Restriction map for
pF1KB9494
(Linear) (Six-base) MAPSORT of: KIBB9494.seq Check: 4354 from: 1 to: 2520
KIBB9494.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 646 824 2103 2520
Size: 646 178 1279 417
Fragments arranged by size:
1279 646 417 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 2520
Size: 1025 1495
AflIII A'CryG_T
Cuts at: 0 602 2520
Size: 602 1918
AleI CACnn'nnGTG
Cuts at: 0 217 2520
Size: 217 2303
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 2520
Size: 803 32 1685
Fragments arranged by size:
1685 803 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1452 2289 2328 2355 2445 2448 2520
Size: 1452 837 39 27 90 3 72
Fragments arranged by size:
1452 837 90 72 39 27 3
ApaI G_GGCC'C
Cuts at: 0 140 1512 2520
Size: 140 1372 1008
Fragments arranged by size:
1372 1008 140
ApaLI G'TGCA_C
Cuts at: 0 2389 2520
Size: 2389 131
ApoI r'AATT_y
Cuts at: 0 775 1993 2520
Size: 775 1218 527
Fragments arranged by size:
1218 775 527
AvaI C'yCGr_G
Cuts at: 0 75 1012 1082 2206 2520
Size: 75 937 70 1124 314
Fragments arranged by size:
1124 937 314 75 70
BanI G'GyrC_C
Cuts at: 0 164 193 1038 1108 2024 2139 2509 2520
Size: 164 29 845 70 916 115 370 11
Fragments arranged by size:
916 845 370 164 115 70 29 11
BanII G_rGCy'C
Cuts at: 0 140 635 829 1512 2520
Size: 140 495 194 683 1008
Fragments arranged by size:
1008 683 495 194 140
BbeI G_GCGC'C
Cuts at: 0 168 2513 2520
Size: 168 2345 7
Fragments arranged by size:
2345 168 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 2520
Size: 271 2249
BbvCI CC'TCA_GC
Cuts at: 0 1116 2520
Size: 1116 1404
BclI T'GATC_A
Cuts at: 0 1832 2520
Size: 1832 688
BglI GCCn_nnn'nGGC
Cuts at: 0 535 2044 2148 2520
Size: 535 1509 104 372
Fragments arranged by size:
1509 535 372 104
BlpI GC'TnA_GC
Cuts at: 0 1771 2434 2520
Size: 1771 663 86
Fragments arranged by size:
1771 663 86
Bme1580I G_kGCm'C
Cuts at: 0 140 198 1013 1043 1216 1512 2027 2144
Size: 140 58 815 30 173 296 515 117
Cuts at: 2144 2393 2520
Size: 249 127
Fragments arranged by size:
815 515 296 249 173 140 127 117
58 30
BmgBI CAC'GTC
Cuts at: 0 791 945 1332 2520
Size: 791 154 387 1188
Fragments arranged by size:
1188 791 387 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 341 419 1148 1465 2520
Size: 341 78 729 317 1055
Fragments arranged by size:
1055 729 341 317 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1875 2520
Size: 1875 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1474 1705 1996 2049 2311 2350 2520
Size: 604 870 231 291 53 262 39 170
Fragments arranged by size:
870 604 291 262 231 170 53 39
Bpu10I CC'TnA_GC
Cuts at: 0 1116 2520
Size: 1116 1404
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1765 2520
Size: 1765 755
BsaI GGTCTCn'nnnn_
Cuts at: 0 2412 2520
Size: 2412 108
BsaAI yAC'GTr
Cuts at: 0 1377 1530 2520
Size: 1377 153 990
Fragments arranged by size:
1377 990 153
BsaHI Gr'CG_yC
Cuts at: 0 165 1193 2510 2520
Size: 165 1028 1317 10
Fragments arranged by size:
1317 1028 165 10
BsaWI w'CCGG_w
Cuts at: 0 2087 2520
Size: 2087 433
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 1678 1708 2520
Size: 803 30 845 30 812
Fragments arranged by size:
845 812 803 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 1048 1072 1372 1851 2520
Size: 661 387 24 300 479 669
Fragments arranged by size:
669 661 479 387 300 24
BseYI C'CCAG_C
Cuts at: 0 641 976 1133 1344 2143 2505 2520
Size: 641 335 157 211 799 362 15
Fragments arranged by size:
