Restriction map for
pF1KB9495
(Linear) (Six-base) MAPSORT of: KIBB9495.seq Check: 8420 from: 1 to: 2508
KIBB9495.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 580 1361 2508
Size: 580 781 1147
Fragments arranged by size:
1147 781 580
AccI GT'mk_AC
Cuts at: 0 1579 2508
Size: 1579 929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 488 1324 1771 2348 2508
Size: 461 27 836 447 577 160
Fragments arranged by size:
836 577 461 447 160 27
AfeI AGC'GCT
Cuts at: 0 1434 2508
Size: 1434 1074
AflII C'TTAA_G
Cuts at: 0 1174 2508
Size: 1174 1334
AhdI GACnn_n'nnGTC
Cuts at: 0 461 602 2271 2508
Size: 461 141 1669 237
Fragments arranged by size:
1669 461 237 141
AleI CACnn'nnGTG
Cuts at: 0 2212 2508
Size: 2212 296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1312 1403 2508
Size: 1312 91 1105
Fragments arranged by size:
1312 1105 91
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 2508
Size: 289 32 2187
Fragments arranged by size:
2187 289 32
AlwNI CAG_nnn'CTG
Cuts at: 0 572 1327 1515 1779 2370 2508
Size: 572 755 188 264 591 138
Fragments arranged by size:
755 591 572 264 188 138
ApaI G_GGCC'C
Cuts at: 0 1913 2082 2508
Size: 1913 169 426
Fragments arranged by size:
1913 426 169
ApoI r'AATT_y
Cuts at: 0 1179 2508
Size: 1179 1329
AvaI C'yCGr_G
Cuts at: 0 53 79 131 1140 1680 1912 2508
Size: 53 26 52 1009 540 232 596
Fragments arranged by size:
1009 596 540 232 53 52 26
BamHI G'GATC_C
Cuts at: 0 508 2508
Size: 508 2000
BanI G'GyrC_C
Cuts at: 0 45 211 223 1296 2508
Size: 45 166 12 1073 1212
Fragments arranged by size:
1212 1073 166 45 12
BanII G_rGCy'C
Cuts at: 0 80 841 1140 1633 1913 2082 2508
Size: 80 761 299 493 280 169 426
Fragments arranged by size:
761 493 426 299 280 169 80
BbeI G_GCGC'C
Cuts at: 0 49 215 227 2508
Size: 49 166 12 2281
Fragments arranged by size:
2281 166 49 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 680 2508
Size: 680 1828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 2508
Size: 271 34 2203
Fragments arranged by size:
2203 271 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1205 1848 2508
Size: 1205 643 660
Fragments arranged by size:
1205 660 643
BglI GCCn_nnn'nGGC
Cuts at: 0 22 84 154 163 688 1322 1547 2158
Size: 22 62 70 9 525 634 225 611
Cuts at: 2158 2508
Size: 350
Fragments arranged by size:
634 611 525 350 225 70 62 22
9
BglII A'GATC_T
Cuts at: 0 633 2508
Size: 633 1875
BlpI GC'TnA_GC
Cuts at: 0 1436 2508
Size: 1436 1072
Bme1580I G_kGCm'C
Cuts at: 0 1301 1913 2082 2508
Size: 1301 612 169 426
Fragments arranged by size:
1301 612 426 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 803 1665 1688 2349 2508
Size: 277 526 862 23 661 159
Fragments arranged by size:
862 661 526 277 159 23
BmtI G_CTAG'C
Cuts at: 0 2165 2508
Size: 2165 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 193 316 472 1666 1728 1756 1801
Size: 55 138 123 156 1194 62 28 45
Cuts at: 1801 2508
Size: 707
Fragments arranged by size:
1194 707 156 138 123 62 55 45
28
Bpu10I CC'TnA_GC
Cuts at: 0 474 2006 2508
Size: 474 1532 502
Fragments arranged by size:
1532 502 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 1855 2437 2508
Size: 621 1234 582 71
Fragments arranged by size:
1234 621 582 71
BsaAI yAC'GTr
Cuts at: 0 2157 2508
Size: 2157 351
BsaBI GATnn'nnATC
Cuts at: 0 749 797 2508
Size: 749 48 1711
Fragments arranged by size:
1711 749 48
BsaHI Gr'CG_yC
Cuts at: 0 46 212 224 1550 1730 2508
Size: 46 166 12 1326 180 778
Fragments arranged by size:
1326 778 180 166 46 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 289 319 1113 1143 1884 1914
