Restriction map for
pF1KB9501
(Linear) (Six-base) MAPSORT of: KIBB9501.seq Check: 6902 from: 1 to: 3360
KIBB9501.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 235 277 583 1040 1240 1297 1427
Size: 191 44 42 306 457 200 57 130
Cuts at: 1427 2422 3360
Size: 995 938
Fragments arranged by size:
995 938 457 306 200 191 130 57
44 42
AflIII A'CryG_T
Cuts at: 0 2894 3360
Size: 2894 466
AhdI GACnn_n'nnGTC
Cuts at: 0 3313 3360
Size: 3313 47
AleI CACnn'nnGTG
Cuts at: 0 2153 3360
Size: 2153 1207
ApaLI G'TGCA_C
Cuts at: 0 397 3360
Size: 397 2963
ApoI r'AATT_y
Cuts at: 0 689 794 1929 2617 3182 3360
Size: 689 105 1135 688 565 178
Fragments arranged by size:
1135 689 688 565 178 105
AvrII C'CTAG_G
Cuts at: 0 1875 3360
Size: 1875 1485
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1812 1845 3360
Size: 1812 33 1515
Fragments arranged by size:
1812 1515 33
BamHI G'GATC_C
Cuts at: 0 1373 3360
Size: 1373 1987
BanI G'GyrC_C
Cuts at: 0 1794 2135 2317 3003 3360
Size: 1794 341 182 686 357
Fragments arranged by size:
1794 686 357 341 182
BanII G_rGCy'C
Cuts at: 0 926 1964 2330 3360
Size: 926 1038 366 1030
Fragments arranged by size:
1038 1030 926 366
BbeI G_GCGC'C
Cuts at: 0 2139 3360
Size: 2139 1221
BbsI GAAGACnn'nnnn_
Cuts at: 0 2990 3309 3360
Size: 2990 319 51
Fragments arranged by size:
2990 319 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2806 2840 3360
Size: 2806 34 520
Fragments arranged by size:
2806 520 34
BclI T'GATC_A
Cuts at: 0 1121 1242 3360
Size: 1121 121 2118
Fragments arranged by size:
2118 1121 121
BglI GCCn_nnn'nGGC
Cuts at: 0 2049 3360
Size: 2049 1311
BglII A'GATC_T
Cuts at: 0 174 436 2229 2282 3360
Size: 174 262 1793 53 1078
Fragments arranged by size:
1793 1078 262 174 53
Bme1580I G_kGCm'C
Cuts at: 0 401 3360
Size: 401 2959
BmrI ACTGGGnnnn_n'
Cuts at: 0 419 572 797 1848 2030 3360
Size: 419 153 225 1051 182 1330
Fragments arranged by size:
1330 1051 419 225 182 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1757 1772 1896 2031 3289 3343 3360
Size: 1757 15 124 135 1258 54 17
Fragments arranged by size:
1757 1258 135 124 54 17 15
Bpu10I CC'TnA_GC
Cuts at: 0 3089 3360
Size: 3089 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 619 628 1576 3360
Size: 98 521 9 948 1784
Fragments arranged by size:
1784 948 521 98 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 502 2620 3360
Size: 502 2118 740
Fragments arranged by size:
2118 740 502
BsaBI GATnn'nnATC
Cuts at: 0 304 1564 2386 2513 3360
Size: 304 1260 822 127 847
Fragments arranged by size:
1260 847 822 304 127
BsaHI Gr'CG_yC
Cuts at: 0 2136 3360
Size: 2136 1224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 110 140 713 743 2064 2094 2420 2450
Size: 110 30 573 30 1321 30 326 30
Cuts at: 2450 2693 2709 2723 2739 3360
Size: 243 16 14 16 621
Fragments arranged by size:
1321 621 573 326 243 110 30 30
30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 676 2589 3360
Size: 35 641 1913 771
Fragments arranged by size:
1913 771 641 35
BseYI C'CCAG_C
Cuts at: 0 83 3193 3360
Size: 83 3110 167
Fragments arranged by size:
3110 167 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2177 2519 2957 3360
Size: 2177 342 438 403