799 641 362 335 211 157 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 916 1270 1387 1510 2329 2362 2520
Size: 336 580 354 117 123 819 33 158
Fragments arranged by size:
819 580 354 336 158 123 117 33
BsiEI CG_ry'CG
Cuts at: 0 368 908 1516 2431 2520
Size: 368 540 608 915 89
Fragments arranged by size:
915 608 540 368 89
BsiHKAI G_wGCw'C
Cuts at: 0 635 1571 1790 2393 2520
Size: 635 936 219 603 127
Fragments arranged by size:
936 635 603 219 127
Bsp1286I G_dGCh'C
Cuts at: 0 140 198 635 829 1013 1043 1216 1512
Size: 140 58 437 194 184 30 173 296
Cuts at: 1512 1571 1790 2027 2144 2393 2520
Size: 59 219 237 117 249 127
Fragments arranged by size:
437 296 249 237 219 194 184 173
140 127 117 59 58 30
BspHI T'CATG_A
Cuts at: 0 1835 2520
Size: 1835 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 646 824 1733 2103 2520
Size: 646 178 909 370 417
Fragments arranged by size:
909 646 417 370 178
BsrBI CCG'CTC
Cuts at: 0 1062 1984 2063 2520
Size: 1062 922 79 457
Fragments arranged by size:
1062 922 457 79
BsrFI r'CCGG_y
Cuts at: 0 342 2261 2520
Size: 342 1919 259
Fragments arranged by size:
1919 342 259
BsrGI T'GTAC_A
Cuts at: 0 545 1199 1247 1649 2520
Size: 545 654 48 402 871
Fragments arranged by size:
871 654 545 402 48
BssHII G'CGCG_C
Cuts at: 0 56 2520
Size: 56 2464
BssSI C'ACGA_G
Cuts at: 0 1288 2520
Size: 1288 1232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1734 2316 2376 2448 2520
Size: 1734 582 60 72 72
Fragments arranged by size:
1734 582 72 72 60
BstBI TT'CG_AA
Cuts at: 0 143 2520
Size: 143 2377
BstYI r'GATC_y
Cuts at: 0 563 668 2324 2520
Size: 563 105 1656 196
Fragments arranged by size:
1656 563 196 105
BtgI C'CryG_G
Cuts at: 0 531 539 695 863 1606 1718 2029 2520
Size: 531 8 156 168 743 112 311 491
Fragments arranged by size:
743 531 491 311 168 156 112 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 139 2520
Size: 139 2381
BtsI GCAGTG_nn'
Cuts at: 0 933 1788 2370 2500 2520
Size: 933 855 582 130 20
Fragments arranged by size:
933 855 582 130 20
DraIII CAC_nnn'GTG
Cuts at: 0 216 2520
Size: 216 2304
EaeI y'GGCC_r
Cuts at: 0 365 477 905 1513 1532 2032 2054 2520
Size: 365 112 428 608 19 500 22 466
Fragments arranged by size:
608 500 466 428 365 112 22 19
EagI C'GGCC_G
Cuts at: 0 365 905 1513 2520
Size: 365 540 608 1007
Fragments arranged by size:
1007 608 540 365
EarI CTCTTCn'nnn_
Cuts at: 0 769 1171 2520
Size: 769 402 1349
Fragments arranged by size:
1349 769 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1905 2520
Size: 1905 615
EcoICRI GAG'CTC
Cuts at: 0 633 2520
Size: 633 1887
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1025 1474 1705 1996 2049 2311 2350
Size: 604 421 449 231 291 53 262 39
Cuts at: 2350 2520
Size: 170
Fragments arranged by size:
604 449 421 291 262 231 170 53
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 315 2520
Size: 315 2205
EcoO109I rG'GnC_Cy
Cuts at: 0 137 320 335 1485 1508 2520
Size: 137 183 15 1150 23 1012
Fragments arranged by size:
1150 1012 183 137 23 15
EcoRI G'AATT_C
Cuts at: 0 775 1993 2520
Size: 775 1218 527
Fragments arranged by size:
1218 775 527
FspI TGC'GCA
Cuts at: 0 2499 2520
Size: 2499 21
HaeII r_GCGC'y
Cuts at: 0 168 852 1857 2513 2520
Size: 168 684 1005 656 7
Fragments arranged by size:
1005 684 656 168 7
HincII GTy'rAC
Cuts at: 0 2196 2520
Size: 2196 324
KasI G'GCGC_C
Cuts at: 0 164 2509 2520
Size: 164 2345 11
Fragments arranged by size:
2345 164 11
MfeI C'AATT_G
Cuts at: 0 611 2520
Size: 611 1909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 2520
Size: 1007 1513
MscI TGG'CCA
Cuts at: 0 479 1534 2520
Size: 479 1055 986
Fragments arranged by size:
1055 986 479
MslI CAynn'nnrTG
Cuts at: 0 217 887 938 1645 2386 2520
Size: 217 670 51 707 741 134
Fragments arranged by size:
741 707 670 217 134 51
MspA1I CmG'CkG
Cuts at: 0 846 1238 1403 1692 1770 2338 2445 2520
Size: 846 392 165 289 78 568 107 75
Fragments arranged by size:
846 568 392 289 165 107 78 75
NarI GG'CG_CC
Cuts at: 0 165 2510 2520
Size: 165 2345 10
Fragments arranged by size:
2345 165 10
NcoI C'CATG_G
Cuts at: 0 531 863 2520
Size: 531 332 1657
Fragments arranged by size:
1657 531 332
NspI r_CATG'y
Cuts at: 0 390 606 2202 2520
Size: 390 216 1596 318
Fragments arranged by size:
1596 390 318 216
PasI CC'CwG_GG
Cuts at: 0 1478 2520
Size: 1478 1042
PciI A'CATG_T
Cuts at: 0 602 2520
Size: 602 1918
PflMI CCAn_nnn'nTGG
Cuts at: 0 1452 1932 2520
Size: 1452 480 588
Fragments arranged by size:
1452 588 480
PfoI T'CCnGG_A
Cuts at: 0 620 1538 1955 2327 2520
Size: 620 918 417 372 193
Fragments arranged by size:
918 620 417 372 193
PmlI CAC'GTG
Cuts at: 0 1530 2520
Size: 1530 990
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 803 835 2520
Size: 803 32 1685
Fragments arranged by size:
1685 803 32
PpuMI rG'GwC_Cy
Cuts at: 0 335 2520
Size: 335 2185
PspOMI G'GGCC_C
Cuts at: 0 136 1508 2520
Size: 136 1372 1012
Fragments arranged by size:
1372 1012 136
PstI C_TGCA'G
Cuts at: 0 1730 1769 2270 2324 2520
Size: 1730 39 501 54 196
Fragments arranged by size:
1730 501 196 54 39
PvuII CAG'CTG
Cuts at: 0 1692 1770 2338 2445 2520
Size: 1692 78 568 107 75
Fragments arranged by size:
1692 568 107 78 75
SacI G_AGCT'C
Cuts at: 0 635 2520
Size: 635 1885
SanDI GG'GwC_CC
Cuts at: 0 335 2520
Size: 335 2185
SapI GCTCTTCn'nnn_
Cuts at: 0 1171 2520
Size: 1171 1349
SexAI A'CCwGG_T
Cuts at: 0 1826 2520
Size: 1826 694
SfcI C'TryA_G
Cuts at: 0 1726 1765 2266 2320 2520
Size: 1726 39 501 54 200
Fragments arranged by size:
1726 501 200 54 39
SfoI GGC'GCC
Cuts at: 0 166 2511 2520
Size: 166 2345 9
Fragments arranged by size:
2345 166 9
SmlI C'TyrA_G
Cuts at: 0 1780 2520
Size: 1780 740
StuI AGG'CCT
Cuts at: 0 1714 2155 2520
Size: 1714 441 365
Fragments arranged by size:
1714 441 365
StyI C'CwwG_G
Cuts at: 0 65 531 863 1919 2402 2520
Size: 65 466 332 1056 483 118
Fragments arranged by size:
1056 483 466 332 118 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 641 1147 1336 2042 2520
Size: 181 460 506 189 706 478
Fragments arranged by size:
706 506 478 460 189 181
TatI w'GTAC_w
Cuts at: 0 545 1199 1247 1649 1703 2520
Size: 545 654 48 402 54 817
Fragments arranged by size:
817 654 545 402 54 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 1652 1678 1684 1710 2520
Size: 775 32 845 26 6 26 810
Fragments arranged by size:
845 810 775 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 0 109 2520
Size: 109 2411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2133 2520
Size: 2133 387
XmnI GAAnn'nnTTC
Cuts at: 0 1846 2520
Size: 1846 674
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII HincII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SanDI SapI SexAI SfcI SfoI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BfrBI BglII BmtI BsaBI BsiWI BsmI BsmBI
BspEI BsrDI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DrdI EcoRV FalI FseI FspAI Hin4I HindIII
HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI ZraI