Size: 160 30 99 30 794 30 741 30
Cuts at: 1914 2508
Size: 594
Fragments arranged by size:
794 741 594 160 99 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 328 1556 1872 2088 2508
Size: 313 15 1228 316 216 420
Fragments arranged by size:
1228 420 316 313 216 15
BseYI C'CCAG_C
Cuts at: 0 256 1764 2508
Size: 256 1508 744
Fragments arranged by size:
1508 744 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 2508
Size: 853 1655
BsiEI CG_ry'CG
Cuts at: 0 17 2048 2091 2183 2508
Size: 17 2031 43 92 325
Fragments arranged by size:
2031 325 92 43 17
BsiHKAI G_wGCw'C
Cuts at: 0 449 2311 2508
Size: 449 1862 197
Fragments arranged by size:
1862 449 197
Bsp1286I G_dGCh'C
Cuts at: 0 80 449 841 1140 1301 1633 1913 2082
Size: 80 369 392 299 161 332 280 169
Cuts at: 2082 2311 2508
Size: 229 197
Fragments arranged by size:
392 369 332 299 280 229 197 169
161 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 580 1361 2508
Size: 188 392 781 1147
Fragments arranged by size:
1147 781 392 188
BsrBI CCG'CTC
Cuts at: 0 120 138 2508
Size: 120 18 2370
Fragments arranged by size:
2370 120 18
BsrDI GCAATG_nn'
Cuts at: 0 737 2508
Size: 737 1771
BsrFI r'CCGG_y
Cuts at: 0 48 2027 2508
Size: 48 1979 481
Fragments arranged by size:
1979 481 48
BsrGI T'GTAC_A
Cuts at: 0 1506 2508
Size: 1506 1002
BstBI TT'CG_AA
Cuts at: 0 1183 2508
Size: 1183 1325
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2214 2508
Size: 2214 294
BstYI r'GATC_y
Cuts at: 0 508 633 922 2462 2508
Size: 508 125 289 1540 46
Fragments arranged by size:
1540 508 289 125 46
Bsu36I CC'TnA_GG
Cuts at: 0 684 1320 1424 2071 2508
Size: 684 636 104 647 437
Fragments arranged by size:
684 647 636 437 104
BtgI C'CryG_G
Cuts at: 0 1592 1758 1937 1977 2210 2508
Size: 1592 166 179 40 233 298
Fragments arranged by size:
1592 298 233 179 166 40
BtsI GCAGTG_nn'
Cuts at: 0 1258 2508
Size: 1258 1250
DrdI GACnn_nn'nnGTC
Cuts at: 0 1416 2508
Size: 1416 1092
EaeI y'GGCC_r
Cuts at: 0 14 155 1364 1559 1974 1997 2045 2180
Size: 14 141 1209 195 415 23 48 135
Cuts at: 2180 2508
Size: 328
Fragments arranged by size:
1209 415 328 195 141 135 48 23
14
EagI C'GGCC_G
Cuts at: 0 14 2045 2180 2508
Size: 14 2031 135 328
Fragments arranged by size:
2031 328 135 14
EarI CTCTTCn'nnn_
Cuts at: 0 354 437 875 1592 1638 1891 2057 2508
Size: 354 83 438 717 46 253 166 451
Fragments arranged by size:
717 451 438 354 253 166 83 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 181 1389 2508
Size: 88 93 1208 1119
Fragments arranged by size:
1208 1119 93 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 193 316 461 472 488 1324 1666
Size: 55 138 123 145 11 16 836 342
Cuts at: 1666 1728 1756 1771 1801 2348 2508
Size: 62 28 15 30 547 160
Fragments arranged by size:
836 547 342 160 145 138 123 62
55 30 28 16 15 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 473 1306 1472 1663 2508
Size: 473 833 166 191 845
Fragments arranged by size:
845 833 473 191 166
EcoO109I rG'GnC_Cy
Cuts at: 0 1448 1477 2067 2078 2508
Size: 1448 29 590 11 430
Fragments arranged by size:
1448 590 430 29 11
EcoRI G'AATT_C
Cuts at: 0 1179 2508
Size: 1179 1329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1854 2508
Size: 1854 654
HaeII r_GCGC'y
Cuts at: 0 49 90 192 215 227 1328 1436 2508
Size: 49 41 102 23 12 1101 108 1072
Fragments arranged by size:
1101 1072 108 102 49 41 23 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 535 567 588 620 667 699
Size: 447 32 56 32 21 32 47 32
Cuts at: 699 760 792 1203 1235 2176 2208 2257 2289
Size: 61 32 411 32 941 32 49 32
Cuts at: 2289 2508
Size: 219
Fragments arranged by size:
941 447 411 219 61 56 49 47
32 32 32 32 32 32 32 32
21
KasI G'GCGC_C
Cuts at: 0 45 211 223 2508
Size: 45 166 12 2285
Fragments arranged by size:
2285 166 45 12
MfeI C'AATT_G
Cuts at: 0 340 2508
Size: 340 2168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1358 2508
Size: 629 729 1150
Fragments arranged by size:
1150 729 629
MscI TGG'CCA
Cuts at: 0 1976 1999 2508
Size: 1976 23 509
Fragments arranged by size:
1976 509 23
MslI CAynn'nnrTG
Cuts at: 0 905 2212 2508
Size: 905 1307 296
Fragments arranged by size:
1307 905 296
MspA1I CmG'CkG
Cuts at: 0 244 1760 1939 2508
Size: 244 1516 179 569
Fragments arranged by size:
1516 569 244 179
NaeI GCC'GGC
Cuts at: 0 50 2508
Size: 50 2458
NarI GG'CG_CC
Cuts at: 0 46 212 224 2508
Size: 46 166 12 2284
Fragments arranged by size:
2284 166 46 12
NgoMIV G'CCGG_C
Cuts at: 0 48 2508
Size: 48 2460
NheI G'CTAG_C
Cuts at: 0 2161 2508
Size: 2161 347
NspI r_CATG'y
Cuts at: 0 1390 2508
Size: 1390 1118
PflMI CCAn_nnn'nTGG
Cuts at: 0 1644 1779 2508
Size: 1644 135 729
Fragments arranged by size:
1644 729 135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 2182 2214 2278 2310 2508
Size: 289 32 1861 32 64 32 198
Fragments arranged by size:
1861 289 198 64 32 32 32
PspOMI G'GGCC_C
Cuts at: 0 1909 2078 2508
Size: 1909 169 430
Fragments arranged by size:
1909 430 169
PstI C_TGCA'G
Cuts at: 0 633 1547 2508
Size: 633 914 961
Fragments arranged by size:
961 914 633
SacII CC_GC'GG
Cuts at: 0 1761 1940 2508
Size: 1761 179 568
Fragments arranged by size:
1761 568 179
SapI GCTCTTCn'nnn_
Cuts at: 0 437 1638 2508
Size: 437 1201 870
Fragments arranged by size:
1201 870 437
SbfI CC_TGCA'GG
Cuts at: 0 1547 2508
Size: 1547 961
ScaI AGT'ACT
Cuts at: 0 2176 2508
Size: 2176 332
SexAI A'CCwGG_T
Cuts at: 0 1658 2508
Size: 1658 850
SfcI C'TryA_G
Cuts at: 0 629 1543 1580 2508
Size: 629 914 37 928
Fragments arranged by size:
928 914 629 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 154 2508
Size: 154 2354
SfoI GGC'GCC
Cuts at: 0 47 213 225 2508
Size: 47 166 12 2283
Fragments arranged by size:
2283 166 47 12
SmaI CCC'GGG
Cuts at: 0 133 1142 1682 2508
Size: 133 1009 540 826
Fragments arranged by size:
1009 826 540 133
SmlI C'TyrA_G
Cuts at: 0 600 1174 1870 2452 2508
Size: 600 574 696 582 56
Fragments arranged by size:
696 600 582 574 56
StuI AGG'CCT
Cuts at: 0 682 1605 2440 2508
Size: 682 923 835 68
Fragments arranged by size:
923 835 682 68
StyI C'CwwG_G
Cuts at: 0 587 1610 1886 2508
Size: 587 1023 276 622
Fragments arranged by size:
1023 622 587 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2465 2508
Size: 2465 43
TatI w'GTAC_w
Cuts at: 0 1506 2174 2508
Size: 1506 668 334
Fragments arranged by size:
1506 668 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1113 1145 1884 1916 2508
Size: 1113 32 739 32 592
Fragments arranged by size:
1113 739 592 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 974 1008 1250 2508
Size: 974 34 242 1258
Fragments arranged by size:
1258 974 242 34
XmaI C'CCGG_G
Cuts at: 0 131 1140 1680 2508
Size: 131 1009 540 828
Fragments arranged by size:
1009 828 540 131
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BglI BglII BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PflMI PpiI PspOMI PstI SacII SapI SbfI
ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BbvCI BclI BfrBI BmgBI BplI
BsaI BsaWI BsiWI BsmI BsmBI BspEI BspHI BssHII
BssSI BstAPI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EcoICRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MluI NcoI NdeI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI