Restriction map for
pF1KB9500
(Linear) (Six-base) MAPSORT of: KIBB9500.seq Check: 4154 from: 1 to: 2025
KIBB9500.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
Acc65I G'GTAC_C
Cuts at: 0 2004 2025
Size: 2004 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1874 2025
Size: 1874 151
AflII C'TTAA_G
Cuts at: 0 259 2025
Size: 259 1766
AhdI GACnn_n'nnGTC
Cuts at: 0 960 2025
Size: 960 1065
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 884 916 2025
Size: 884 32 1109
Fragments arranged by size:
1109 884 32
ApaLI G'TGCA_C
Cuts at: 0 158 2025
Size: 158 1867
ApoI r'AATT_y
Cuts at: 0 275 923 1648 1659 1758 1932 2025
Size: 275 648 725 11 99 174 93
Fragments arranged by size:
725 648 275 174 99 93 11
AseI AT'TA_AT
Cuts at: 0 911 2025
Size: 911 1114
BanI G'GyrC_C
Cuts at: 0 130 2004 2025
Size: 130 1874 21
Fragments arranged by size:
1874 130 21
BanII G_rGCy'C
Cuts at: 0 707 2025
Size: 707 1318
BbsI GAAGACnn'nnnn_
Cuts at: 0 380 1720 1753 1885 2025
Size: 380 1340 33 132 140
Fragments arranged by size:
1340 380 140 132 33
BbvCI CC'TCA_GC
Cuts at: 0 2020 2025
Size: 2020 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 661 695 2025
Size: 661 34 1330
Fragments arranged by size:
1330 661 34
BclI T'GATC_A
Cuts at: 0 1142 1697 2025
Size: 1142 555 328
Fragments arranged by size:
1142 555 328
BfrBI ATG'CAT
Cuts at: 0 484 972 2025
Size: 484 488 1053
Fragments arranged by size:
1053 488 484
BglII A'GATC_T
Cuts at: 0 561 1385 2025
Size: 561 824 640
Fragments arranged by size:
824 640 561
BlpI GC'TnA_GC
Cuts at: 0 683 2025
Size: 683 1342
Bme1580I G_kGCm'C
Cuts at: 0 133 162 2025
Size: 133 29 1863
Fragments arranged by size:
1863 133 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 995 2025
Size: 995 1030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 2025
Size: 140 1885
Bpu10I CC'TnA_GC
Cuts at: 0 1966 2020 2025
Size: 1966 54 5
Fragments arranged by size:
1966 54 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 2025
Size: 554 1471
BsaI GGTCTCn'nnnn_
Cuts at: 0 1431 1550 2025
Size: 1431 119 475
Fragments arranged by size:
1431 475 119
BsaWI w'CCGG_w
Cuts at: 0 289 1754 2025
Size: 289 1465 271
Fragments arranged by size:
1465 289 271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 743 773 884 914 2025
Size: 743 30 111 30 1111
Fragments arranged by size:
1111 743 111 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 764 1396 2025
Size: 764 632 629
Fragments arranged by size:
764 632 629
BsiHKAI G_wGCw'C
Cuts at: 0 162 2025
Size: 162 1863
BsmI GAATG_Cn'
Cuts at: 0 486 1603 2025
Size: 486 1117 422
Fragments arranged by size:
1117 486 422
Bsp1286I G_dGCh'C
Cuts at: 0 133 162 707 2025
Size: 133 29 545 1318
Fragments arranged by size:
1318 545 133 29
BspEI T'CCGG_A
Cuts at: 0 1754 2025
Size: 1754 271
BspHI T'CATG_A
Cuts at: 0 1496 2025
Size: 1496 529
BsrDI GCAATG_nn'
Cuts at: 0 1787 2025
Size: 1787 238
BsrFI r'CCGG_y
Cuts at: 0 1570 2025
Size: 1570 455
BssSI C'ACGA_G
Cuts at: 0 785 2025
Size: 785 1240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 693 2025
Size: 693 1332
BstXI CCAn_nnnn'nTGG
Cuts at: 0 19 1166 2015 2025
Size: 19 1147 849 10
Fragments arranged by size:
1147 849 19 10
BstYI r'GATC_y
Cuts at: 0 561 1155 1385 2025
Size: 561 594 230 640
Fragments arranged by size:
640 594 561 230
BtsI GCAGTG_nn'
Cuts at: 0 442 2025
Size: 442 1583
EaeI y'GGCC_r
Cuts at: 0 738 1470 1826 1905 2025
Size: 738 732 356 79 120
Fragments arranged by size:
738 732 356 120 79
EarI CTCTTCn'nnn_
Cuts at: 0 1324 1429 1850 2025
Size: 1324 105 421 175
Fragments arranged by size:
1324 421 175 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 1874 2025
Size: 140 1734 151
Fragments arranged by size:
1734 151 140
EcoRI G'AATT_C
Cuts at: 0 1758 1932 2025
Size: 1758 174 93
Fragments arranged by size:
1758 174 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1533 1828 2025
Size: 1533 295 197
Fragments arranged by size:
1533 295 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 946 978 987 1019 1390 1422
Size: 55 32 859 32 9 32 371 32
Cuts at: 1422 1430 1462 2025
Size: 8 32 563
Fragments arranged by size:
859 563 371 55 32 32 32 32
32 9 8
HincII GTy'rAC
Cuts at: 0 111 1006 2025
Size: 111 895 1019
Fragments arranged by size:
1019 895 111
HindIII A'AGCT_T
Cuts at: 0 1230 2025
Size: 1230 795
KpnI G_GTAC'C
Cuts at: 0 2008 2025
Size: 2008 17
MfeI C'AATT_G
Cuts at: 0 1925 2025
Size: 1925 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 426 575 2025
Size: 146 280 149 1450
Fragments arranged by size:
1450 280 149 146
MscI TGG'CCA
Cuts at: 0 740 1472 1828 1907 2025
Size: 740 732 356 79 118
Fragments arranged by size:
740 732 356 118 79
MslI CAynn'nnrTG
Cuts at: 0 17 136 2025
Size: 17 119 1889
Fragments arranged by size:
1889 119 17
MspA1I CmG'CkG
Cuts at: 0 1624 2025
Size: 1624 401
NdeI CA'TA_TG
Cuts at: 0 14 2025
Size: 14 2011
NsiI A_TGCA'T
Cuts at: 0 486 974 2025
Size: 486 488 1051
Fragments arranged by size:
1051 488 486
NspI r_CATG'y
Cuts at: 0 28 2025
Size: 28 1997
PacI TTA_AT'TAA
Cuts at: 0 1976 2025
Size: 1976 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 884 916 2025
Size: 884 32 1109
Fragments arranged by size:
1109 884 32
PshAI GACnn'nnGTC
Cuts at: 0 988 2025
Size: 988 1037
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 752 784 2025
Size: 752 32 1241
Fragments arranged by size:
1241 752 32
PstI C_TGCA'G
Cuts at: 0 867 1530 2025
Size: 867 663 495
Fragments arranged by size:
867 663 495
PvuII CAG'CTG
Cuts at: 0 1624 2025
Size: 1624 401
SfcI C'TryA_G
Cuts at: 0 806 863 1176 1526 2025
Size: 806 57 313 350 499
Fragments arranged by size:
806 499 350 313 57
SmlI C'TyrA_G
Cuts at: 0 259 569 2025
Size: 259 310 1456
Fragments arranged by size:
1456 310 259
SspI AAT'ATT
Cuts at: 0 362 2025
Size: 362 1663
StuI AGG'CCT
Cuts at: 0 1174 2025
Size: 1174 851
StyI C'CwwG_G
Cuts at: 0 1028 2025
Size: 1028 997
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 2025
Size: 790 1235
TatI w'GTAC_w
Cuts at: 0 510 2025
Size: 510 1515
Tth111I GACn'n_nGTC
Cuts at: 0 829 2025
Size: 829 1196
XbaI T'CTAG_A
Cuts at: 0 903 2025
Size: 903 1122
XmnI GAAnn'nnTTC
Cuts at: 0 1811 2025
Size: 1811 214
Enzymes that do cut:
Acc65I AcuI AflII AhdI AloI ApaLI ApoI AseI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI
BsrFI BssSI BstAPI BstXI BstYI BtsI EaeI EarI
Eco57MI EcoRI FalI Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PacI
PpiI PshAI PsrI PstI PvuII SfcI SmlI SspI
StuI StyI TaqII TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BciVI BglI BmgBI BmtI BplI BsaAI
BsaBI BsaHI BseYI BsgI BsiEI BsiWI BsmBI BspMI
BsrBI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII
HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TstI XcmI XhoI XmaI ZraI