Fragments arranged by size:
2177 438 403 342
BsiHKAI G_wGCw'C
Cuts at: 0 401 926 3360
Size: 401 525 2434
Fragments arranged by size:
2434 525 401
BsmI GAATG_Cn'
Cuts at: 0 78 3265 3360
Size: 78 3187 95
Fragments arranged by size:
3187 95 78
Bsp1286I G_dGCh'C
Cuts at: 0 401 926 1964 2330 3360
Size: 401 525 1038 366 1030
Fragments arranged by size:
1038 1030 525 401 366
BspHI T'CATG_A
Cuts at: 0 1832 3360
Size: 1832 1528
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 629 2152 3360
Size: 629 1523 1208
Fragments arranged by size:
1523 1208 629
BsrDI GCAATG_nn'
Cuts at: 0 3340 3360
Size: 3340 20
BsrFI r'CCGG_y
Cuts at: 0 898 3360
Size: 898 2462
BsrGI T'GTAC_A
Cuts at: 0 287 3360
Size: 287 3073
BssSI C'ACGA_G
Cuts at: 0 2353 3360
Size: 2353 1007
BstAPI GCAn_nnn'nTGC
Cuts at: 0 682 1438 2014 2317 3218 3360
Size: 682 756 576 303 901 142
Fragments arranged by size:
901 756 682 576 303 142
BstEII G'GTnAC_C
Cuts at: 0 351 951 3360
Size: 351 600 2409
Fragments arranged by size:
2409 600 351
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 1310 3360
Size: 963 347 2050
Fragments arranged by size:
2050 963 347
BstYI r'GATC_y
Cuts at: 0 174 436 753 1373 1559 2229 2282 3360
Size: 174 262 317 620 186 670 53 1078
Fragments arranged by size:
1078 670 620 317 262 186 174 53
BtsI GCAGTG_nn'
Cuts at: 0 1635 2945 3078 3360
Size: 1635 1310 133 282
Fragments arranged by size:
1635 1310 282 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 435 470 3360
Size: 435 35 2890
Fragments arranged by size:
2890 435 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2119 3360
Size: 2119 1241
EaeI y'GGCC_r
Cuts at: 0 227 1632 3360
Size: 227 1405 1728
Fragments arranged by size:
1728 1405 227
EarI CTCTTCn'nnn_
Cuts at: 0 664 931 1052 2093 2782 3360
Size: 664 267 121 1041 689 578
Fragments arranged by size:
1041 689 664 578 267 121
EcoICRI GAG'CTC
Cuts at: 0 924 3360
Size: 924 2436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 235 277 583 1040 1240 1297 1427
Size: 191 44 42 306 457 200 57 130
Cuts at: 1427 1757 1772 1896 2031 2422 3289 3343 3360
Size: 330 15 124 135 391 867 54 17
Fragments arranged by size:
867 457 391 330 306 200 191 135
130 124 57 54 44 42 17 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 270 2908 3360
Size: 116 154 2638 452
Fragments arranged by size:
2638 452 154 116
EcoRV GAT'ATC
Cuts at: 0 144 3360
Size: 144 3216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 615 3360
Size: 282 333 2745
Fragments arranged by size:
2745 333 282
FspI TGC'GCA
Cuts at: 0 655 3360
Size: 655 2705
HaeII r_GCGC'y
Cuts at: 0 2139 3360
Size: 2139 1221
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 264 296 335 367 421 453 1239 1271
Size: 264 32 39 32 54 32 786 32
Cuts at: 1271 1332 1364 1849 1881 2420 2452 2617 2649
Size: 61 32 485 32 539 32 165 32
Cuts at: 2649 2693 2725 3069 3101 3299 3331 3360
Size: 44 32 344 32 198 32 29
Fragments arranged by size:
786 539 485 344 264 198 165 61
54 44 39 32 32 32 32 32
32 32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 0 714 1206 3030 3360
Size: 714 492 1824 330
Fragments arranged by size:
1824 714 492 330
HindIII A'AGCT_T
Cuts at: 0 1546 3360
Size: 1546 1814
HpaI GTT'AAC
Cuts at: 0 3030 3360
Size: 3030 330
KasI G'GCGC_C
Cuts at: 0 2135 3360
Size: 2135 1225
MfeI C'AATT_G
Cuts at: 0 2636 3360
Size: 2636 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2406 3360
Size: 743 1663 954
Fragments arranged by size:
1663 954 743
MscI TGG'CCA
Cuts at: 0 229 3360
Size: 229 3131
MslI CAynn'nnrTG
Cuts at: 0 2153 2353 3360
Size: 2153 200 1007
Fragments arranged by size:
2153 1007 200
MspA1I CmG'CkG
Cuts at: 0 87 255 377 963 1571 2314 3360
Size: 87 168 122 586 608 743 1046
Fragments arranged by size:
1046 743 608 586 168 122 87
NarI GG'CG_CC
Cuts at: 0 2136 3360
Size: 2136 1224
NspI r_CATG'y
Cuts at: 0 633 654 1750 2898 3360
Size: 633 21 1096 1148 462
Fragments arranged by size:
1148 1096 633 462 21
PciI A'CATG_T
Cuts at: 0 2894 3360
Size: 2894 466
PfoI T'CCnGG_A
Cuts at: 0 1496 3320 3360
Size: 1496 1824 40
Fragments arranged by size:
1824 1496 40
PshAI GACnn'nnGTC
Cuts at: 0 34 3360
Size: 34 3326
PstI C_TGCA'G
Cuts at: 0 1155 3073 3360
Size: 1155 1918 287
Fragments arranged by size:
1918 1155 287
PvuII CAG'CTG
Cuts at: 0 87 255 377 963 2314 3360
Size: 87 168 122 586 1351 1046
Fragments arranged by size:
1351 1046 586 168 122 87
SacI G_AGCT'C
Cuts at: 0 926 3360
Size: 926 2434
SapI GCTCTTCn'nnn_
Cuts at: 0 664 931 2782 3360
Size: 664 267 1851 578
Fragments arranged by size:
1851 664 578 267
ScaI AGT'ACT
Cuts at: 0 2335 3360
Size: 2335 1025
SfcI C'TryA_G
Cuts at: 0 1151 3069 3360
Size: 1151 1918 291
Fragments arranged by size:
1918 1151 291
SfoI GGC'GCC
Cuts at: 0 2137 3360
Size: 2137 1223
SmlI C'TyrA_G
Cuts at: 0 113 634 643 1555 3360
Size: 113 521 9 912 1805
Fragments arranged by size:
1805 912 521 113 9
SphI G_CATG'C
Cuts at: 0 633 654 3360
Size: 633 21 2706
Fragments arranged by size:
2706 633 21
SspI AAT'ATT
Cuts at: 0 1159 2869 3360
Size: 1159 1710 491
Fragments arranged by size:
1710 1159 491
StuI AGG'CCT
Cuts at: 0 2052 3360
Size: 2052 1308
StyI C'CwwG_G
Cuts at: 0 1875 3240 3360
Size: 1875 1365 120
Fragments arranged by size:
1875 1365 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3021 3360
Size: 3021 339
TatI w'GTAC_w
Cuts at: 0 287 2333 3360
Size: 287 2046 1027
Fragments arranged by size:
2046 1027 287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 110 142 713 745 2709 2741 3360
Size: 110 32 571 32 1964 32 619
Fragments arranged by size:
1964 619 571 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2104 3360
Size: 2104 1256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2480 2847 3360
Size: 2480 367 513
Fragments arranged by size:
2480 513 367
XmnI GAAnn'nnTTC
Cuts at: 0 176 3360
Size: 176 3184
Enzymes that do cut:
AcuI AflIII AhdI AleI ApaLI ApoI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BclI BglI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI BtsI CspCI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I
NarI NspI PciI PfoI PshAI PstI PvuII SacI
SapI ScaI SfcI SfoI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
BbvCI BciVI BfrBI BlpI BmgBI BmtI BplI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII EagI
EciI EcoO109I EcoRI FseI FspAI KpnI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI XbaI XhoI XmaI ZraI