryA_G
Cuts at: 0 104 729 1211 1544 1706 1907 1913 2053
Size: 104 625 482 333 162 201 6 140
Cuts at: 2053 2312 3115 3519
Size: 259 803 404
Fragments arranged by size:
803 625 482 404 333 259 201 162
140 104 6
SmaI CCC'GGG
Cuts at: 0 53 3519
Size: 53 3466
SmlI C'TyrA_G
Cuts at: 0 930 1081 3519
Size: 930 151 2438
Fragments arranged by size:
2438 930 151
SphI G_CATG'C
Cuts at: 0 18 3519
Size: 18 3501
SspI AAT'ATT
Cuts at: 0 424 2231 3519
Size: 424 1807 1288
Fragments arranged by size:
1807 1288 424
StyI C'CwwG_G
Cuts at: 0 853 1178 1292 1937 2273 2539 3519
Size: 853 325 114 645 336 266 980
Fragments arranged by size:
980 853 645 336 325 266 114
TatI w'GTAC_w
Cuts at: 0 2128 2482 2770 3519
Size: 2128 354 288 749
Fragments arranged by size:
2128 749 354 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2357 2389 2673 2705 3009 3041 3519
Size: 2357 32 284 32 304 32 478
Fragments arranged by size:
2357 478 304 284 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3427 3519
Size: 3427 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 373 2537 3519
Size: 373 2164 982
Fragments arranged by size:
2164 982 373
XmaI C'CCGG_G
Cuts at: 0 51 3519
Size: 51 3468
XmnI GAAnn'nnTTC
Cuts at: 0 796 991 3095 3519
Size: 796 195 2104 424
Fragments arranged by size:
2104 796 424 195
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BciVI BclI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NheI NspI PciI PflMI PfoI PmlI PpuMI PsiI
PstI PvuII SapI SexAI SfcI SmaI SmlI SphI
SspI StyI TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BfrBI BlpI BsaHI BsaWI BseRI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI Bsu36I CspCI DrdI EagI EciI EcoICRI
EcoRV FseI FspI FspAI HincII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI
PasI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
TaqII XbaI XhoI ZraI